The Role of Atrial Natriuretic Peptide in the Lipolytic Process: Effect of Obesity and Exercise

NCT02418728 | Completed

• 1 other identifier: CME2014 /450
• Study Type: observational
• Target: 30
• Locations: 1 country
• Sites: 1

Brief Summary

In the developing countries, obesity prevalence is on a dramatic rise. Obesity is related to co-morbidities and as a result, obesity significantly shortens life expectancy and lowers quality of life. To prevent this, participation in exercise or training programs is absolutely necessary, in order to generate adipose tissue mass loss. The amount of adipose tissue mass loss is, amongst others, dependent on lipolysis which is under endocrine regulation by, mainly, catecholamines, insulin and atrial natriuretic peptide. However, large variations in adipose tissue mass loss and gain are likely in obese subjects, possibly due to a decreased lipolytic effect of these hormones (as was shown for catecholamines in the subcutaneous adipose tissue of obese subjects). However, the relative contribution of atrial natriuretic peptide in the lipolytic process remains elusive, particularly in subjects with obesity, which show an increased plasma expression of atrial natriuretic peptide. The aim of the present study is to observe the contribution of atrial natriuretic peptide in the subcutaneous adipose tissue of obese subjects. This will be tested by measurements of extracellular glycerol levels (by microdialysis) in the subcutaneous adipose tissue in situ at rest and during endurance exercise under local beta- and alpha-blockade. Eventually, the knowledge gained from this research will contribute to the optimization of exercise programs for people with obesity.

Conditions

Obesity

Keywords

obesity; adipose tissue lipolysis; lipolysis; microdialysis; atrial natriuretic peptide

Outcome Measures

Primary Outcomes (2)

• Subcutaneous adipose tissue microdialysis

  abdominal subcutaneous adipose tissue microdialysis during rest and exercise; basal + under local alpha- and beta-blockade.

  week 1

• Atrial natriuretic peptide (ANP) Response

  measurement systemic ANP response (venous blood sampling)

  week 1

Secondary Outcomes (5)

• Central insulin sensitivity

  screening

• Echocardiography

  Day 1

• Maximal oxygen uptake (ml/O2/kg/min)

  Day 1

• Anthropometry

  screening

• Abdominal subcutaneous adipose tissue biopsy

  week 1

Study Arms (2)

persons with obesity

Other: subcutaneous adipose tissue microdialysis under local beta/alpha blockade

lean persons

Other: subcutaneous adipose tissue microdialysis under local beta/alpha blockade

Interventions

subcutaneous adipose tissue microdialysis under local beta/alpha blockade OTHER

adipose tissue lipolysis under local beta/alpha blockade in abdominal subcutaneous adipose tissue, using microdialysis

lean persons; persons with obesity

Eligibility Criteria

• Age: 35 Years - 60 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

subjects with obesity

You may qualify if:

• Obese group: BMI \> 30 kg/m², sedentary (no regular physical activity last 6 months), insulin sensitive or insulin resistant
• Lean controls: BMI \> 18.5 kg/m² and \< 25 kg/m², regular physical activity, insulin sensitive

You may not qualify if:

• Regular glucose lowering medication or beta blockade medication
• Presence of chronical diseases
• Orthopedic or neurological problems

Sponsors & Collaborators

• Hasselt University: lead
• Jessa Hospital: collaborator
• Maastricht University: collaborator

Study Sites (1)

Hasselt University

Diepenbeek, 3590, Belgium

Location

MeSH Terms

Conditions

Obesity

Condition Hierarchy (Ancestors)

Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Study Officials

• Ellen Blaak, prof. dr.

  Maastricht University

  PRINCIPAL INVESTIGATOR

• Kenneth Verboven, drs

  Hasselt University

  STUDY CHAIR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: RETROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: prof. dr.

Study Record Dates

• First Submitted: March 17, 2015
• First Posted: April 16, 2015
• Study Start: August 1, 2014
• Primary Completion: August 1, 2016
• Study Completion: November 1, 2016
• Last Updated: November 18, 2016

Record last verified: 2016-11

Locations

BE (1)