NCT02384148

Brief Summary

The investigators aimed to characterize human circadian clockwork in healthy individuals in comparison to obese and type 2 diabetic subjects, employing the noninvasive experimental approach allowing the study of circadian profile in human individuals.

Trial Health

55
Monitor

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
300

participants targeted

Target at P75+ for all trials

Geographic Reach
1 country

1 active site

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

June 1, 2011

Completed
3.7 years until next milestone

First Submitted

Initial submission to the registry

February 27, 2015

Completed
11 days until next milestone

First Posted

Study publicly available on registry

March 10, 2015

Completed
2.7 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 1, 2017

Completed
Last Updated

December 2, 2015

Status Verified

December 1, 2015

Enrollment Period

6.5 years

First QC Date

February 27, 2015

Last Update Submit

December 1, 2015

Conditions

Outcome Measures

Primary Outcomes (1)

  • Difference of period length

    Period length will be calculated from oscillation profiles of Bmal1-luciferase or Per2-luc luciferase.

    5 days

Secondary Outcomes (1)

  • Difference of amplitude

    5 days

Study Arms (4)

Healthy

Healthy volunteers with no inflammation diseases, no neoplasia, no allergy to lidocaine.

Other: Circadian rhythm measurementProcedure: Skin biopsy

Type 2 diabetic non obese

Type 2 diabetic patients with HbA1c\>6.5%, body mass index 19-30 kg/m2, no inflammation diseases, no neoplasia, no allergy to lidocaine.

Other: Circadian rhythm measurementProcedure: Skin biopsy

Type 2 diabetic obese

Type 2 diabetic patients with HbA1c\>6.5%, body mass index \>30 kg/m2, no inflammation diseases, no neoplasia, no allergy to lidocaine.

Other: Circadian rhythm measurementProcedure: Skin biopsy

obese

Obese non diabetic patients with HbA1c\<6.5%, body mass index \>30 kg/m2, no inflammation diseases, no neoplasia, no allergy to lidocaine.

Other: Circadian rhythm measurementProcedure: Skin biopsy

Interventions

HealthyType 2 diabetic non obeseType 2 diabetic obeseobese
Skin biopsyPROCEDURE

A 2mm diameter skin biopsy is performed by a physician using standard procedure.

HealthyType 2 diabetic non obeseType 2 diabetic obeseobese

Eligibility Criteria

Age20 Years - 80 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

One hundred normolipidemic healthy individuals, one hundred T2D subjects divided into two sub-groups (T2D non-obese and T2D obese) and one hundred obese non diabetic patients will be included.

You may qualify if:

  • age 20-80
  • consent obtained

You may not qualify if:

  • neoplasia
  • inflammatory disease
  • allergy to lidocaine

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Geneva University Hospital

Geneva, 1205, Switzerland

Location

Related Publications (2)

  • Pagani L, Schmitt K, Meier F, Izakovic J, Roemer K, Viola A, Cajochen C, Wirz-Justice A, Brown SA, Eckert A. Serum factors in older individuals change cellular clock properties. Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):7218-23. doi: 10.1073/pnas.1008882108. Epub 2011 Apr 11.

    PMID: 21482780BACKGROUND
  • Brown SA, Fleury-Olela F, Nagoshi E, Hauser C, Juge C, Meier CA, Chicheportiche R, Dayer JM, Albrecht U, Schibler U. The period length of fibroblast circadian gene expression varies widely among human individuals. PLoS Biol. 2005 Oct;3(10):e338. doi: 10.1371/journal.pbio.0030338. Epub 2005 Sep 27.

    PMID: 16167846BACKGROUND

MeSH Terms

Conditions

Diabetes MellitusObesity

Condition Hierarchy (Ancestors)

Glucose Metabolism DisordersMetabolic DiseasesNutritional and Metabolic DiseasesEndocrine System DiseasesOverweightOvernutritionNutrition DisordersBody WeightSigns and SymptomsPathological Conditions, Signs and Symptoms

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
CROSS SECTIONAL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
phD

Study Record Dates

First Submitted

February 27, 2015

First Posted

March 10, 2015

Study Start

June 1, 2011

Primary Completion

December 1, 2017

Last Updated

December 2, 2015

Record last verified: 2015-12

Locations