Analysis of CA-MRSA Transmission: An ED Population Sampling Strategy

NCT02363166 | Completed

• 1 other identifier: IRB201400426
• Study Type: observational
• Target: 575
• Locations: 1 country
• Sites: 1

Brief Summary

Given that the Emergency Department (ED) has become the entry way for large populations of patients into the health care system, a strategy of sampling MRSA isolates in ED populations and merging this information with patient-level data may present a window to hypothesize and investigate CA-MRSA transmission within the community and its impact on hospital-acquired infections.

Conditions

Methicillin-Resistant Staphylococcus Aureus

Outcome Measures

Primary Outcomes (1)

• Strain Relatedness Identification Through Phylogenetic Analysis

  Molecular (spa-typing) and genomic (WGS) relatedness of MRSA strains in isolates in ED populations presenting with SSTIs.

  1 Hour

Secondary Outcomes (3)

• Strain Transmission Through Analysis of Genetic Clustering

  1 year

• Patient Level Characteristics of Skin and Soft Tissue Infection (SSTI) Presentation

  6 Months

• Pediatric and Adult Population Presentation Characteristics in Skin and Soft Tissue Infection (SSTI)

  6 Months

Study Arms (1)

Acute Abscess Group

Adults and pediatric patients presenting to the UFHealth Shands Emergency Department with evidence of an acute abscess, or skin/soft tissue infection, which can be sampled for culture and sensitivity testing will be recruited.

Other: Acute Abscess Group

Interventions

Acute Abscess Group OTHER

Samples collected for culture and sensitivity testing depending on acute abscess, or skin/soft tissue infection.

Acute Abscess Group

Eligibility Criteria

• Age: 1 Month+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Adult and pediatric patients presenting to the UFHealth Shands Emergency Department with evidence of an acute abscess, or skin/soft tissue infection (SSTI), which can be sampled for culture and sensitivity testing

You may qualify if:

• Patient or Legally Authorized Representative (LAR) must have voluntarily signed an Institutional Review Board-approved informed consent form before initiation of any study procedures
• Patient presents with an acute abscess or a non-post-operative skin/soft tissue infection
• Patient presents through the UFHealth Shands Emergency Department

You may not qualify if:

• Patients who are employed by UFHealth and provide direct patient care
• Patients who have previously been enrolled in the study
• Patients who are not suitable for the study in the opinion of the investigator

Sponsors & Collaborators

• University of Florida: lead

Study Sites (1)

UF Health

Gainesville, Florida, 32608, United States

Location

Related Publications (5)

• Burton DC, Edwards JR, Horan TC, Jernigan JA, Fridkin SK. Methicillin-resistant Staphylococcus aureus central line-associated bloodstream infections in US intensive care units, 1997-2007. JAMA. 2009 Feb 18;301(7):727-36. doi: 10.1001/jama.2009.153.

  PMID: 19224749 BACKGROUND

• Climo MW. Decreasing MRSA infections: an end met by unclear means. JAMA. 2009 Feb 18;301(7):772-3. doi: 10.1001/jama.2009.149. No abstract available.

  PMID: 19224756 BACKGROUND

• Prosperi M, Veras N, Azarian T, Rathore M, Nolan D, Rand K, Cook RL, Johnson J, Morris JG Jr, Salemi M. Molecular epidemiology of community-associated methicillin-resistant Staphylococcus aureus in the genomic era: a cross-sectional study. Sci Rep. 2013;3:1902. doi: 10.1038/srep01902.

  PMID: 23712667 BACKGROUND

• Park SH, Park C, Yoo JH, Choi SM, Choi JH, Shin HH, Lee DG, Lee S, Kim J, Choi SE, Kwon YM, Shin WS. Emergence of community-associated methicillin-resistant Staphylococcus aureus strains as a cause of healthcare-associated bloodstream infections in Korea. Infect Control Hosp Epidemiol. 2009 Feb;30(2):146-55. doi: 10.1086/593953.

  PMID: 19128184 BACKGROUND

• Wu D, Wang Q, Yang Y, Geng W, Wang Q, Yu S, Yao K, Yuan L, Shen X. Epidemiology and molecular characteristics of community-associated methicillin-resistant and methicillin-susceptible Staphylococcus aureus from skin/soft tissue infections in a children's hospital in Beijing, China. Diagn Microbiol Infect Dis. 2010 May;67(1):1-8. doi: 10.1016/j.diagmicrobio.2009.12.006. Epub 2010 Mar 12.

  PMID: 20227225 BACKGROUND

Biospecimen

Retention: SAMPLES WITH DNA

Genomic DNA (gDNA) will be isolated from pelleted bacteria using the Roche High Pure PCR kit following the standard protocol for isolation of nucleic acids from bacteria. The quality of the extracted gDNA will be determined through gel electrophoresis and the quantity through Nanodrop2000.

Study Officials

• Joseph A Tyndall, MD

  University of Florida

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE ONLY
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: February 9, 2015
• First Posted: February 13, 2015
• Study Start: August 1, 2015
• Primary Completion: April 4, 2018
• Study Completion: April 4, 2018
• Last Updated: August 21, 2024

Record last verified: 2024-08

Locations

US (1)