Recolonisation of Bacteria in Hands and Possible Bacterial Leakage From Glove Cuff in Cardiac Surgery

NCT02359708 | Completed

• 1 other identifier: glovecuff2014
• Study Type: interventional
• Target: 28
• Locations: 1 country
• Sites: 1

Brief Summary

This study aims to investigate if recolonisation of the hand flora occur after surgical hand washing and is there a bacterial leakage at the glove cuff site in cardiac surgery? Also investigate if thera are differences in microbial flora and recolonisation between hospital and non-hospital persons.

Conditions

Bacterial Growth

Outcome Measures

Primary Outcomes (1)

• Leakage from gloves at the glove cuff site as bacterial growth cfu/mL

  Cultures taken after hand wash and after wearing surgical gloves for about 3 hours

  3 to 4 hours

Secondary Outcomes (1)

• Differences between hand flora and its recolonisation, between hospital staff and non-hospital persons showed as bacterial growth, cfu/mL.

  2-3 hours

Study Arms (2)

OR nurse group

EXPERIMENTAL

OR nurses (14) who agreed to participate were asked to perform a surgical hand disinfection accordingly to clinic routine. When hands were dry cultures were first obtained at 3 sites, using a moist with saline nylon flocked swab (Copan ESwab, Italia SpA). After hand disinfection; 1) in right hand palm, 2) between index finger and middle finger, 3) nail/cuticle of index finger. When surgery were completed and before disposal of gloves and gown the fourth culture were taken where the glove cuff meets the gown sleeve, under and above, the inner glove of all OR nurses.When gloves were removed cultures were taken again at three sites on the hand, approximately 2-3 hours.

Procedure: Surgical hand disinfection.

Non-hospital group

EXPERIMENTAL

Non-hospital volunteers who agreed to participate were asked to perform a surgical hand disinfection accordingly to clinic routine. When hands were dry cultures were first obtained at 3 sites, using a moist with saline nylon flocked swab (Copan ESwab, Italia SpA). After hand disinfection; 1) in right hand palm, 2) between index finger and middle finger, 3) nail/cuticle of index finger. This group whore gowns and gloves for approximately 2-3 hours but not kept sterile. The Culture at the glove cuff were left out. When gloves were removed cultures were taken again at three sites on the hand.

Procedure: Surgical hand disinfection.

Interventions

Surgical hand disinfection. PROCEDURE

Bacterial recolonization

Non-hospital group; OR nurse group

Eligibility Criteria

• Age: 18 Years - 65 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Operating nurse at the department and non-hospital persons

You may not qualify if:

• Participated previously in present study
• Use of gloves with antimicrobial effect
• Patient with a known infection

Sponsors & Collaborators

• Örebro University, Sweden: lead

Study Sites (1)

Örbro university hospital

Örebro, 701 85, Sweden

Location

Related Publications (1)

• Wistrand C, Soderquist B, Falk-Brynhildsen K, Nilsson U. Exploring bacterial growth and recolonization after preoperative hand disinfection and surgery between operating room nurses and non-health care workers: a pilot study. BMC Infect Dis. 2018 Sep 17;18(1):466. doi: 10.1186/s12879-018-3375-3.

  PMID: 30223772 DERIVED

MeSH Terms

Interventions

Hand Disinfection

Intervention Hierarchy (Ancestors)

Hand Hygiene; Hygiene; Public Health; Environment and Public Health

Study Officials

• Camilla Wistrand, phd student

  Örebro University, Sweden

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: NONE
• Purpose: SCREENING
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Operating room nurse

Study Record Dates

• First Submitted: January 21, 2015
• First Posted: February 10, 2015
• Study Start: February 1, 2014
• Primary Completion: May 1, 2015
• Study Completion: May 1, 2015
• Last Updated: April 4, 2017

Record last verified: 2017-04

Locations

SE (1)