SEGATROM: Sensory and Gastrointestinal Impact of Taste Receptor Variants on Human Metabolism and Nutrition

NCT02219295 | Completed

• 1 other identifier: BMBF 11005D
• Study Type: interventional
• Target: 97
• Locations: 1 country
• Sites: 1

Brief Summary

We investigate the impact of taste receptors (sweet, bitter and umami) on human metabolism. There ist growing evidence, that components of the taste signalling system, including the taste receptor proteins , have important functions in the regulation of metabolic parameters in non-gustatory tissues.

Conditions

Disorder of Glucose Regulation

Keywords

SGLT1 (sodium dependent glucose transporter); Glut-transporters; taste receptors genes; sweet taste receptor T1R2T1R3; bitter taste receptors of the hTAS2gene family

Outcome Measures

Primary Outcomes (1)

• Area under the curve (AUC): changes of expression levels of the sweet , bitter und umami taste receptors in humans in the upper bowel and responses of incretins to receptor agonists and antagonists

  measurement of incretins (GIP, GLP-1) and peptide hormones in human plasma after consumption of different sugars , sweeteners , bitter tasting ingredients of vegetables und umami. Measurement of expression levels in human intestinal mucosa before and after receptor stimulation

  three years

Secondary Outcomes (1)

• changes in the signalling cascade of the sweet receptor SGLT1 (sodium tranporter 1), changes of receptor expression

  3 years

Other Outcomes (1)

• Changes in the Area under the Curve (AUC) for PYY in different bitter tasting substances

  3 years

Study Arms (4)

sweet -block

EXPERIMENTAL

application of different sugars, artificial sweeteners and sweet-taste antagonists in a single dose in 300 ml tap water every week up to 7 times and collection of blood samples over 3 hours

Dietary Supplement: sweet block; Dietary Supplement: bitter block; Dietary Supplement: umami-block

bitter block

EXPERIMENTAL

application of purified bitter tasting ingredients of vegetables and hop in 300 ml tap water , collection of blood samples for 3h (-15,0,15,30,60,120,180 min)

Dietary Supplement: sweet block; Dietary Supplement: bitter block; Dietary Supplement: umami-block

umami-block

EXPERIMENTAL

application of glutamate and glutamate +IMP same procedure as above

Dietary Supplement: sweet block; Dietary Supplement: bitter block; Dietary Supplement: umami-block

gastroscopy

OTHER

gastroscopy with and without oral intervention with artificial sweetener saccharin to take tissue samples from the upper bowel for the investigation of taste receptor cells in the upper bowel in human beings

Other: gastroscopy

Interventions

sweet block DIETARY_SUPPLEMENT

oral application of the above mentioned substances in 300 ml tap water in 5 min. , collection of blood at 0,15,30, 60 ,120,180 minutes for measurement of incretins GIP(gastrointestinal polypeptide) and GLP-1 (glucagon like peptide 1), Protein hormones PYY(Peptide YY),CCK (cholecystokinin), Leptin and Insulin

Also known as: Sugars as sucrose, glucose, palatinose, galactose, artificial sweeteners as saccharin, sweet taste antagonist as lactisol

bitter block; sweet -block; umami-block

bitter block DIETARY_SUPPLEMENT

oral application of the above mentioned substances in 300 ml tap water, collection of blood samples at -15,0,15,30,60,120,180 min for Insulin, GIP,GLP-1, PYY and glucose

Also known as: application of, sinigrin, lactucopicrin, iso-alpha-acid

bitter block; sweet -block; umami-block

umami-block DIETARY_SUPPLEMENT

oral application of umami substances as glutamate with and without IMP, collection of blood samples as above

Also known as: application of glutamate and glutamate+IMP,

bitter block; sweet -block; umami-block

gastroscopy OTHER

gastroscopy with and without oral application of saccharin, tissue samples from th upper duodenum by endoscopy for the detection of taste receptor cells in the upper bowel

Also known as: tissue samples, sweet taste receptor stimualtion, artificial sweetener saccharin, SGLT-1

gastroscopy

Eligibility Criteria

• Age: 18 Years - 75 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Healthy persons

You may not qualify if:

• Tumor in history
• Pregnancy
• Severe illness in history
• Bodymass index below 18

Sponsors & Collaborators

• German Institute of Human Nutrition: lead
• Technical University of Munich: collaborator

Study Sites (1)

Department of Clinical Nutrition

Bergholz-Rehbrücke, Brandenburg, D-14558, Germany

Location

Related Publications (2)

• Zhang J, Schafer SM, Kabisch S, Csanalosi M, Schuppelius B, Kemper M, Markova M, Meyer NMT, Pivovarova-Ramich O, Keyhani-Nejad F, Rohn S, Pfeiffer AFH. Implication of sugar, protein and incretins in excessive glucagon secretion in type 2 diabetes after mixed meals. Clin Nutr. 2023 Apr;42(4):467-476. doi: 10.1016/j.clnu.2023.02.011. Epub 2023 Feb 21.

  PMID: 36857956 DERIVED

• Keyhani-Nejad F, Kemper M, Schueler R, Pivovarova O, Rudovich N, Pfeiffer AF. Effects of Palatinose and Sucrose Intake on Glucose Metabolism and Incretin Secretion in Subjects With Type 2 Diabetes. Diabetes Care. 2016 Mar;39(3):e38-9. doi: 10.2337/dc15-1891. Epub 2015 Dec 30. No abstract available.

  PMID: 26721819 DERIVED

MeSH Terms

Interventions

Sugars; Sucrose; Glucose; isomaltulose; Galactose; Sweetening Agents; Saccharin; sinigrin; intybin; oxytocin, 1-alpha-mercaptoacetic acid-iso-Asn(5)-; Gastroscopy; Sodium-Glucose Transporter 1

Intervention Hierarchy (Ancestors)

Carbohydrates; Disaccharides; Oligosaccharides; Polysaccharides; Hexoses; Monosaccharides; Flavoring Agents; Food Additives; Food Ingredients; Specialty Uses of Chemicals; Chemical Actions and Uses; Food; Diet, Food, and Nutrition; Physiological Phenomena; Food and Beverages; Thiazoles; Sulfur Compounds; Organic Chemicals; Benzothiazoles; Azoles; Heterocyclic Compounds, 1-Ring; Heterocyclic Compounds; Heterocyclic Compounds, 2-Ring; Heterocyclic Compounds, Fused-Ring; Endoscopy, Gastrointestinal; Endoscopy, Digestive System; Diagnostic Techniques, Digestive System; Diagnostic Techniques and Procedures; Diagnosis; Endoscopy; Diagnostic Techniques, Surgical; Digestive System Surgical Procedures; Surgical Procedures, Operative; Minimally Invasive Surgical Procedures; Sodium-Glucose Transport Proteins; Symporters; Ion Pumps; Membrane Transport Proteins; Carrier Proteins; Proteins; Amino Acids, Peptides, and Proteins; Monosaccharide Transport Proteins; Solute Carrier Proteins; Membrane Proteins

Study Officials

• Andreas FH Peiffer, Prof

  German Institute of Human Nutrition

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Purpose: BASIC SCIENCE
• Intervention Model: FACTORIAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Prof. Dr. med.

Study Record Dates

• First Submitted: August 1, 2014
• First Posted: August 18, 2014
• Study Start: December 1, 2011
• Primary Completion: September 1, 2014
• Study Completion: September 1, 2014
• Last Updated: June 24, 2020

Record last verified: 2020-06

Locations

DE (1)