NCT02028676

Brief Summary

The two original objectives were to determine in HIV-infected children initiating antiretroviral therapy (ART):

  1. 1.Whether clinically driven monitoring (CDM) will have a similar outcome in terms of disease progression or death as routine laboratory and clinical monitoring (LCM) for toxicity (haematology/biochemistry) and efficacy (CD4)?
  2. 2.Whether induction with four drugs from two ART classes followed by maintenance with three drugs after 36 weeks be more effective than a continuous non-nucleoside reverse transcriptase inhibitors (NNRTI)-based triple drug regimen in terms of CD4 and clinical outcome?
  3. 3.Whether changing from twice daily lamivudine+abacavir to once daily lamivudine+abacavir after 48 weeks on ART will have a similar outcome in terms of virological suppression and will result in improvements in adherence to ART?
  4. 4.Whether stopping daily cotrimoxazole prophylaxis in children over 3 years of age who have been on ART for at least 96 weeks has a similar outcome in terms of hospitalisation or death as continuing daily cotrimoxazole?

Trial Health

90
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
1,206

participants targeted

Target at P75+ for phase_4

Timeline
Completed

Started Mar 2007

Longer than P75 for phase_4

Geographic Reach
2 countries

4 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

March 1, 2007

Completed
5 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

March 1, 2012

Completed
3 months until next milestone

Study Completion

Last participant's last visit for all outcomes

June 1, 2012

Completed
1.6 years until next milestone

First Submitted

Initial submission to the registry

December 31, 2013

Completed
7 days until next milestone

First Posted

Study publicly available on registry

January 7, 2014

Completed
5 months until next milestone

Results Posted

Study results publicly available

June 6, 2014

Completed
Last Updated

June 6, 2014

Status Verified

June 1, 2014

Enrollment Period

5 years

First QC Date

December 31, 2013

Results QC Date

January 15, 2014

Last Update Submit

June 4, 2014

Conditions

Keywords

HIVAfricachildrenantiretroviral therapylaboratory monitoringtoxicityCD4induction maintenancecotrimoxazoleprophylaxisabacavirlamivudineonce daily

Outcome Measures

Primary Outcomes (9)

  • LCM vs CDM: Disease Progression to a New WHO Stage 4 Event or Death

    Number of participants with disease progression to a new WHO stage 4 event or death, to be analysed using time-to-event methods

    Median 4 years (from randomization to 16 March 2012; maximum 5 years)

  • LCM vs CDM: New Grade 3 or 4 Adverse Event (AE), Not Solely Related to HIV

    Number of participants with a new Grade 3 or 4 adverse event (AE), not solely related to HIV, to be analysed using time-to-event methods

    Median 4 years (from randomization to 16 March 2012; maximum 5 years)

  • Induction ART: Change From Baseline in CD4% 72 Weeks After ART Initiation

    Baseline, 72 weeks

  • Induction ART: Change From Baseline in CD4% to 144 Weeks From ART Initiation

    Baseline, 144 weeks

  • Induction ART: New Grade 3 or 4 Adverse Event (AE), Not Solely Related to HIV

    Number of participants with a new grade 3 or 4 adverse event (AE), not solely related to HIV, to be analysed using time-to-event methods

    Median 4 years (from randomization to 16 March 2012; maximum 5 years)

  • Once Versus Twice Daily Abacavir+Lamivudine: Suppressed HIV RNA Viral Load 48 Weeks After Randomisation

    Number of participants with HIV RNA viral load \<80 copies/ml at 48 weeks. Measured retrospectively on stored plasma specimens: due to low stored volumes from some children, samples had to be diluted and therefore a threshold of \<80 copies/ml was used to indicate suppression.

    48 weeks

  • Once Versus Twice Daily Abacavir+Lamivudine: New Grade 3 or 4 Adverse Event (AE), Not Solely Related to HIV, Judged Definitely/Probably or Uncertain Whether Related to Lamivudine or Abacavir

    Number of participants with a new grade 3 or 4 adverse event (AE), not solely related to HIV, judged definitely/probably or uncertain whether related to lamivudine or abacavir, to be analysed using time-to-event methods

    Median 2 years (from once vs twice daily randomization to 16 March 2012; maximum 2.6 years)

  • Cotrimoxazole: New Hospitalisation or Death

    Number of participants with a new hospitalisation or death, to be analysed using time-to-event methods

    Median 2 years (from cotrimoxazole randomization to 16 March 2012; maximum 2.5 years)

  • Cotrimoxazole: New Grade 3 or 4 Adverse Event (AE), Not Solely Related to HIV

    Number of participants with a new grade 3 or 4 adverse event (AE), not solely related to HIV, to be analysed using time-to-event methods

    Median 2 years (from cotrimoxazole randomization to 16 March 2012; maximum 2.5 years)

Secondary Outcomes (49)

  • LCM vs CDM, Induction ART: All-cause Mortality

    Median 4 years (from randomization to 16 March 2012; maximum 5 years)

  • Induction ART: New WHO Stage 4 Event or Death

    Median 4 years (from randomization to 16 March 2012; maximum 5 years)

  • LCM vs CDM, Induction ART: New WHO Stage 3 or 4 Event or Death

    Median 4 years (from randomization to 16 March 2012; maximum 5 years)

  • LCM vs CDM, Induction ART: New or Recurrent WHO Stage 3 or 4 Event or Death

    Median 4 years (from randomization to 16 March 2012; maximum 5 years)

  • LCM vs CDM, Induction ART: Weight-for-age Z-score

    Baseline and a median of 4 years (maximum 5 years)

  • +44 more secondary outcomes

Study Arms (9)

Clinically Driven Monitoring (CDM)

EXPERIMENTAL
Other: Clinically Driven Monitoring (CDM)

Laboratory plus Clinical Monitoring (LCM)

ACTIVE COMPARATOR
Other: Laboratory plus Clinical Monitoring (LCM)

Arm A: abacavir (ABC)+lamivudine (3TC)+NNRTI

ACTIVE COMPARATOR

ABC \[abacavir\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO 3TC \[lamivudine\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO non-nucleoside reverse transcriptase inhibitor (NNRTI): either nevirapine or efavirenz based on availability (provided by national ART programmes in Uganda/Zimbabwe). Nevirapine syrup or tablet dosed twice-daily according to weight-bands following WHO. Efavirenz tablets dosed once-daily according to weight-bands following WHO.

Drug: Arm A: ABC+3TC+NNRTI

Arm B: ZDV+ABC+3TC+NNRTI->ABC+3TC+NNRTI maintenance

EXPERIMENTAL

ZDV \[abacavir\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO ABC \[abacavir\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO 3TC \[lamivudine\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO NNRTI: either nevirapine or efavirenz based on availability (provided by national ART programmes in Uganda/Zimbabwe). Nevirapine syrup or tablet dosed twice-daily according to weight-bands following WHO. Efavirenz tablets dosed once-daily according to weight-bands following WHO.

Drug: Arm B: ZDV+ABC+3TC+NNRTI->ABC+3TC+NNRTI maintenance

Arm C: ZDV+ABC+3TC+NNRTI->ZDV+ABC+3TC maintenance

EXPERIMENTAL

ZDV \[abacavir\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO ABC \[abacavir\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO 3TC \[lamivudine\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO NNRTI: either nevirapine or efavirenz based on availability (provided by national ART programmes in Uganda/Zimbabwe). Nevirapine syrup or tablet dosed twice-daily according to weight-bands following WHO. Efavirenz tablets dosed once-daily according to weight-bands following WHO.

Drug: Arm C: ZDV+ABC+3TC+NNRTI->ZDV+ABC+3TC maintenance

Once-daily ABC+3TC

EXPERIMENTAL

ABC \[abacavir\]: syrup or tablet, dosed once-daily according to weight-bands following WHO 3TC \[lamivudine\]: syrup or tablet, dosed once-daily according to weight-bands following WHO

Drug: Once-daily ABC+3TC

Twice-daily ABC+3TC

ACTIVE COMPARATOR

ABC \[abacavir\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO 3TC \[lamivudine\]: syrup or tablet, dosed twice-daily according to weight-bands following WHO

Drug: Twice-daily ABC+3TC

Continued cotrimoxazole prophylaxis

ACTIVE COMPARATOR

Once-daily doses 5-\<15 kg: 200 mg of trimethoprim + 40 mg sulfamethoxazole 15-\<30 kg: 400 mg trimethoprim + 80 mg sulfamethoxazole \>=30 kg: 800 mg trimethoprim + 160 mg sulfamethoxazole

Drug: Continued cotrimoxazole prophylaxis

Stopped cotrimoxazole prophylaxis

EXPERIMENTAL

Children had been taking once-daily cotrimoxazole prophylaxis since at least ART initiation. Children randomised to this experimental arm stopped taking cotrimoxazole prophylaxis.

Other: Stopped cotrimoxazole prophylaxis

Interventions

Participants were examined by a doctor and had routine full blood count with white cell differential, lymphocyte subsets (CD4, CD8), biochemistry tests (bilirubin, urea, creatinine, aspartate aminotransferase, alanine aminotransferase) at screening, randomisation (lymphocytes only), weeks 4, 8, and 12, then every 12 weeks. Screening results were used to assess eligibility. All subsequent results at and after randomisation were only returned if requested for clinical management (authorised by centre project leaders); haemoglobin results at week 8 were automatically returned on the basis of early anaemia in a previous adult trial as were grade 4 laboratory toxicities (protocol safety criteria). Total lymphocytes and CD4 tests were never returned for CDM participants, but for all children other investigations (including tests from the routine panels) could be requested and concomitant drugs prescribed, as clinically indicated at extra patient-initiated or scheduled visits.

Clinically Driven Monitoring (CDM)

Participants were examined by a doctor and had routine full blood count with white cell differential, lymphocyte subsets (CD4, CD8), biochemistry tests (bilirubin, urea, creatinine, aspartate aminotransferase, alanine aminotransferase) at screening, randomisation (lymphocytes only), weeks 4, 8, and 12, then every 12 weeks. All results were returned to physicians for patient management. Other investigations (including tests from the routine panels) could be requested and concomitant drugs prescribed, as clinically indicated at extra patient-initiated or scheduled visits.

Laboratory plus Clinical Monitoring (LCM)

Children received a standard WHO-recommended regimen of open-label lamivudine, abacavir, plus NNRTI continuously. The NNRTI (nevirapine or efavirenz) was chosen by clinicians according to local availability and age.

Also known as: ABC: abacavir: Ziagen, 3TC: lamivudine: Epivir, ABC+3TC co-formulated: Kivexa, NVP: nevirapine, Viramune, EFV: efavirenz, Sustiva
Arm A: abacavir (ABC)+lamivudine (3TC)+NNRTI

Children initiated ART using an induction-maintenance approach, starting with open-label four-drug lamivudine, abacavir, NNRTI, plus zidovudine for 36 weeks, then open-label lamivudine, abacavir, plus NNRTI subsequently. The NNRTI (nevirapine or efavirenz) was chosen by clinicians according to local availability and age.

Also known as: ZDV: zidovudine, azidothymidine, Retrovir, ABC: abacavir: Ziagen, 3TC: lamivudine: Epivir, ZDV+3TC co-formulated: Combivir, ABC+3TC co-formulated: Kivexa, ZDV+ABC+3TC co-formulated: Trizivir, NVP: nevirapine, Viramune, EFV: efavirenz, Sustiva
Arm B: ZDV+ABC+3TC+NNRTI->ABC+3TC+NNRTI maintenance

Children initiated ART using an induction-maintenance approach, starting with open-label four-drug lamivudine, abacavir, NNRTI, plus zidovudine for 36 weeks, then open-label lamivudine, abacavir, plus zidovudine subsequently (triple NRTI maintenance). The NNRTI (nevirapine or efavirenz) was chosen by clinicians according to local availability and age.

Also known as: ZDV: zidovudine, azidothymidine, Retrovir, ABC: abacavir: Ziagen, 3TC: lamivudine: Epivir, ZDV+3TC co-formulated: Combivir, ABC+3TC co-formulated: Kivexa, ZDV+ABC+3TC co-formulated: Trizivir, NVP: nevirapine, Viramune, EFV: efavirenz, Sustiva
Arm C: ZDV+ABC+3TC+NNRTI->ZDV+ABC+3TC maintenance
Also known as: ABC: abacavir: Ziagen, 3TC: lamivudine: Epivir, ABC+3TC co-formulated: Kivexa
Once-daily ABC+3TC
Also known as: ABC: abacavir: Ziagen, 3TC: lamivudine: Epivir, ABC+3TC co-formulated: Kivexa
Twice-daily ABC+3TC
Also known as: trimethoprim+sulfamethoxazole
Continued cotrimoxazole prophylaxis
Stopped cotrimoxazole prophylaxis

Eligibility Criteria

Age3 Months - 17 Years
Sexall
Healthy VolunteersNo
Age GroupsChild (0-17)

You may qualify if:

  • Children should have an adult carer in the household who is either:
  • participating in the DART trial OR
  • being treated with ART OR
  • HIV positive but not yet needing treatment but with access to a treatment programme when ART is required OR
  • HIV negative. Children of DART participants should have first priority on any available remaining slots to enter ARROW.
  • Parents or guardians, and children where appropriate according to age and knowledge of HIV status, must be willing and able to give informed consent for randomisation to CDM or LCM and to first-line ART strategy.
  • Participants must have a confirmed documented diagnosis of HIV-1 infection:
  • For children aged under 18 months: two separate peripheral blood specimens from different days, both results being positive with HIV-DNA polymerase chain reaction (PCR).
  • For children aged 18 months or over: antibody positive serology by ELISA test (confirmed by licensed second ELISA or Western Blot) or WHO approved rapid test (performed in series) both on the same sample. Any child previously tested at another clinic should have a repeat test at an ARROW screening laboratory to confirm their status.
  • Age 3 months to 17 years (13-17 years to be capped at 10%)
  • ART naïve (except for exposure to perinatal ART for the prevention of mother-to-child HIV transmission).
  • Meeting criteria for requiring ART according to WHO stage and CD4 percent or count:
  • WHO paediatric clinical stage IV disease: treat regardless of CD4 percent or count
  • WHO paediatric clinical stage III disease:
  • \<12 months: treat all
  • +6 more criteria

You may not qualify if:

  • Cannot, or unlikely to attend regularly (e.g. usual residence too far from study centre)
  • Likelihood of poor adherence
  • Presence of acute infection (e.g. malaria, helminthiasis, acute hepatitis, acute pneumonia, septicaemia, meningitis). Children may be admitted after recovery of an acute infection. Children with chronic lung disease, including recurrent respiratory infections, are eligible. Children with tuberculosis (TB) will not be enrolled while on the intensive phase of anti-tuberculosis therapy, but should be re-evaluated after the intensive phase and a decision made then about starting ART (see 4 below)
  • In receipt of medication contraindicated by ART
  • children under three years of age receiving anti-tuberculosis therapy should not be enrolled (as they will have to receive nevirapine).
  • on chemotherapy for malignancy
  • Laboratory abnormalities which are a contra-indication for the child to start ART (haemoglobin \<8.5g/dL; neutrophils \<0.50x109/L; aspartate transaminase (AST) or alanine transaminase (ALT) \>5 x the upper limit of normal (ULN); grade 3 renal dysfunction - creatinine \>1.9 x ULN).
  • N.B. causes of anaemia, such as concurrent bacterial infection, malaria, helminthiasis and/or malnutrition should be investigated, and treatment for anaemia and its causes commenced prior to re-screening for eligibility.
  • Being pregnant or breast-feeding an infant
  • Perinatal exposure to nevirapine (either through prevention of mother-to-child transmission (pMTCT) or breastfeeding) for children aged 3 - 6 months only
  • Participating in ARROW
  • On ART for at least 36 weeks
  • Currently taking lamivudine+abacavir twice daily as part of their ART regimen and expected to stay on these two drugs for at least the next 12 weeks
  • Parents or guardians, and children where appropriate according to age and knowledge of HIV status, must be willing and able to give informed consent for randomisation to once or twice daily lamivudine+abacavir
  • Likely to switch to second-line therapy in the next 12 weeks
  • +7 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (4)

MRC /UVRI Uganda Research Unit on AIDS

Entebbe, Uganda

Location

Joint Clinical Research Centre

Kampala, Uganda

Location

Baylor College of Medicine Children's Foundation

Mulago, Uganda

Location

University of Zimbabwe Medical School

Harare, Zimbabwe

Location

Related Publications (3)

  • ARROW Trial team. Routine versus clinically driven laboratory monitoring and first-line antiretroviral therapy strategies in African children with HIV (ARROW): a 5-year open-label randomised factorial trial. Lancet. 2013 Apr 20;381(9875):1391-1403. doi: 10.1016/S0140-6736(12)62198-9. Epub 2013 Mar 7.

  • Bwakura-Dangarembizi M, Kendall L, Bakeera-Kitaka S, Nahirya-Ntege P, Keishanyu R, Nathoo K, Spyer MJ, Kekitiinwa A, Lutaakome J, Mhute T, Kasirye P, Munderi P, Musiime V, Gibb DM, Walker AS, Prendergast AJ. A randomized trial of prolonged co-trimoxazole in HIV-infected children in Africa. N Engl J Med. 2014 Jan 2;370(1):41-53. doi: 10.1056/NEJMoa1214901.

  • Musiime V, Kasirye P, Naidoo-James B, Nahirya-Ntege P, Mhute T, Cook A, Mugarura L, Munjoma M, Thoofer NK, Ndashimye E, Nankya I, Spyer MJ, Thomason MJ, Snowden W, Gibb DM, Walker AS; ARROW Trial Team. Once vs twice-daily abacavir and lamivudine in African children. AIDS. 2016 Jul 17;30(11):1761-70. doi: 10.1097/QAD.0000000000001116.

Related Links

MeSH Terms

Conditions

Acquired Immunodeficiency Syndrome

Interventions

LaboratoriesNevirapineefavirenzZidovudineTrimethoprim, Sulfamethoxazole Drug Combination

Condition Hierarchy (Ancestors)

HIV InfectionsBlood-Borne InfectionsCommunicable DiseasesInfectionsSexually Transmitted Diseases, ViralSexually Transmitted DiseasesLentivirus InfectionsRetroviridae InfectionsRNA Virus InfectionsVirus DiseasesSlow Virus DiseasesGenital DiseasesUrogenital DiseasesImmunologic Deficiency SyndromesImmune System Diseases

Intervention Hierarchy (Ancestors)

Non-Medical Public and Private FacilitiesHealth FacilitiesHealth Care Facilities Workforce and ServicesPyridinesHeterocyclic Compounds, 1-RingHeterocyclic CompoundsThymidinePyrimidine NucleosidesPyrimidinesDideoxynucleosidesDeoxyribonucleosidesNucleosidesNucleic Acids, Nucleotides, and NucleosidesSulfamethoxazoleBenzenesulfonamidesSulfonamidesAmidesOrganic ChemicalsSulfanilamidesAniline CompoundsAminesBenzene DerivativesHydrocarbons, AromaticHydrocarbons, CyclicHydrocarbonsSulfonesSulfur CompoundsTrimethoprimDrug CombinationsPharmaceutical Preparations

Results Point of Contact

Title
Professor Ann Sarah Walker
Organization
Medical Research Council

Study Officials

  • Diana M Gibb, MD

    Medical Research Council

    PRINCIPAL INVESTIGATOR
  • Peter Mugyenyi, PhD

    Joint Clinical Research Centre, Kampala, Uganda

    PRINCIPAL INVESTIGATOR
  • Kusum Nathoo, PhD

    University of Zimbabwe, Harare, Zimbabwe

    PRINCIPAL INVESTIGATOR
  • Adeodata Kekitiinwa, MD

    Baylor College of Medicine Children's Foundation, Mulago, Uganda

    PRINCIPAL INVESTIGATOR
  • Paula Munderi, MBChB

    MRC /UVRI Uganda Research Unit on AIDS, Entebbe, Uganda

    PRINCIPAL INVESTIGATOR
  • Victor Musiime, PhD

    Joint Clinical Research Centre, Kampala, Uganda

    PRINCIPAL INVESTIGATOR
  • Mutsa F Bwakura-Dangarembizi, MBChB

    University of Zimbabwe, Harare, Zimbabwe

    PRINCIPAL INVESTIGATOR
  • Philippa Musoke, PhD

    Baylor College of Medicine Children's Foundation, Mulago, Uganda

    PRINCIPAL INVESTIGATOR
  • Sabrina Bakeera-Kitaka, MBChB

    Baylor College of Medicine Children's Foundation, Mulago, Uganda

    PRINCIPAL INVESTIGATOR
  • Patricia Nahirya-Ntege, MBChB

    MRC/UVRI and LSHTM Uganda Research Unit

    PRINCIPAL INVESTIGATOR

Publication Agreements

PI is Sponsor Employee
No
Restrictive Agreement
No

Study Design

Study Type
interventional
Phase
phase 4
Allocation
RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
FACTORIAL
Sponsor Type
OTHER GOV
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Professor of Epidemiology

Study Record Dates

First Submitted

December 31, 2013

First Posted

January 7, 2014

Study Start

March 1, 2007

Primary Completion

March 1, 2012

Study Completion

June 1, 2012

Last Updated

June 6, 2014

Results First Posted

June 6, 2014

Record last verified: 2014-06

Locations