Non- Essential Amino Acid Requirements and Metabolism in Humans

NCT02009917 | Completed DMC

• 1 other identifier: H12-01683
• Study Type: observational
• Target: 6
• Locations: 1 country
• Sites: 1

Brief Summary

Protein is a major structural component of all cells in the body. The nutritional importance of protein is because of their amino acids. 9 are called essential and 11 are called non-essential, based on whether we need to get them from diet. This classification is based on studies done using older techniques. Recent studies suggest that these so called non-essential amino acids play important roles in our body's health. For the first time, this proposal defines an experimental design to examine non- essential amino acid/nitrogen requirements and metabolism in humans using the Indicator Amino Acid Oxidation (IAAO) technique. The objective of the current study is to examine the application of IAAO technique to determine the non-essential amino acid/nitrogen requirement in adult humans.

Conditions

Healthy Adult Males

Keywords

Indicator Amino Acid Oxidation; Non essential Amino acids; Protein; Amino acid

Outcome Measures

Primary Outcomes (1)

• 13 Carbon dioxide production

  Urine and breath samples will be collected to measure the rate of oxidation of expired breath and flux by enrichment of urine.

  8 hours (1study day)

Eligibility Criteria

• Age: 20 Years - 40 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

Physiologically Healthy Adult males, 20-40 years of age

You may qualify if:

• years of age
• Male
• Classified as normal body weight based on body mass index (BMI) 18.5-25kg/m2
• Free of any concurrent illness

You may not qualify if:

• Men not in good health or have a metabolic, neurological, genetic or immune disorder including diabetes and hypertension
• Men who are classified as underweight(\<18.5kg/m2), overweight(25-30kg/m2) or obese (\>30kg/m2) using the BMI classification)
• Men who are allergic to milk, eggs and egg protein

Sponsors & Collaborators

• University of British Columbia: lead
• Ajinomoto Co., Inc.: collaborator

Study Sites (1)

Child and Family Research Institute

Vancouver, British Columbia, V5Z4H4, Canada

Location

Related Publications (1)

• Cooper L, Ball RO, Pencharz PB, Sakai R, Elango R. Dispensable Amino Acids, except Glutamine and Proline, Are Ideal Nitrogen Sources for Protein Synthesis in the Presence of Adequate Indispensable Amino Acids in Adult Men. J Nutr. 2020 Sep 1;150(9):2398-2404. doi: 10.1093/jn/nxaa180.

  PMID: 32879983 DERIVED

Biospecimen

Retention: SAMPLES WITHOUT DNA

Breath and urine samples

Study Officials

• Rajavel Elango, Ph.D

  University of British Columbia

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principle Investigator

Study Record Dates

• First Submitted: December 9, 2013
• First Posted: December 12, 2013
• Study Start: October 1, 2013
• Primary Completion: January 1, 2015
• Study Completion: April 1, 2015
• Last Updated: July 18, 2017

Record last verified: 2017-07

Locations

CA (1)