NCT01922024

Brief Summary

The Curetis Unyvero LRT55 Application is intended to detect and to identify genes of 21 microorganisms and 19 genes associated with antibiotic resistance in 4 hours. In this study, the performance of the Unyvero LRT55 Application shall be tested under clinical conditions and compared to (1) a composite reference method (for non-atypical or cultured microorganisms) or (2) a molecular PCR based reference method for the 3 atypical microorganisms Chlamydophila pneumoniae, Legionella pneumoniae, and Pneumocystis jirovecii, and for resistance genes. PCR amplifications are followed by bi-directional sequencing, including comparison of the test results Time to result will be compared for the Unyvero LRT55 Application and standard-of care.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
2,124

participants targeted

Target at P75+ for all trials

Timeline
Completed

Started Jun 2015

Geographic Reach
1 country

9 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

August 7, 2013

Completed
7 days until next milestone

First Posted

Study publicly available on registry

August 14, 2013

Completed
1.8 years until next milestone

Study Start

First participant enrolled

June 1, 2015

Completed
1.4 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

November 1, 2016

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

November 1, 2016

Completed
2.3 years until next milestone

Results Posted

Study results publicly available

March 5, 2019

Completed
Last Updated

March 5, 2019

Status Verified

February 1, 2019

Enrollment Period

1.4 years

First QC Date

August 7, 2013

Results QC Date

January 25, 2017

Last Update Submit

February 28, 2019

Conditions

Outcome Measures

Primary Outcomes (1)

  • Clinical Sensitivity and Specificity for Microorganism Detection as Compared to Routine Microbiology and Other Reference Methods Including PCR and Sequencing

    Sensitivity for microorganism detection will be determined against the respective reference method consisting of standard procedure and/or a reference method based on PCR and bi-directional sequencing using alternative primers. Sensitivity for non-atypical microorganism detection will thus be determined if at least 1 out of 2 reference methods have identified microorganisms that are covered by the panel of the investigational IVD. Results will further be analyzed against standard of-care alone, as well as against composite comparator. Sensitivity for atypical microorganism detection will be determined against PCR with alternative primers as reference method. Specificity for microorganism detection will be determined in all samples included in the trial. Individual specificities will be calculated for the detection of the respiratory microorganisms analyzed by the investigational IVD compared to the microorganisms reference method.

    Up to 12 months

Study Arms (2)

Prospective Fresh Clinical Specimens

Patients with suspicion of lower respiratory tract infection (pneumonia), samples tested with Unyvero LRT 55 cartridge and results compared against composite (comparator) which is based on routine clinical microbiology and a second PCR test

Device: LRT55 Testing

Retrospective Frozen Clinical Specimens

Patients with suspicion of lower respiratory tract infection (pneumonia), samples tested with Unyvero LRT 55 cartridge and results compared against routine clinical microbiology

Device: LRT55 Testing

Interventions

Testing on the Unyvero LRT55

Prospective Fresh Clinical SpecimensRetrospective Frozen Clinical Specimens

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Suspicion of lower respiratory tract infection in hospitalized subjects.

You may qualify if:

  • Hospitalized subjects with suspicion of lower respiratory tract infection
  • Age at least 18 years
  • Available surplus respiratory aspirate or bronchial lavage sample

You may not qualify if:

  • Out-patient (ambulatory patient)
  • Known infection with HIV, HBV or tuberculosis

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (9)

University of California Los Angeles (UCLA)

Los Angeles, California, 90049, United States

Location

Northwestern Memorial Hospital

Chicago, Illinois, 60611, United States

Location

Johns Hopkins Hospital

Baltimore, Maryland, 21287, United States

Location

William Beaumont Hospital

Royal Oak, Michigan, 48073, United States

Location

Mayo Clinic

Rochester, Minnesota, 55901, United States

Location

Columbia Universiy Medical Center / New York-Presbyterian Hosp.

New York, New York, 10032, United States

Location

Rochester Medical Center

Rochester, New York, 14642, United States

Location

Summa Health System

Akron, Ohio, 44304, United States

Location

University of Washington

Seattle, Washington, 98195, United States

Location

Biospecimen

Retention: SAMPLES WITH DNA

Bronchial lavage (BAL) Tracheal aspirate (TA)

MeSH Terms

Conditions

Pneumonia

Condition Hierarchy (Ancestors)

Respiratory Tract InfectionsInfectionsLung DiseasesRespiratory Tract Diseases

Results Point of Contact

Title
Clinical Operations
Organization
Curetis USA, Inc.

Study Officials

  • Johannes Bacher

    Curetis GmbH

    STUDY DIRECTOR

Publication Agreements

PI is Sponsor Employee
No
Restrictive Agreement
No

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

August 7, 2013

First Posted

August 14, 2013

Study Start

June 1, 2015

Primary Completion

November 1, 2016

Study Completion

November 1, 2016

Last Updated

March 5, 2019

Results First Posted

March 5, 2019

Record last verified: 2019-02

Locations