NCT01893710

Brief Summary

Within few years the peritoneal membrane of adult peritoneal dialysis (PD) patients undergoes substantial morphological transformation, including progressive fibrosis, vasculopathy and neoangiogenesis. Ultrafiltration capacity steadily declines and ultimately results in PD failure. In children, peritoneal biopsies demonstrating PD associated alterations have not yet been obtained. They, however, should be particularly informative, since secondary tissue and vascular pathology related to ageing or diabetes is absent. An international, prospective peritoneal membrane biopsy study in children on PD will therefore be performed. Biopsies will be obtained at time of PD catheter insertion, on occasion of intercurrent abdominal surgery (e.g. hernia repair, catheter exchange) and at time of renal transplantation. Quantitative histomorphometry and tissue protein expression analyses will be correlated with time integrated PD treatment modalities and functional characteristics as well as inflammatory and cardiovascular comorbidity surrogate parameter. Blood will be obtained during clinical routine sampling. Biopsies will be obtained during clinically indicated operations, without substantially increasing operation time and associated surgical risks. The detailed histomorphometry of the PD membrane will give additional information, potentially impacting on the individual PD regime. 3/2018: The analyses of the pediatric PD biopsy demonstrated early and major transformation of the peritoneal membrane with neutral pH low GDP fluids, and significant vasculopathy already in children with CKD stage 5, further progressing with PD. The underlying mechanisms are partly understood, only. In view of these major findings and the numerous open questions, collection of biosamples will be continued in children and also in adult PD patients. The following questions will be addressed: Molecular counterparts of peritoneal semi-permeability, solute and water transport (beyond AQP1), pathomechanisms and molecular and functional impact of peritoneal transformation with low and high GDP fluids, and the respective pathomechanisms and molecular and functional impact of vascular disease in CKD and with different PD fluids. The impact of renal transplantation following PD will be assessed in a subgroup of patients with tenckhoff catheter removal several weeks after transplantation and a functioning graft.

Trial Health

88
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
500

participants targeted

Target at P75+ for all trials

Timeline
32mo left

Started Feb 2011

Longer than P75 for all trials

Geographic Reach
15 countries

26 active sites

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress85%
Feb 2011Dec 2028

Study Start

First participant enrolled

February 1, 2011

Completed
2.4 years until next milestone

First Submitted

Initial submission to the registry

June 27, 2013

Completed
12 days until next milestone

First Posted

Study publicly available on registry

July 9, 2013

Completed
15.2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

October 1, 2028

Expected
3 months until next milestone

Study Completion

Last participant's last visit for all outcomes

December 31, 2028

Last Updated

April 30, 2026

Status Verified

December 1, 2024

Enrollment Period

17.7 years

First QC Date

June 27, 2013

Last Update Submit

April 29, 2026

Conditions

Keywords

peritoneal dialysisparietal peritoneumomentumchronic kidney diseasevasculopathy

Outcome Measures

Primary Outcomes (1)

  • Peritoneal vasculopathy (lumen vessel ratio)

    Digital quantification of degree of vasculopathy, i.e the lumen vessel ratio. Healthy children have a L/V ratio of about 0.7. lower values represent vasculopathy with lumen narrowing, 0 is complete obliteration of the vessel. This measurements will be accompanied by molecular analysis of pathomechanisms (including omics Technology)

    Two years (Mean PD treatment time)

Secondary Outcomes (1)

  • Number of vessels per peritoneal membrane area (per mm²)

    at time of catheter insertion, intercurrent abdominal surgery and at time of renal transplantation

Other Outcomes (3)

  • Submesothelial thickness (µm)

    2 years (average PD duration)

  • Submesothelial lymphocyte, macrophage, MMT cell count

    2 years (mean PD duration)

  • Peritoneal VEGF and pSMAD abundance

    2 years (mean PD duration)

Study Arms (4)

Control

'Biopsy sampling': Peritoneal biopsies without kidney disease, i.e. diseases not related to the kidney and not affecting the peritoneum. This group is accomplished.

chronic kidney disease

Samples will obtained from patients with chronic kidney disease stage 5 (at time of catheter Insertion)

Procedure: biopsy sampling

Peritoneal dialysis

Patients on PD with different PD fluids and intercurrent abdominal surgery and at time of renal transplantation.

Procedure: biopsy sampling

Post PD and with functioning graft

Samples will also be collected and analysed from patients with renal transplantation after PD at time of and tenckhoff catheter removal several weeks after Tx or other intercurrent abdominal surgery.

Procedure: biopsy sampling

Interventions

Two parietal peritoneal samples, each 1 cm² x 0.3 cm in depth and three omental tissue samples, each 1 cm² in size will be obtained. Biopsy sampling will be performed in all groups. This is an observational not an interventional trial.

Peritoneal dialysisPost PD and with functioning graftchronic kidney disease

Eligibility Criteria

Age1 Day - 90 Years
Sexall
Healthy VolunteersYes
Age GroupsChild (0-17), Adult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

See eligibility data

You may qualify if:

  • Age 0 to 90 years
  • CKD 5D, peritoneal dialysis and
  • Patients with normal renal function and elective abdominal surgery due to limited abdominal pathology (such as hernia repair, gallstones….)
  • Patients post PD and post Tx
  • Oral and written consent
  • Ability to consent of the adult patient and of the parents and legal guardian of patients not yet of legal age, respectively

You may not qualify if:

  • Abdominal adhesions, malformation and inflammation beyond PD induced changes
  • Patients with disseminated tumour disease
  • Patients with critical heart failure and other medical conditions, where the additional procedure may confer an increased increase risk
  • Pregnancy
  • Preterm babies (below 37 weeks of gestational age)
  • Serum hemoglobin \< 10 g/dl in newborns and \< 8 g/dl in children and adults

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (26)

University of Alabama at Birmingham

Birmingham, Alabama, 35233, United States

ACTIVE NOT RECRUITING

Children's Mercy Hospital

Kansas City, Missouri, 64108, United States

RECRUITING

The Children´s Hospital of Philadelphia

Narberth, Pennsylvania, 19104, United States

ACTIVE NOT RECRUITING

Department of Pediatrics, Medical University Vienna

Vienna, 1090, Austria

RECRUITING

UZ Ghent

Ghent, 9890, Belgium

RECRUITING

University Children's Hospital

Prague, 15006, Czechia

RECRUITING

Service de Néphrologie Pédiatrique, Hôpital Femme Mere Enfant

Lyon, 69677, France

RECRUITING

University Children's Hospital

Strasbourg, 67098, France

RECRUITING

Department of Medicine I (Nephrology), University of Heidelberg

Heidelberg, Baden-Wurttemberg, 69120, Germany

RECRUITING

University Children's Hospital

Berlin, 10117, Germany

ACTIVE NOT RECRUITING

University Children's Hospital

Cologne, 50931, Germany

RECRUITING

University Children's Hospital

Essen, 45122, Germany

RECRUITING

UKE, University Children´s Hospital

Hamburg, 20251, Germany

RECRUITING

KfH Pediatric Kidney Center, Department of Pediatric Nephrology, University of Marburg

Marburg, 35043, Germany

RECRUITING

University Children's Hospital

Budapest, 1083, Hungary

RECRUITING

University Children'Hospital

Genova, 16147, Italy

ACTIVE NOT RECRUITING

University Children's Hospital

Milan, 20122, Italy

RECRUITING

Pediatric Nephrology, Dialysis and Transplant Unit

Padova, 35128, Italy

ACTIVE NOT RECRUITING

University children's Hospital

Vilnius, 08406, Lithuania

RECRUITING

Paediatric CAPD unit, Kuala Lumpur Hospital

Kuala Lumpur, 50586, Malaysia

ACTIVE NOT RECRUITING

Krakow, Jagiellonian University Medical College

Krakow, 30663, Poland

RECRUITING

Hospital Universitario Materno-Infantil Vall d' Hebron

Barcelona, 08035, Spain

RECRUITING

Karolinska University Hospital

Stockholm, 17176, Sweden

ACTIVE NOT RECRUITING

Children's Hospital, Inselspital, Bern University Hospital and University of Bern

Bern, 3010, Switzerland

ACTIVE NOT RECRUITING

University Children's Hospital

Adana, 01330, Turkey (Türkiye)

RECRUITING

Cerrahpasa School of Medicine

Istanbul, 34303, Turkey (Türkiye)

ACTIVE NOT RECRUITING

Related Publications (3)

  • Levai E, Marinovic I, Bartosova M, Zhang C, Schaefer B, Jenei H, Du Z, Drozdz D, Klaus G, Arbeiter K, Romero P, Schwenger V, Schwab C, Szabo AJ, Zarogiannis SG, Schmitt CP. Human peritoneal tight junction, transporter and channel expression in health and kidney failure, and associated solute transport. Sci Rep. 2023 Oct 13;13(1):17429. doi: 10.1038/s41598-023-44466-z.

  • Catar RA, Bartosova M, Kawka E, Chen L, Marinovic I, Zhang C, Zhao H, Wu D, Zickler D, Stadnik H, Karczewski M, Kamhieh-Milz J, Jorres A, Moll G, Schmitt CP, Witowski J. Angiogenic Role of Mesothelium-Derived Chemokine CXCL1 During Unfavorable Peritoneal Tissue Remodeling in Patients Receiving Peritoneal Dialysis as Renal Replacement Therapy. Front Immunol. 2022 Feb 4;13:821681. doi: 10.3389/fimmu.2022.821681. eCollection 2022.

  • Bartosova M, Zhang C, Schaefer B, Herzog R, Ridinger D, Damgov I, Levai E, Marinovic I, Eckert C, Romero P, Sallay P, Ujszaszi A, Unterwurzacher M, Wagner A, Hildenbrand G, Warady BA, Schaefer F, Zarogiannis SG, Kratochwill K, Schmitt CP. Glucose Derivative Induced Vasculopathy in Children on Chronic Peritoneal Dialysis. Circ Res. 2021 Aug 20;129(5):e102-e118. doi: 10.1161/CIRCRESAHA.121.319310. Epub 2021 Jul 8.

Related Links

Biospecimen

Retention: SAMPLES WITH DNA

Parietal ond omental peritoneal membrane specimen will be collected in all groups

MeSH Terms

Conditions

Kidney Failure, ChronicRenal Insufficiency, ChronicVascular Diseases

Interventions

Chorionic Villi Sampling

Condition Hierarchy (Ancestors)

Renal InsufficiencyKidney DiseasesUrologic DiseasesFemale Urogenital DiseasesFemale Urogenital Diseases and Pregnancy ComplicationsUrogenital DiseasesMale Urogenital DiseasesChronic DiseaseDisease AttributesPathologic ProcessesPathological Conditions, Signs and SymptomsCardiovascular Diseases

Intervention Hierarchy (Ancestors)

BiopsyCytodiagnosisCytological TechniquesClinical Laboratory TechniquesDiagnostic Techniques and ProceduresDiagnosisSpecimen HandlingPrenatal DiagnosisDiagnostic Techniques, Obstetrical and GynecologicalDiagnostic Techniques, SurgicalSurgical Procedures, OperativeInvestigative Techniques

Study Officials

  • Claus P Schmitt, MD

    University of Heidelberg, Center for Pediatric and Adolescent Medicine

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Study Design

Study Type
observational
Observational Model
OTHER
Time Perspective
PROSPECTIVE
Target Duration
2 Years
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
MD

Study Record Dates

First Submitted

June 27, 2013

First Posted

July 9, 2013

Study Start

February 1, 2011

Primary Completion (Estimated)

October 1, 2028

Study Completion (Estimated)

December 31, 2028

Last Updated

April 30, 2026

Record last verified: 2024-12

Locations