Swim up and Gradient Methods Used in Assisted Reproduction Techniques on DNA Fragmentation of Spermatozoa

NCT01859520 | Completed

• 1 other identifier: Gazi University 01/2011-110
• Study Type: interventional
• Target: 65
• Locations: 0 countries
• Sites: N/A

Brief Summary

Sperms are prepared by sperm washing techniques before used in assisted reproductive technologies. Most commonly used sperm preparation methods are the swim up and the density gradient. Recent studies shows that the DNA integrity status of the spermatozoa is related to the success in assisted reproduction techniques. Sperm preparation methods may theoretically cause damage to sperm DNA. Therefore it is important to select the optimum method of sperm preparation causing least sperm DNA damage. Aim of our study is to investigate and compare the effect two different sperm preparation techniques on DNA fragmentation.

Conditions

Male Infertility; Unexplained Infertility

Keywords

Spermatozoa; DNA fragmentation; Swim-up; Density gradient

Outcome Measures

Primary Outcomes (1)

• The change in the rate of DNA fragmentation of spermatozoa after sperm preparation with either swim-up or density gradient techniques.

  The change in the rate of DNA fragmented spermatozoa will be assessed before and immediately after the sperm preparation is completed, because DNA fragmentation of spermatozoa may be increased in time.

  DNA fragmentation assay is performed just before and immediately after the sperm preparation is completed. The time frame is within an hour.

Study Arms (1)

Swim up, density gradient

EXPERIMENTAL

swim-up and density gradient sperm preparation techniques in male factor infertility and unexplained infertility groups

Procedure: swim-up, density gradient sperm preparation techniques

Interventions

swim-up, density gradient sperm preparation techniques PROCEDURE

Sperms are prepared by sperm washing techniques before used in assisted reproductive technologies. Most commonly used sperm preparation methods are the swim up and the density gradient. Recent studies shows that the DNA integrity status of the spermatozoa is related to the success in assisted reproduction techniques. Sperm preparation methods may theoretically cause damage sperm DNA. Therefore it is important to select the optimum method of sperm preparation causing least sperm DNA damage. Aim of our study is to investigate and compare the effect two different sperm preparation techniques on DNA fragmentation.

Swim up, density gradient

Eligibility Criteria

• Age: 20 Years - 45 Years
• Sex: male
• Healthy Volunteers: No
• Age Groups: Adult (18-64)

You may qualify if:

• Male factor subfertile group:
• Couples of having at least two abnormal sperm analysis according to WHO criteria.
• Unexplained subfertile group:
• Couples with normal sperm parameters, plus females with normal ovulatory status and patent fallopian tubes by hysterosalpingography or laparoscopy.
• No previous IVF or IUI attempts before the study

You may not qualify if:

• Severe oligospermia (Sperm count \< 5 Millions/ per ml) Systemic diseases or therapies influencing DNA integrity for male partner

Sponsors & Collaborators

• Gazi University: lead

MeSH Terms

Conditions

Infertility, Male

Condition Hierarchy (Ancestors)

Genital Diseases, Male; Genital Diseases; Urogenital Diseases; Infertility; Male Urogenital Diseases

Study Officials

• Ahmet erdem, Professsor, MD

  University of Gazi School of Medicine Department of Obstetrics and Gynecology

  STUDY DIRECTOR

• Yuksel Oguz, Resident, MD

  University of Gazi School Of Medicine Department of Obstetrics and Gynecology

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: DIAGNOSTIC
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Professor of Obstetrics and Gynecology

Study Record Dates

• First Submitted: March 25, 2013
• First Posted: May 22, 2013
• Study Start: July 1, 2012
• Primary Completion: October 1, 2012
• Study Completion: December 1, 2012
• Last Updated: May 22, 2013

Record last verified: 2013-05