NCT01745523

Brief Summary

This study is aimed to evaluate the use of Hydroxypropyl Cellulose (HPC) as substitute for the traditional protein supplement (Synthetic Serum Substitute; SSS) and Trehalose as substitute for the most widely used sugar (Sucrose) in the vitrification solutions employed for oocyte vitrification.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
440

participants targeted

Target at P75+ for not_applicable

Timeline
Completed

Started Nov 2012

Typical duration for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Start

First participant enrolled

November 1, 2012

Completed
28 days until next milestone

First Submitted

Initial submission to the registry

November 29, 2012

Completed
11 days until next milestone

First Posted

Study publicly available on registry

December 10, 2012

Completed
1.3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 1, 2014

Completed
6 months until next milestone

Study Completion

Last participant's last visit for all outcomes

October 1, 2014

Completed
Last Updated

March 10, 2015

Status Verified

March 1, 2015

Enrollment Period

1.4 years

First QC Date

November 29, 2012

Last Update Submit

March 9, 2015

Conditions

Keywords

Oocyte vitrificationsurvival rateegg bankingovum donation

Outcome Measures

Primary Outcomes (1)

  • Survival after oocyte vitrification using cryoprotective solutions containing hiroxipropilcelulosa (HPC) and trehalose.

    After warming and embryo transfer. Survival will be evaluated morphologically two hours after warming.

    >2 hours

Secondary Outcomes (1)

  • Embryo development

    From time of thawing until pregnancy outcome (0-9 months)

Study Arms (2)

Vitrified oocytes using HPC+Trehalose

EXPERIMENTAL

Oocytes are vitrified using the synthetic macromolecule HPC and trehalose

Procedure: Synthetic macromolecule HPC and trehalose

Vitrified oocytes using SSS+ Sucrose

ACTIVE COMPARATOR

Oocytes are vitrified using the SSS containing HSA and sucrose

Procedure: SSS containing HSA and sucrose

Interventions

Oocytes are vitrified using the synthetic macromolecule HPC and trehalose

Vitrified oocytes using HPC+Trehalose

• Oocytes are vitrified using the SSS containing HSA and sucrose

Vitrified oocytes using SSS+ Sucrose

Eligibility Criteria

Age18 Years - 50 Years
Sexfemale
Healthy VolunteersNo
Age GroupsAdult (18-64)

You may qualify if:

  • Donors:
  • \< 35 years old
  • Normal physical and gynecological examinations
  • No family history of hereditary or chromosomal diseases.
  • Normal karyotype
  • Negative screening for sexually transmitted diseases.
  • Oocyte recipients:
  • Oocyte recipients \< 50 years old
  • Body mass index\< 30
  • \< 2 previous IVF failures
  • No severe male factor
  • No recurrent miscarriage
  • No hidrosalpinx
  • No myoma
  • No adenomyosis
  • +1 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

IVI Valencia

Valencia, 46117, Spain

Location

MeSH Terms

Conditions

Infertility

Interventions

TrehaloseSucrose

Condition Hierarchy (Ancestors)

Genital DiseasesUrogenital Diseases

Intervention Hierarchy (Ancestors)

GlucansPolysaccharidesCarbohydratesDisaccharidesOligosaccharidesSugars

Study Officials

  • Ana Cobo, PhD

    IVI Valencia

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
QUADRUPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Cryobiology Unit Director at IVI Valencia

Study Record Dates

First Submitted

November 29, 2012

First Posted

December 10, 2012

Study Start

November 1, 2012

Primary Completion

April 1, 2014

Study Completion

October 1, 2014

Last Updated

March 10, 2015

Record last verified: 2015-03

Locations