IL8 Monitoring and Its Correlation With 251-gene Polymorphism

NCT01708759 | Completed DMC

• 1 other identifier: 1380/09/12
• Study Type: observational
• Target: 80
• Locations: 1 country
• Sites: 1

Brief Summary

One hundred eighty critically ill African adult intensive care patients divided into two groups, eighty septic critically ill patients (sepsis group) while, eighty non-septic critically ill patients (SIRS) group. Admission serum IL-8 was measured in both sepsis and SIRS groups. IL-8 (-251A/T) polymorphism was detected in sepsis and SIRS groups.

Conditions

Sepsis; Systemic Inflammatory Response Syndrome

Keywords

Serum Il-8; IL8 polymorphism; critically ill patients

Outcome Measures

Primary Outcomes (1)

• Serum Interlukin-8

  Serum level of IL-8 was determined by quantitative sandwich enzyme immunoassay (R\&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer's instructions. The intensity of the colour was measured at 490 nm for IL-8.

  At intensive care admission. Patients will be followed for at least 2 weeks

Secondary Outcomes (1)

• Interlukine-8polymorphism at 251 A/t

  At ICU admission. Patients will be followed for at least 2weeks.

Study Arms (2)

Sepsis

Forty patients developed septic complication during ICU stay (sepsis group).

SIRS

Forty patients were critically ill without evidence of infectious organism (SIRS group).

Eligibility Criteria

• Age: 18 Years - 60 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64)
• Sampling Method: Probability Sample

Study Population

A total of eighty African patients (47men and 33 women) were included in the study. Forty patients developed septic complication during ICU stay (sepsis group). Forty patients were critically ill without evidence of infectious organism (SIRS group).

You may qualify if:

• Adults patients with clinical diagnosis of sepsis or systemic inflammatory response syndrome.

You may not qualify if:

• Patients received anti-inflammatory drugs or corticosteroids before admission, patients who had immunosuppressive illness ie. AIDS, patients with chronic liver failure, patients with renal failure, patients with respiratory failure ; patients received massive blood transfusion; patients with radiation therapy, patients with organ transplantation .

Sponsors & Collaborators

• Tanta University: lead

Study Sites (1)

Faculty of Medicine ,Tanta University.

Tanta, Algharbiya, 35217, Egypt

Location

Related Publications (1)

• Sutherland A M, Russell J A. Issues with polymorphism analysis is sepsis. Clinical infectious diseases 2005; 41; S369-402. Winning J, Claus RA, Huse K, Bauer M. Molecular biology on the ICU from understanding to treating sepsis. Minerva Anestesiol 2006; 72:255-67. Hull J, Thomson A, Kwiatkowski D:Association of respiratory syncytial virus broncholitis with the interleukin-8 gene region in UK families. Thorax 2000, 55: 1023-1027. Gyulai Z, Klausz G, Tiszai A, Lenart Z, Kasa IT, Lonovics J, Mandi Y. Genetic polymorphism of interleukin-8 (IL-8) is associated with Helicobacter pylori induced duodenal ulcer. Eur Cytokine Netw 2004, 15:353-358.

  BACKGROUND

MeSH Terms

Conditions

Sepsis; Systemic Inflammatory Response Syndrome

Condition Hierarchy (Ancestors)

Infections; Inflammation; Pathologic Processes; Pathological Conditions, Signs and Symptoms; Shock

Study Officials

• Ayman A Yousef, MD

  Assistant professor os Anesthesia and ICU, Faculty of Medicine, Tanta university

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant professor of Anesthesiology

Study Record Dates

• First Submitted: October 12, 2012
• First Posted: October 17, 2012
• Study Start: November 1, 2012
• Primary Completion: February 1, 2013
• Study Completion: February 1, 2013
• Last Updated: April 8, 2014

Record last verified: 2014-04

Locations

EG (1)