Obesity-related Genes in Taiwanese Undergoing Weight Loss

NCT01684280 | Completed DMC

• 1 other identifier: CRC-06-09-10
• Study Type: observational
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

To investigate:

1. 1.Associations between miRNA and insulin signaling-related gene expressions in abdominal adipose tissues in obese subjects.
2. 2.Differences in miRNAs expressed by intrabdominal omental adipose tissues between genders.

Conditions

Morbid Obesity

Keywords

abdominal adipose tissue; microRNA

Outcome Measures

Primary Outcomes (1)

• MicroRNA expression in abdominal omental adipose tissues

  MicroRNA-125a-3p expression in abdominal omental adipose tissues is associated with insulin signaling-related gene expression levels in morbid obesity

  up to 28 weeks

Study Arms (1)

gender

Paired samples of abdominal subcutaneous and intrabdominal omental adipose tissue were obtained from men and women who underwent bariatric surgery.

Genetic: gender

Interventions

gender GENETIC

Paired samples of abdominal subcutaneous and intrabdominal omental adipose tissue were obtained from men and women who underwent bariatric surgery.

Also known as: BMI were all over 40 kg/ m2

gender

Eligibility Criteria

• Age: 19 Years - 63 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64)
• Sampling Method: Probability Sample

Study Population

Paired samples of abdominal subcutaneous and intrabdominal omental adipose tissue were obtained from men (n = 9) and women (n =10) who underwent bariatric surgery. Their BMI were all over 40 kg/ m2, and ages between 19-63 years.

You may qualify if:

• Morbid obesity,BMIs\> 40 kg/m2
• Subjects who underwent bariatric surgery

You may not qualify if:

• Obese subjects with complicated metabolic disorders
• Thyroid disease

Sponsors & Collaborators

• Sung Ling Yeh: lead
• National Science and Technology Council, Taiwan: collaborator

Study Sites (1)

Taipei Medical University Hospital

Taipei, Taiwan, 110, Taiwan

Location

Related Publications (12)

• Brennecke J, Stark A, Russell RB, Cohen SM. Principles of microRNA-target recognition. PLoS Biol. 2005 Mar;3(3):e85. doi: 10.1371/journal.pbio.0030085.

  PMID: 15723116 BACKGROUND

• Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004 Jan 23;116(2):281-97. doi: 10.1016/s0092-8674(04)00045-5.

  PMID: 14744438 BACKGROUND

• Hilton C, Neville MJ, Karpe F. MicroRNAs in adipose tissue: their role in adipogenesis and obesity. Int J Obes (Lond). 2013 Mar;37(3):325-32. doi: 10.1038/ijo.2012.59. Epub 2012 Apr 24.

  PMID: 22531086 BACKGROUND

• Krotkiewski M, Bjorntorp P, Sjostrom L, Smith U. Impact of obesity on metabolism in men and women. Importance of regional adipose tissue distribution. J Clin Invest. 1983 Sep;72(3):1150-62. doi: 10.1172/JCI111040.

  PMID: 6350364 BACKGROUND

• Jiang L, Huang Q, Zhang S, Zhang Q, Chang J, Qiu X, Wang E. Hsa-miR-125a-3p and hsa-miR-125a-5p are downregulated in non-small cell lung cancer and have inverse effects on invasion and migration of lung cancer cells. BMC Cancer. 2010 Jun 22;10:318. doi: 10.1186/1471-2407-10-318.

  PMID: 20569443 RESULT

• Herrera BM, Lockstone HE, Taylor JM, Wills QF, Kaisaki PJ, Barrett A, Camps C, Fernandez C, Ragoussis J, Gauguier D, McCarthy MI, Lindgren CM. MicroRNA-125a is over-expressed in insulin target tissues in a spontaneous rat model of Type 2 Diabetes. BMC Med Genomics. 2009 Aug 18;2:54. doi: 10.1186/1755-8794-2-54.

  PMID: 19689793 RESULT

• John B, Enright AJ, Aravin A, Tuschl T, Sander C, Marks DS. Human MicroRNA targets. PLoS Biol. 2004 Nov;2(11):e363. doi: 10.1371/journal.pbio.0020363. Epub 2004 Oct 5.

  PMID: 15502875 RESULT

• Kanehisa M, Goto S. KEGG: kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 2000 Jan 1;28(1):27-30. doi: 10.1093/nar/28.1.27.

  PMID: 10592173 RESULT

• Gustafson B. Adipose tissue, inflammation and atherosclerosis. J Atheroscler Thromb. 2010 Apr 30;17(4):332-41. doi: 10.5551/jat.3939. Epub 2010 Feb 3.

  PMID: 20124732 RESULT

• O'Connell J, Lynch L, Cawood TJ, Kwasnik A, Nolan N, Geoghegan J, McCormick A, O'Farrelly C, O'Shea D. The relationship of omental and subcutaneous adipocyte size to metabolic disease in severe obesity. PLoS One. 2010 Apr 1;5(4):e9997. doi: 10.1371/journal.pone.0009997.

  PMID: 20376319 RESULT

• Wellen KE, Hotamisligil GS. Inflammation, stress, and diabetes. J Clin Invest. 2005 May;115(5):1111-9. doi: 10.1172/JCI25102.

  PMID: 15864338 RESULT

• Niswender KD, Morrison CD, Clegg DJ, Olson R, Baskin DG, Myers MG Jr, Seeley RJ, Schwartz MW. Insulin activation of phosphatidylinositol 3-kinase in the hypothalamic arcuate nucleus: a key mediator of insulin-induced anorexia. Diabetes. 2003 Feb;52(2):227-31. doi: 10.2337/diabetes.52.2.227.

  PMID: 12540590 RESULT

Biospecimen

Retention: NONE RETAINED

Paired samples of abdominal subcutaneous and intrabdominal omental adipose tissue were obtained from men and women who underwent laparoscopic bariatric surgery.

MeSH Terms

Conditions

Obesity, Morbid

Interventions

Sex

Condition Hierarchy (Ancestors)

Obesity; Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Reproductive Physiological Phenomena; Reproductive and Urinary Physiological Phenomena

Study Officials

• Wang Weu

  Comprehensive Weight Management Center Taipei Medical University Hospital

  STUDY DIRECTOR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: professor

Study Record Dates

• First Submitted: September 10, 2012
• First Posted: September 12, 2012
• Study Start: June 1, 2010
• Primary Completion: September 1, 2010
• Study Completion: September 1, 2010
• Last Updated: September 13, 2012

Record last verified: 2012-09

Locations

TW (1)