Effects of Beef Protein Consumption on Energy Intake

NCT01646749 | Completed

• 1 other identifier: NL41371
• Study Type: interventional
• Target: 30
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this study is to determine ad libitum daily energy intake, body weight changes and appetite profile in response to protein/carbohydrate and fat ratio over 12 consecutive days, and in relation to age, gender, body mass index (BMI) and fat mass and obesity-associated (FTO) gene alleles.

Conditions

Obesity; Severe Overweight

Keywords

Energy intake; Beef protein; Satiety; Protein leverage hypothesis

Outcome Measures

Primary Outcomes (3)

• Body weight change

  Body weight will be measured on day 1, 6 and 12. Subsequently, the change in body weight over time will be calculated.

  11 days

• Appetite profile

  Appetite profile will be measured by means of questionnaires: visual analogue scales (VAS). Area under the curve (AUC) will be calculated over 12 consecutive days.

  12 consecutive days

• Energy intake

  Total energy intake over 12 days will be determined for each subject by adding energy intake during meals to energy intake from snack consumption.

  12 consecutive days

Study Arms (3)

Protein intake of 5 energy percent (En%)

EXPERIMENTAL

Dietary Supplement: Differences in protein content (En%) of meals

Protein intake of 15 En%

EXPERIMENTAL

Dietary Supplement: Differences in protein content (En%) of meals

Protein intake of 30 En%

EXPERIMENTAL

Dietary Supplement: Differences in protein content (En%) of meals

Interventions

Differences in protein content (En%) of meals DIETARY_SUPPLEMENT

The three applied conditions will differ in the relative protein content of the meals, including 5 En%, 15 En% and 30 En% from protein. Beef protein will be used as main meat protein source in the 15 En% and 30 En% protein conditions. The resulting macronutrient compositions of the diets will be En% Protein/Carbohydrate/Fat; 5/60/35, 15/50/35, and 30/35/35. All meals (breakfast, lunch, dinner) within each condition will have the same macronutrient composition. All food items, and the energy density, weight and volume of the meals will be the same between conditions. All snack items will be very low in protein content.

Also known as: All food items will be commercial available.

Protein intake of 15 En%; Protein intake of 30 En%; Protein intake of 5 energy percent (En%)

Eligibility Criteria

• Age: 18 Years - 70 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Healthy
• Age 18-70 years
• BMI 18-35 kg/m2
• Non-smoking
• Weight stable

You may not qualify if:

• Smoking
• Use of medication
• More than moderate alcohol consumption
• Vegetarian

Sponsors & Collaborators

• Maastricht University Medical Center: lead
• National Cattlemen's Beef Association, a contractor to the Beef Checkoff: collaborator

Study Sites (1)

Maastricht University

Maastricht, 6200 MD, Netherlands

Location

Related Publications (7)

• Simpson SJ, Raubenheimer D. Obesity: the protein leverage hypothesis. Obes Rev. 2005 May;6(2):133-42. doi: 10.1111/j.1467-789X.2005.00178.x.

  PMID: 15836464 BACKGROUND

• Weigle DS, Breen PA, Matthys CC, Callahan HS, Meeuws KE, Burden VR, Purnell JQ. A high-protein diet induces sustained reductions in appetite, ad libitum caloric intake, and body weight despite compensatory changes in diurnal plasma leptin and ghrelin concentrations. Am J Clin Nutr. 2005 Jul;82(1):41-8. doi: 10.1093/ajcn.82.1.41.

  PMID: 16002798 BACKGROUND

• Griffioen-Roose S, Mars M, Siebelink E, Finlayson G, Tome D, de Graaf C. Protein status elicits compensatory changes in food intake and food preferences. Am J Clin Nutr. 2012 Jan;95(1):32-8. doi: 10.3945/ajcn.111.020503. Epub 2011 Dec 7.

  PMID: 22158729 BACKGROUND

• Gosby AK, Conigrave AD, Lau NS, Iglesias MA, Hall RM, Jebb SA, Brand-Miller J, Caterson ID, Raubenheimer D, Simpson SJ. Testing protein leverage in lean humans: a randomised controlled experimental study. PLoS One. 2011;6(10):e25929. doi: 10.1371/journal.pone.0025929. Epub 2011 Oct 12.

  PMID: 22022472 BACKGROUND

• Mikkelsen PB, Toubro S, Astrup A. Effect of fat-reduced diets on 24-h energy expenditure: comparisons between animal protein, vegetable protein, and carbohydrate. Am J Clin Nutr. 2000 Nov;72(5):1135-41. doi: 10.1093/ajcn/72.5.1135.

  PMID: 11063440 BACKGROUND

• Martens EA, Tan SY, Mattes RD, Westerterp-Plantenga MS. No protein intake compensation for insufficient indispensable amino acid intake with a low-protein diet for 12 days. Nutr Metab (Lond). 2014 Aug 20;11:38. doi: 10.1186/1743-7075-11-38. eCollection 2014.

  PMID: 25183991 DERIVED

• Martens EA, Tan SY, Dunlop MV, Mattes RD, Westerterp-Plantenga MS. Protein leverage effects of beef protein on energy intake in humans. Am J Clin Nutr. 2014 Jun;99(6):1397-406. doi: 10.3945/ajcn.113.078774. Epub 2014 Apr 23.

  PMID: 24760974 DERIVED

MeSH Terms

Conditions

Obesity; Overweight

Interventions

Meals

Condition Hierarchy (Ancestors)

Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Food; Diet, Food, and Nutrition; Physiological Phenomena; Food and Beverages

Study Officials

• Margriet S Westerterp-Plantenga, Prof. dr.

  Maastricht University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Purpose: TREATMENT
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: PhD student

Study Record Dates

• First Submitted: July 16, 2012
• First Posted: July 20, 2012
• Study Start: September 1, 2012
• Primary Completion: February 1, 2013
• Study Completion: February 1, 2013
• Last Updated: July 11, 2013

Record last verified: 2013-07

Locations

NL (1)