NCT01621867

Brief Summary

Cystic fibrosis is a genetic condition where epithelial cells, including from the respiratory tract, have an abnormal function of a surface protein, the cystic fibrosis transmembrane conductance regulator (CFTR) protein resulting from abnormal gene expression. The trial will assess the clinical efficacy, safety \& tolerability and gene expression following repeated nebulised doses of a gene product coding for a normal CFTR protein, with the primary outcome of the trial assessing lung function.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
130

participants targeted

Target at P75+ for phase_2

Timeline
Completed

Started May 2012

Geographic Reach
1 country

3 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

May 1, 2012

Completed
1 month until next milestone

First Submitted

Initial submission to the registry

June 14, 2012

Completed
4 days until next milestone

First Posted

Study publicly available on registry

June 18, 2012

Completed
1.9 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

May 1, 2014

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

May 1, 2014

Completed
Last Updated

October 22, 2015

Status Verified

November 1, 2012

Enrollment Period

2 years

First QC Date

June 14, 2012

Last Update Submit

October 21, 2015

Conditions

Keywords

Cystic fibrosisGene therapyCFTR geneGene expressionNon-viralMulti dose

Outcome Measures

Primary Outcomes (1)

  • Relative change in percent predicted FEV1 after 12 doses of gene product

    12-months

Secondary Outcomes (3)

  • EFFICACY

    12-months

  • SAFETY

    12-months

  • GENE EXPRESSION(subgroups only)

    12-months

Study Arms (2)

pGM169/GL67A (CFTR Gene/Lipid Vector)

ACTIVE COMPARATOR
Drug: pGM169/GL67A

Placebo

PLACEBO COMPARATOR
Drug: Placebo

Interventions

5ml Gene Product/Lipid Vector pGM169/GL67A nebulised; 2ml nasal application of pGM169/GL67A in addition to 5ml nebulised gene product (Nasal Subgroup)

pGM169/GL67A (CFTR Gene/Lipid Vector)

5ml Nebulised non-active placebo; 2ml nasal administration of non-active placebo (nasal subgroup)

Placebo

Eligibility Criteria

Age12 Years+
Sexall
Healthy VolunteersNo
Age GroupsChild (0-17), Adult (18-64), Older Adult (65+)

You may qualify if:

  • Cystic fibrosis confirmed by sweat testing or genetic analysis
  • Males and females aged 12 years and above
  • Forced expiratory volume in the 1st second (FEV1) between 50 \& 90% predicted inclusive (Stanojevic reference equations).
  • Clinical stability at screening defined by:
  • Not on any additional antibiotics (excluding routine, long-term treatments) for the previous 2 weeks
  • No increase in symptoms such as change in sputum production/colour, increased wheeze or breathlessness over the previous 2 weeks
  • No change in regular respiratory treatments over the previous 4 weeks
  • If any of these apply, entry into the study can be deferred
  • Prepared to take effective contraceptive precautions for the duration of their participation in the study and for 3 months thereafter (as stated in GTAC guidelines)
  • If taking regular rhDNase (pulmozyme) is willing, and considered able by independent medical carers, to withhold treatment for 24 hours before and 24 hours after the gene therapy dose (nebulised doses only)
  • Written informed consent obtained
  • Permission to inform their general practitioner of participation in study

You may not qualify if:

  • Infection with Burkholderia cepacia complex organisms, MRSA or M. abscessus
  • Significant nasal pathology including polyps, clinically-significant rhinosinusitis, or recurrent severe epistaxis (nose bleeds) (nasal cohort only)
  • Chloride secretory response on nasal PD of \> 5 mV (nasal cohort only; will only be known after first measurement)
  • Acute upper respiratory tract infection within the last 2 weeks (entry can be deferred)
  • Previous spontaneous pneumothorax without pleurodesis (bronchoscopic subgroup only)
  • Recurrent severe haemoptysis (bronchoscopic subgroup only)
  • Current smoker
  • Significant comorbidity including:
  • Moderate/severe CF liver disease (varices or significant, sustained elevation of transaminases: ALT/ AST\>100 IU/l)
  • Significant renal impairment (serum creatinine \> 150 mmol/l)
  • Significant coagulopathy (bronchoscopic group only)
  • Receiving 2nd line immunosuppressant drugs such as methotrexate, cyclosporine, intravenous immunoglobulin preparations
  • Pregnant or breastfeeding

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (3)

Western General Hospital

Edinburgh, EH4 2XU, United Kingdom

Location

Royal Hospital for Sick Children

Edinburgh, EH9 1LF, United Kingdom

Location

Royal Brompton Hospital

London, SW3 6NP, United Kingdom

Location

Related Publications (3)

  • Alton EW, Stern M, Farley R, Jaffe A, Chadwick SL, Phillips J, Davies J, Smith SN, Browning J, Davies MG, Hodson ME, Durham SR, Li D, Jeffery PK, Scallan M, Balfour R, Eastman SJ, Cheng SH, Smith AE, Meeker D, Geddes DM. Cationic lipid-mediated CFTR gene transfer to the lungs and nose of patients with cystic fibrosis: a double-blind placebo-controlled trial. Lancet. 1999 Mar 20;353(9157):947-54. doi: 10.1016/s0140-6736(98)06532-5.

  • Miah KM, Hyde SC, Gill DR. Emerging gene therapies for cystic fibrosis. Expert Rev Respir Med. 2019 Aug;13(8):709-725. doi: 10.1080/17476348.2019.1634547. Epub 2019 Jun 27.

  • Alton EWFW, Armstrong DK, Ashby D, Bayfield KJ, Bilton D, Bloomfield EV, Boyd AC, Brand J, Buchan R, Calcedo R, Carvelli P, Chan M, Cheng SH, Collie DDS, Cunningham S, Davidson HE, Davies G, Davies JC, Davies LA, Dewar MH, Doherty A, Donovan J, Dwyer NS, Elgmati HI, Featherstone RF, Gavino J, Gea-Sorli S, Geddes DM, Gibson JSR, Gill DR, Greening AP, Griesenbach U, Hansell DM, Harman K, Higgins TE, Hodges SL, Hyde SC, Hyndman L, Innes JA, Jacob J, Jones N, Keogh BF, Limberis MP, Lloyd-Evans P, Maclean AW, Manvell MC, McCormick D, McGovern M, McLachlan G, Meng C, Montero MA, Milligan H, Moyce LJ, Murray GD, Nicholson AG, Osadolor T, Parra-Leiton J, Porteous DJ, Pringle IA, Punch EK, Pytel KM, Quittner AL, Rivellini G, Saunders CJ, Scheule RK, Sheard S, Simmonds NJ, Smith K, Smith SN, Soussi N, Soussi S, Spearing EJ, Stevenson BJ, Sumner-Jones SG, Turkkila M, Ureta RP, Waller MD, Wasowicz MY, Wilson JM, Wolstenholme-Hogg P; UK Cystic Fibrosis Gene Therapy Consortium. Repeated nebulisation of non-viral CFTR gene therapy in patients with cystic fibrosis: a randomised, double-blind, placebo-controlled, phase 2b trial. Lancet Respir Med. 2015 Sep;3(9):684-691. doi: 10.1016/S2213-2600(15)00245-3. Epub 2015 Jul 3.

Related Links

MeSH Terms

Conditions

Cystic Fibrosis

Condition Hierarchy (Ancestors)

Pancreatic DiseasesDigestive System DiseasesLung DiseasesRespiratory Tract DiseasesGenetic Diseases, InbornCongenital, Hereditary, and Neonatal Diseases and AbnormalitiesInfant, Newborn, Diseases

Study Officials

  • Eric Alton, MD, FMedSci

    Imperial College London

    STUDY DIRECTOR
  • Jane Davies, MD

    Imperial College London

    PRINCIPAL INVESTIGATOR
  • Uta Griesenbach, PhD

    Imperial College London

    PRINCIPAL INVESTIGATOR
  • Steve Hyde, MA, DPhil

    University of Oxford

    PRINCIPAL INVESTIGATOR
  • Deborah Gill, PhD

    University of Oxford

    PRINCIPAL INVESTIGATOR
  • Chris Boyd, PhD

    Edinburgh University

    PRINCIPAL INVESTIGATOR
  • David Porteous, FMedSci

    Edinburgh University

    PRINCIPAL INVESTIGATOR
  • Alastair Innes, PhD

    Edinburgh University

    PRINCIPAL INVESTIGATOR
  • Steve Cunningham, PhD

    Edinburgh University

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
phase 2
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, INVESTIGATOR
Purpose
TREATMENT
Intervention Model
SINGLE GROUP
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

June 14, 2012

First Posted

June 18, 2012

Study Start

May 1, 2012

Primary Completion

May 1, 2014

Study Completion

May 1, 2014

Last Updated

October 22, 2015

Record last verified: 2012-11

Locations