NCT01598870

Brief Summary

The study is aimed to revise the current criteria for the diagnosis of spontaneous bacterial peritonitis and the decisional thresholds to start treatment. The accuracy of current diagnostic tests will be compared with that of new tests, which could potentially become more accurate reference standards. This could lead to the definition of a more accurate and effective diagnostic algorithm.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
100

participants targeted

Target at P50-P75 for all trials

Timeline
Completed

Started May 2012

Typical duration for all trials

Geographic Reach
1 country

1 active site

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Start

First participant enrolled

May 1, 2012

Completed
10 days until next milestone

First Submitted

Initial submission to the registry

May 11, 2012

Completed
4 days until next milestone

First Posted

Study publicly available on registry

May 15, 2012

Completed
3.1 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

July 1, 2015

Completed
3 months until next milestone

Study Completion

Last participant's last visit for all outcomes

October 1, 2015

Completed
Last Updated

January 14, 2015

Status Verified

January 1, 2015

Enrollment Period

3.2 years

First QC Date

May 11, 2012

Last Update Submit

January 13, 2015

Conditions

Keywords

Spontaneous bacterial peritonitis.

Outcome Measures

Primary Outcomes (1)

  • Comparing the accuracy of current reference standard for the diagnosis of spontaneous bacterial peritonitis, i.e. neutrophil count in ascitic fluid of >250/mm3 as determined by microscopy, with alternative diagnostic tests.

    Reference standard is neutrophil count in ascitic fluid of \>250/mm3 as determined by microscopy. Alternative diagnostic tests are: Leukocyte count by automated counters; reagent strips for leukocyte esterase and for lactoferrin; ascitic fluid pH; leukocyte cytometry; bacterial DNA detection and quantification. Discrepancies between reference standard and alternative tests will be assessed by one month clinical follow up.

    One month

Study Arms (1)

Patients with cirrhosis and ascites

Patients requiring diagnostic and/or therapeutic paracentesis.

Other: Diagnostic tests

Interventions

Leukocyte count by automated counters; reagent strips for leukocyte esterase and for lactoferrin; ascitic fluid pH; leukocyte cytometry; bacterial DNA detection and quantification.

Patients with cirrhosis and ascites

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Patients with cirrhosis and ascites requiring paracentesis.

You may qualify if:

  • Patients with cirrhosis and ascites with clinical indication to carry out a diagnostic and/or therapeutic paracentesis.

You may not qualify if:

  • Etiology of ascites other than cirrhosis

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Department of Medicine, A. Manzoni Hospital

Lecco, 23900, Italy

RECRUITING

Biospecimen

Retention: SAMPLES WITH DNA

Blood and ascitic fluid samples stored at -80°C

Study Officials

  • Agostino Colli, MD

    AO Provincia di Lecco, Department of Medicine, A. Manzoni Hospital, Lecco

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
MD, Head of Internal Medicine Department AO Provincia di Lecco

Study Record Dates

First Submitted

May 11, 2012

First Posted

May 15, 2012

Study Start

May 1, 2012

Primary Completion

July 1, 2015

Study Completion

October 1, 2015

Last Updated

January 14, 2015

Record last verified: 2015-01

Locations