Invasiveness and Chemoresistance of Cancer Stem Cells in Colon Cancer

NCT01577511 | Completed

• 2 other identifiers: LOCAL/2011/JFB-012011-A01141-40
• Study Type: observational
• Target: 60
• Locations: 1 country
• Sites: 1

Brief Summary

The main objective of this study is to identify and characterize subpopulations of cells with invasive capacity in colorectal cancer from primary tumor, blood and metastatic samples.

Conditions

Colorectal Neoplasms

Keywords

invasive potential; molecular characterisation of circulating cells

Outcome Measures

Primary Outcomes (1)

• Ability to maintain the cells isolated from colorectal tumors in culture or 3D collagen matrices and then infect these cells to make them express reporter genes: yes/no.

  Baseline (Day 0)

Secondary Outcomes (21)

• Serology HIV

  baseline; day 0

• Serology Hepatitis B

  baseline; day 0

• Serology Hepatitis C

  baseline; day 0

• Location of primary tumor

  base line; day 0

• Age at diagnosis

  baseline, day 0

Study Arms (1)

30 Patients

Patients with operable, stage III or IV, adenocarcino-type colorectal cancer treated at the Nîmes University Hospital. Intervention: Samples and follow up

Biological: Samples and follow up

Interventions

Samples and follow up BIOLOGICAL

Samples: Peroperative blood sample plus primary and metastatic tumor biopsies. Follow-up: disease outcomes assessed at 24 months

30 Patients

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Patients with operable, stage III or IV, adenocarcino-type colorectal cancer treated at the Nîmes University Hospital.

You may qualify if:

• The patient must have given his/her informed and signed consent
• The patient must be insured or beneficiary of a health insurance plan
• Patient with adenocarcinoma-type colorectal cancer:
• stage III at diagnosis, surgical resection of the primary tumor proposed.
• stage IV at diagnosis, surgical resection of the primary tumor and possibly of metastases proposed.
• Stage IV who have already undergone surgical excision of the primary tumor, and for whom metastasectomy is now proposed.

You may not qualify if:

• The patient is participating in another study
• The patient is under judicial protection, under tutorship or curatorship
• The patient refuses to sign the consent
• It is impossible to correctly inform the patient
• Patients for whom surgical resection of the primary tumor is not considered as an option
• PStage IV at diagnosis, but metastasectomy is not considered as an option
• Patients with positive HIV, Hepatitis B or Hepatitis C serology

Sponsors & Collaborators

• Centre Hospitalier Universitaire de Nīmes: lead

Study Sites (1)

CHU de Nîmes - Hôpital Universitaire Carémeau

Nîmes, Gard, 30029, France

Location

Related Publications (1)

• Grillet F, Bayet E, Villeronce O, Zappia L, Lagerqvist EL, Lunke S, Charafe-Jauffret E, Pham K, Molck C, Rolland N, Bourgaux JF, Prudhomme M, Philippe C, Bravo S, Boyer JC, Canterel-Thouennon L, Taylor GR, Hsu A, Pascussi JM, Hollande F, Pannequin J. Circulating tumour cells from patients with colorectal cancer have cancer stem cell hallmarks in ex vivo culture. Gut. 2017 Oct;66(10):1802-1810. doi: 10.1136/gutjnl-2016-311447. Epub 2016 Jul 25.

  PMID: 27456153 RESULT

Biospecimen

Retention: SAMPLES WITHOUT DNA

Peroperative blood sample plus primary and metastatic tumor biopsies.

MeSH Terms

Conditions

Colorectal Neoplasms

Condition Hierarchy (Ancestors)

Intestinal Neoplasms; Gastrointestinal Neoplasms; Digestive System Neoplasms; Neoplasms by Site; Neoplasms; Digestive System Diseases; Gastrointestinal Diseases; Colonic Diseases; Intestinal Diseases; Rectal Diseases

Study Officials

• Jean-François Bourgaux, MD

  Centre Hospitalier Universitaire de Nîmes

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: April 12, 2012
• First Posted: April 16, 2012
• Study Start: June 12, 2012
• Primary Completion: December 31, 2016
• Study Completion: December 31, 2016
• Last Updated: November 19, 2025

Record last verified: 2020-01

Locations

FR (1)