NCT01570036

Brief Summary

The study will be a multi-center, prospective, randomized, single-blinded, placebo-controlled Phase II trial of Herceptin + NeuVax(TM) vaccine (E75 peptide/granulocyte macrophage-colony stimulating factor) (GM-CSF) versus Herceptin + GM-CSF alone. The target study population is node-positive (NP) (or node-negative \[NN\] if negative for both ER and PR) breast cancer patients with HER2 1+ and 2+ expressing tumors who are disease-free after standard of care therapy. Disease-free subjects after standard of care multi-modality therapy will be screened and HLA-typed. E75 is a CD8-eliciting peptide vaccine that was restricted to HLA-A2+ or HLA-A3+ patients (approximately two-thirds of the US population), and has been extended to HLA-A24+ and HLA-A26+ as well.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
275

participants targeted

Target at P75+ for phase_2 breast-cancer

Timeline
Completed

Started May 2013

Typical duration for phase_2 breast-cancer

Geographic Reach
1 country

29 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

March 25, 2012

Completed
10 days until next milestone

First Posted

Study publicly available on registry

April 4, 2012

Completed
1.1 years until next milestone

Study Start

First participant enrolled

May 21, 2013

Completed
5.4 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

September 28, 2018

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

September 28, 2018

Completed
2.2 years until next milestone

Results Posted

Study results publicly available

December 2, 2020

Completed
Last Updated

December 2, 2020

Status Verified

November 1, 2020

Enrollment Period

5.4 years

First QC Date

March 25, 2012

Results QC Date

June 17, 2019

Last Update Submit

November 9, 2020

Conditions

Keywords

stage I breast cancerstage II breast cancerstage IIIA breast cancerstage IIIB breast cancerstage IIIC breast cancermale breast cancer

Outcome Measures

Primary Outcomes (2)

  • Disease-free Survival (DFS)

    Disease-free survival (DFS) for all patients regardless of randomization will be determined by patients' own physicians at the individual study sites during routine follow-up screening. This will occur every three months for the first 24 months after completion of primary therapies and every six months thereafter with clinical exam, and laboratory and radiographic surveillance. The primary objective of the study is disease-free survival (DFS) at 24 months.

    Disease-free survival at 24 months

  • Disease-free Survival (DFS)

    Disease-free survival (DFS) for all patients regardless of randomization will be determined by patients' own physicians at the individual study sites during routine follow-up screening. This will occur at months 30 and 36 after completion of primary therapies with clinical exam, and laboratory and radiographic surveillance. The secondary objective of the study is disease-free survival (DFS) at 36 months.

    Disease-free survival up to 36 months

Secondary Outcomes (2)

  • Percent Ejection Fraction - A Measure of Cardiac Toxicity

    24 months

  • Local and Systemic Toxicities

    Duration of vaccine or inoculation series and booster series, an average of 30 months.

Study Arms (2)

Herceptin + NeuVax vaccine

EXPERIMENTAL

Patients randomized to this arm will receive Herceptin every 3 weeks as monotherapy for 1 year; the first Herceptin infusion will be given no sooner than 3 weeks and no later than 12 weeks after completion of standard of care chemotherapy/radiotherapy. Herceptin will be dosed at the recommended initial loading dose of 8 mg/kg and at recommended maintenance doses of 6 mg/kg q3wk. Patients will receive vaccinations of NeuVax vaccine administered intradermally every 3 weeks for 6 total vaccinations, 30-120 minutes after completion of Herceptin infusion. The NeuVax vaccine series will begin immediately after completion of the third Herceptin infusion, but may be delayed to the fourth or fifth Herceptin infusion with prior approval from the PI. Patients will be blinded regarding assigned arm. After completion of primary vaccine series, patients will receive 4 NeuVax vaccine booster inoculations to be administered every 6 months x 4 for total treatment duration of 30 months.

Drug: HerceptinDrug: NeuVax vaccineDrug: GM-CSF

Herceptin + GM-CSF only

ACTIVE COMPARATOR

Patients randomized to this arm will receive Herceptin every 3 weeks as monotherapy for 1 year. The first Herceptin infusion will be given no sooner than 3 weeks and no later than 12 weeks after completion of standard of care chemotherapy/radiotherapy. Herceptin will be dosed at the recommended initial loading dose of 8 mg/kg and at recommended maintenance doses of 6 mg/kg q3wk. Patients will receive inoculations of GM-CSF only (250mcg) administered intradermally every 3 weeks for 6 total inoculations, 30-120 minutes after completion of Herceptin infusion. The GM-CSF only inoculation series will begin immediately after completion of the third Herceptin infusion. Patients will be blinded as to whether they are receiving NeuVax vaccine or GM-CSF only. After completion of six-inoculation primary vaccine series, patients will then receive a total of four GM-CSF only booster inoculations to be administered at 12, 18, 24, and 30 months from the date of the first Herceptin infusion.

Drug: HerceptinDrug: GM-CSF

Interventions

Herceptin will be administered to patients every three weeks as monotherapy for one year, to be given upon completion of standard of care chemotherapy/radiotherapy. The first Herceptin infusion will be given no sooner than three weeks and no later than 12 weeks after completion of chemotherapy/radiotherapy. Herceptin will be dosed at the recommended initial loading dose of 8 mg/kg and at recommended maintenance doses of 6 mg/kg q3wk.

Also known as: Trastuzumab
Herceptin + GM-CSF onlyHerceptin + NeuVax vaccine

At the time of vaccine administration, a frozen solution of E75 acetate (1.5mg/ml) is thawed and 1000mcg E75 peptide mixed thoroughly with 250mcg GM-CSF. This constitutes the NeuVax vaccine. For patients randomized to the Herceptin + NeuVax vaccine arm, they will commence Herceptin monotherapy and then will begin the NeuVax vaccine series immediately after completion of the third Herceptin infusion. The vaccine series consists of NeuVax vaccine administered intradermally every three weeks for six total vaccinations, 30-120 minutes after completion of Herceptin infusion.

Also known as: E75 peptide (KIFGSLAFL, HER2/neu, 369-377), GM-CSF (sargramostim)
Herceptin + NeuVax vaccine
GM-CSFDRUG

For patients randomized to the Herceptin + GM-CSF only arm, they will commence Herceptin monotherapy and then will begin the GM-CSF inoculation series immediately after completion of the third Herceptin infusion. The GM-CSF inoculation series consists of 250mcg GM-CSF administered intradermally every three weeks for six total vaccinations, 30-120 minutes after completion of Herceptin infusion.

Also known as: Sargramostim
Herceptin + GM-CSF onlyHerceptin + NeuVax vaccine

Eligibility Criteria

Age18 Years+
Sexfemale
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may not qualify if:

  • Patients will be excluded from the study based on the following criteria:
  • Node-negative breast cancer (AJCC N0 or N0(i+)) unless negative for both estrogen (ER) and progesterone (PR) receptors and has received chemotherapy as standard of care
  • Clinical or radiographic evidence of distant or residual breast cancer
  • HER2 negative (IHC 0) or HER2 3+ or FISHDual-ISH amplified (FISH \>2.0); Dual-ISH \>2.0
  • HLA-A2, A3, A24, A26 negative
  • History of prior Herceptin therapy
  • NYHA stage 3 or 4 cardiac disease
  • LVEF \<50%, or less than the normal limits of the institution's specific testing (MUGA or Echo)
  • Immune deficiency disease or HIV, HBV, HCV
  • Receiving immunosuppressive therapy including chemotherapy, chronic steroids, methotrexate, or other known immunosuppressive agents
  • ECOG ≥2
  • Tbili \>1.8, creatinine\>2, hemoglobin\<10, platelets\<50,000, WBC\<2,000
  • Pregnancy (assessed by urine HCG)
  • Breast feeding
  • Any active autoimmune disease requiring treatment, with the exception of vitiligo
  • +2 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (29)

Samuel Oschin Comprehensive Cancer Institute - Cedars Sinai Medical Center

Beverly Hills, California, 90211, United States

Location

Sarcoma Oncology Research Center, LLC

Santa Monica, California, 90403, United States

Location

St. Joseph Heritage Healthcare

Santa Rosa, California, 95403, United States

Location

Sibley Memorial Hospital

Washington D.C., District of Columbia, 20016, United States

Location

Katzen Cancer Research Center, George Washington University

Washington D.C., District of Columbia, 20037, United States

Location

University of Miami

Deerfield Beach, Florida, 33442, United States

Location

University of Miami

Kendall, Florida, 33176, United States

Location

University of Miami

Miami, Florida, 33136, United States

Location

Florida Cancer Research Institute

Plantation, Florida, 33324, United States

Location

University of Miami

Plantation, Florida, 33324, United States

Location

H. Lee Moffitt Cancer Center & Research Institute, Inc

Tampa, Florida, 33612, United States

Location

University of Hawaii Cancer Center

Honolulu, Hawaii, 96813, United States

Location

Franciscan Health Indianapolis

Indianapolis, Indiana, 46237, United States

Location

Memorial Hospital of South Bend

South Bend, Indiana, 46601, United States

Location

Cancer Center of Kansas

Wichita, Kansas, 67212, United States

Location

Medstar Health - Union Memorial Hospital

Baltimore, Maryland, 21218-2895, United States

Location

Medstar Health - Weinberg Cancer Institute at Franklin Square

Baltimore, Maryland, 21237, United States

Location

MedStar Health - Good Samaritan Hospital

Baltimore, Maryland, 21239, United States

Location

The Valley Hospital

Paramus, New Jersey, 07652, United States

Location

Tisch Cancer Institute/Icahn School of Medicine at Mount Sinai

New York, New York, 10029, United States

Location

North Shore Hematology Oncology Associates

The Bronx, New York, 10469, United States

Location

Legacy Health, Legacy Good Samaritan Medical Center

Portland, Oregon, 97210, United States

Location

Thomas Jefferson University - Kimmel Cancer Center

Philadelphia, Pennsylvania, 19107, United States

Location

University of Texas M.D. Anderson Cancer Center

Houston, Texas, 77030, United States

Location

Texas Oncology (Cancer Care Centers of South Texas)

San Antonio, Texas, 78217, United States

Location

Virginia Cancer Specialists

Fairfax, Virginia, 22031, United States

Location

Providence Regional Medical Center

Everett, Washington, 98201, United States

Location

Swedish Cancer Institute

Seattle, Washington, 98104, United States

Location

Columbia St. Mary's

Milwaukee, Wisconsin, 53211, United States

Location

Related Publications (1)

  • Clifton GT, Hale D, Vreeland TJ, Hickerson AT, Litton JK, Alatrash G, Murthy RK, Qiao N, Philips AV, Lukas JJ, Holmes JP, Peoples GE, Mittendorf EA. Results of a Randomized Phase IIb Trial of Nelipepimut-S + Trastuzumab versus Trastuzumab to Prevent Recurrences in Patients with High-Risk HER2 Low-Expressing Breast Cancer. Clin Cancer Res. 2020 Jun 1;26(11):2515-2523. doi: 10.1158/1078-0432.CCR-19-2741. Epub 2020 Feb 18.

MeSH Terms

Conditions

Breast NeoplasmsBreast Neoplasms, Male

Interventions

TrastuzumabHER2 peptide (369-377)Receptor Protein-Tyrosine KinasesGranulocyte-Macrophage Colony-Stimulating Factorsargramostim

Condition Hierarchy (Ancestors)

Neoplasms by SiteNeoplasmsBreast DiseasesSkin DiseasesSkin and Connective Tissue Diseases

Intervention Hierarchy (Ancestors)

Antibodies, Monoclonal, HumanizedAntibodies, MonoclonalAntibodiesImmunoglobulinsImmunoproteinsBlood ProteinsProteinsAmino Acids, Peptides, and ProteinsSerum GlobulinsGlobulinsProtein-Tyrosine KinasesProtein KinasesPhosphotransferases (Alcohol Group Acceptor)PhosphotransferasesTransferasesEnzymesEnzymes and CoenzymesIntracellular Signaling Peptides and ProteinsReceptors, Cell SurfaceMembrane ProteinsColony-Stimulating FactorsGlycoproteinsGlycoconjugatesCarbohydratesHematopoietic Cell Growth FactorsCytokinesIntercellular Signaling Peptides and ProteinsPeptidesBiological Factors

Results Point of Contact

Title
Program Director, Karen Arrington, RN, BSN
Organization
Cancer Insight

Study Officials

  • COL (ret.) George E. Peoples, MD, FACS

    Cancer Insight, LLC

    PRINCIPAL INVESTIGATOR
  • COL (ret.) George E. Peoples, MD, FACS

    Cancer Insight, LLC

    STUDY DIRECTOR

Publication Agreements

PI is Sponsor Employee
No
Restriction Type
OTHER
Restrictive Agreement
Yes

Study Design

Study Type
interventional
Phase
phase 2
Allocation
RANDOMIZED
Masking
SINGLE
Who Masked
PARTICIPANT
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR INVESTIGATOR
PI Title
President and CEO, Cancer Insight, LLC

Study Record Dates

First Submitted

March 25, 2012

First Posted

April 4, 2012

Study Start

May 21, 2013

Primary Completion

September 28, 2018

Study Completion

September 28, 2018

Last Updated

December 2, 2020

Results First Posted

December 2, 2020

Record last verified: 2020-11

Locations