NCT01550133

Brief Summary

This study has two main aims: 1) To determine if ingestion of solid or beverage food forms will change appetite hormone responses, and 2) To determine whether "learning" (defined as 2 week daily consumption) about the metabolic consequence of ingesting solid or beverage foods forms varying in energy alters appetite hormone responses. Beverage consumption has been implicated in the problem of obesity. However, the exact relationship between beverages, lower appetitive response and lower compensatory dietary responses remains unclear. This study aims to address this gap in the research. For aim 1, the null hypothesis is that the energy in beverage and solid forms will not affect appetite hormonal responses differently. The alternative hypothesis is that exposure to the energy-yielding beverage will elicit a lower appetitive hormone response compared to oral exposure to the solid food form. For aim 2, the null hypothesis is that learning will not change appetite hormone responses. The alternative hypothesis is that learning will decrease appetite hormone responses in the non-energy-yielding beverage more than in the energy-yielding beverage.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
60

participants targeted

Target at P25-P50 for not_applicable

Timeline
Completed

Started Sep 2012

Longer than P75 for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

March 7, 2012

Completed
2 days until next milestone

First Posted

Study publicly available on registry

March 9, 2012

Completed
6 months until next milestone

Study Start

First participant enrolled

September 1, 2012

Completed
3.7 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 30, 2016

Completed
8 months until next milestone

Study Completion

Last participant's last visit for all outcomes

December 30, 2016

Completed
Last Updated

December 12, 2023

Status Verified

December 1, 2023

Enrollment Period

3.7 years

First QC Date

March 7, 2012

Last Update Submit

December 11, 2023

Conditions

Keywords

PeptidesHormonesAppetiteHumansObeseBeverageSolid foodcephalic phase

Outcome Measures

Primary Outcomes (1)

  • Appetite Hormone Response

    Determine the appetite hormone responses to oral exposure of nutritive (energy-yielding) or non-nutritive (non-energy-yielding) sweetener in beverage or solid form. Then, determine whether the appetite hormone responses change after a 2-week learning period. Appetite hormones to be measured: Cholecystokinin, Glucagon-Like-Peptide-1, Ghrelin, Insulin, Pancreatic Polypeptide

    week 1, week 2, week 3, week 4, week 7, week 8, week 9, week 10 (once a week over 136 minutes: 0, 14, 16, 18, 20, 22, 24, 26, 28, 30, 76, 106, 136 minutes)

Secondary Outcomes (3)

  • Change in Appetite

    week 1, week 2, week 3, week 4, week 7, week 8, week 9, week 10 (once a week, over 180 minutes: 0, 14, 30, 76, 106, 136, 180 minutes)

  • Plasma Glucose

    week 1, week 2, week 3, week 4, week 7, week 8, week 9, week 10 (once a week, over 136 minutes: 0, 14, 16, 18, 20, 22, 24, 26, 28, 30, 76, 106, 136 minutes)

  • Amount of meal ingested

    week 1, week 2, week 3, week 4, week 7, week 8, week 9, week 10 (once a week, at 137 minutes to 180 minutes)

Study Arms (8)

Not Learned: Non-nutritive bev to Nutritive bev

EXPERIMENTAL

15 participants will be randomly assigned to eat 250 grams of non-nutritive solid consecutively for 2 weeks. After this conditioning, the cephalic phase response of half of these participants will be tested against a nutritive solid and act as a control for the learned effects.

Behavioral: Learned: Non-nutritive beverage to Non-nutritive beverage

Not Learned: Nutritive solid to Non-Nutritive solid

EXPERIMENTAL

15 participants will be randomly assigned to eat 250 grams of nutritive solid consecutively for 2 weeks. After this conditioning, the cephalic phase response of half of these participants will be tested against a non-nutritive solid and act as a control for the learned effects.

Behavioral: Learned: Nutritive solid to Nutritive solid

Not Learned: Nutritive beverage to Non-Nutritive beverage

EXPERIMENTAL

15 participants will be randomly assigned to drink 400 grams of nutritive beverage consecutively for 2 weeks. After this conditioning, the cephalic phase response of half of these participants will be tested against a non-nutritive beverage and act as a control for the learned effects.

Behavioral: Learned: Nutritive beverage to Nutritive beverage

Learned: Non-Nutritive solid to Non-Nutritive solid

EXPERIMENTAL

15 participants will be randomly assigned to eat 250 grams of non-nutritive solid consecutively for 2 weeks. After this conditioning, the cephalic phase response of half of these participants will be tested against a non-nutritive solid to determine if cephalic phase response changes with learning.

Behavioral: Not Learned: Non-nutritive solid to Nutritive solid

Learned: Non-Nutritive beverage to Non-Nutritive beverage

EXPERIMENTAL

15 participants will be randomly assigned to drink 400 grams of non-nutritive beverage consecutively for 2 weeks. After this conditioning, the cephalic phase response of half of these participants will be tested against a non-nutritive beverage to determine if cephalic phase response changes with learning.

Behavioral: Not Learned: Nutritive beverage to Non-nutritive beverage

Learned: Nutritive solid to Nutritive solid

EXPERIMENTAL

15 participants will be randomly assigned to eat 250 grams of nutritive solid consecutively for 2 weeks. After this conditioning, the cephalic phase response of half of these participants will be tested against a nutritive solid to determine if cephalic phase response changes with learning.

Behavioral: Not Learned: Non-Nutritive solid to Non-nutritive solid

Learned: Nutritive beverage to Nutritive beverage

EXPERIMENTAL

15 participants will be randomly assigned to drink 400 grams of nutritive beverage consecutively for 2 weeks. After this conditioning, the cephalic phase response of half of these participants will be tested against a nutritive beverage to determine if cephalic phase response changes with learning.

Behavioral: Not Learned: Non-nutritive beverage to Nutritive beverage

Not Learned: Non-Nutritive solid to Non-Nutritive solid

EXPERIMENTAL

15 participants will be randomly assigned to eat 250 grams of non-nutritive solid consecutively for 2 weeks. After this conditioning, the cephalic phase response of half of these participants will be tested against a non-nutritive solid to determine if cephalic phase response changes with learning.

Behavioral: Not Learned: Non-Nutritive solid to Non-Nutritive Solid

Interventions

For the learning phase of the experiment, random allocation will assign about 15 participants to consume a beverage product made with non-nutritive sweetener everyday for two weeks.Half of these participants will then be tested against a non-nutritive beverage.

Also known as: NNB to NNB
Not Learned: Non-nutritive bev to Nutritive bev

For the learning phase of the experiment, random allocation will assign about 15 participants to consume a solid food product made with nutritive sweetener everyday for two weeks. Half of these participants will then be tested against a nutritive solid.

Also known as: NS to NS
Not Learned: Nutritive solid to Non-Nutritive solid

For the learning phase of the experiment, random allocation will assign about 15 participants to consume a beverage product made with nutritive sweetener everyday for two weeks. Half of these participants will then be tested against a nutritive beverage.

Also known as: NB to NB
Not Learned: Nutritive beverage to Non-Nutritive beverage

For the learning phase of the experiment, random allocation will assign about 15 participants to consume a solid food product made with non-nutritive sweetener everyday for two weeks. Half of these participants will then be tested against a nutritive solid.

Also known as: NNS to NS
Learned: Non-Nutritive solid to Non-Nutritive solid

For the learning phase of the experiment, random allocation will assign about 15 participants to consume a beverage food product made with nutritive sweetener everyday for two weeks. Half of these participants will then be tested against a non-nutritive beverage.

Also known as: NB to NNB
Learned: Non-Nutritive beverage to Non-Nutritive beverage

For the learning phase of the experiment, random allocation will assign about 15 participants to consume a solid food product made with nutritive sweetener everyday for two weeks. Half of these participants will then be tested against a non-nutritive solid.

Also known as: NS to NNS
Learned: Nutritive solid to Nutritive solid

For the learning phase of the experiment, random allocation will assign about 15 participants to consume a beverage food product made with non-nutritive sweetener everyday for two weeks. Half of these participants will then be tested against a nutritive beverage.

Also known as: NNB to NB
Learned: Nutritive beverage to Nutritive beverage

Eligibility Criteria

Age18 Years - 50 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Body fat percentage \> 25% for men and \> 32% for women. BMI of 24 kg/m2- 37kg/m2
  • No purposeful use of foods or beverages that are sweetened with non-nutritive sweetener \> 3 times a week
  • No purposeful addition of non-nutritive sweetener to foods and beverages \>3 times a week
  • No Phenylketonuria (PKU)
  • Rate palatability of test load and food sample as greater than 5 on a 9 point category scale.
  • Be willing to eat at least 100 grams of carbohydrates for three days prior to each test day
  • Self-reported consumer of breakfast and lunch
  • Be willing to eat macaroni and cheese during breakfast-lunch hours
  • Not taking medications known to influence appetite
  • Non-smoker \>1 year or more
  • Habitual activity pattern over the last 3 months and no plan to change this over the course of the study
  • Not on any extreme diet (e.g. Atkins Diet)
  • Weight stable (≤ 5 kg change over the last 3 months)
  • Not a restrained eater (have a dietary restraint score of \<9 on the Three Factor Eating Questionnaire-Restraint section)
  • Not a disordered eater (Have a score of \<20 in the Eating Attitudes Test-26)
  • +2 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Purdue University

West Lafayette, Indiana, 47907, United States

Location

MeSH Terms

Conditions

ObesityOverweight

Condition Hierarchy (Ancestors)

OvernutritionNutrition DisordersNutritional and Metabolic DiseasesBody WeightSigns and SymptomsPathological Conditions, Signs and Symptoms

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
SINGLE
Who Masked
PARTICIPANT
Purpose
BASIC SCIENCE
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Distinguished Professor

Study Record Dates

First Submitted

March 7, 2012

First Posted

March 9, 2012

Study Start

September 1, 2012

Primary Completion

April 30, 2016

Study Completion

December 30, 2016

Last Updated

December 12, 2023

Record last verified: 2023-12

Locations