Allogeneic Stem Cell Transplantation for Advanced Neuroblastoma Using MHC Mismatched Related Donors

NCT01462396 | Completed Phase 1 DMC

• 1 other identifier: STALLO
• Study Type: interventional
• Target: 4
• Locations: 1 country
• Sites: 1

Brief Summary

Allogeneic stem cell transplantation has been explored for patients with high risk neuroblastoma. Results have been mixed, with only small series and case reports. Recent reports, however, especially with haploidentical transplantation have been more encouraging. Eradication of neuroblastoma may be mediated by both components of the innate immune system (natural killer cells) and through the adaptive immune system via T-cell cytotoxicity and the development of a humoral response to tumor specific antigens and minor histocompatibility antigens. To overcome restrictions created by unavailability of Human leukocyte antigen (HLA) matched donors, stem cell grafts from haploidentical related donors have been explored. Historically, the use of full haplotype mismatched family member donors has been limited by the development of severe graft-versus-host disease and the high rate of graft failure. Graft failure can now be overcome by increasing immunosuppression and increasing the number of transplanted stem cells. The most effective means of graft versus host disease (GVHD) prophylaxis is T cell depletion of the donor marrow. A 3-4 log depletion will reduce the risk of developing significant GVHD to less than 10%. Methods to mobilize stem cells from the bone marrow into the peripheral blood and collect these stem cells by apheresis now increase the availability of stem cells by a magnitude. Selection devices have been developed that will prepare extremely pure populations of these CD34 cells with upwards of 5 logs depletion of contaminating T cells. The CliniMACS CD34 Reagent System is a medical device designed to select CD34+ hematopoietic cells from heterogeneous hematologic cell populations. The investigators intend to provide mismatched related hematopoietic stem cell transplantation to up to 10 patients with relapsed refractory neuroblastoma. Harnessing the potential for innate and adaptive immune responses through allogeneic Hematopoietic stem cell transplantation (HSCT) may provide cure for some patients with this tumor.

Conditions

Neuroblastoma

Keywords

Neuroblastoma; Relapsed; Refractory

Outcome Measures

Primary Outcomes (1)

• The immediate safety of a fludarabine based reduced intensity conditioning regimen and CD34+ stem cell selected mis-matched, related, allogeneic transplant will be assess in patients with relapsed/refractory neuroblastoma

  Monitoring of mortality, toxicity (NCI Common Criteria), acute and chronic graft versus host disease, engraftment rate will contribute to safety assessment

  6 weeks

Secondary Outcomes (1)

• Infusional and long term safety and persistence of tumor redirected, genetically modified, donor derived, allogeneic multi-virus specific cytotoxic T-cells (tV-CTL) after allogeneic hematopoietic stem cell transplant in patients with neuroblastoma

  4-8 weeks post transplant

Study Arms (1)

Single Arm

EXPERIMENTAL

Haploidentical allogeneic stem cell transplant following sub-myeloablative conditioning and cell selection using the Miltenyi Clinimacs device

Device: CD34+ cells selected with the Miltenyi Clinimacs machine

Interventions

CD34+ cells selected with the Miltenyi Clinimacs machine DEVICE

Haploidentical allogeneic stem cell transplant following sub-myeloablative conditioning and cell selection using the Miltenyi Clinimacs device

Single Arm

Eligibility Criteria

• Age: 6 Months - 17 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Child (0-17)

You may qualify if:

• Age 6 months - \<18 years
• Measurable tumor by routine imaging or bone marrow biopsy
• Patient must have an 3/6, 4/6, or 5/6 human leukocyte antigen (HLA)-mismatched related donor who is Epstein-Barr virus (EBV) seropositive
• Karnofsky score 60% or greater if 10yrs old or older, Lansky score 60% or greater if under 10yrs old
• Pulse ox \>90% on room air
• Recovered from toxic effects of prior chemotherapy
• Patient must not be pregnant
• Patient must be HIV negative
• Patient or responsible person must be able to understand and sign an informed consent
• Available donor without contraindication for stem cell collection

You may not qualify if:

• Pregnant and lactating women.
• Human immunodeficiency virus (HIV) positive patient.
• Uncontrolled intercurrent infection.
• Renal failure (Creatine \> 1.5 or Creatinine Clearance \< 40 ml/min/1.73m2)
• Active hepatitis or cirrhosis with liver test values greater than 3 times normal
• NOTE: Patients who would be excluded from the protocol strictly for laboratory abnormalities can be included at the investigator's discretion, after review by the Children's Mercy Hospital ethics board
• Donor must be in good health based on review of systems and results of physical examination, and routine testing per standards of good medical care.
• Female donors of childbearing age must have a negative pregnancy test and must not be lactating
• EBv seropositive
• Donor stem cells should be human leukocyte antigen (HLA) typed using molecular methods. See section 6.1.3 for HLA matching requirements.

Sponsors & Collaborators

• Children's Mercy Hospital Kansas City: lead
• University of Kansas: collaborator

Study Sites (1)

Children's Mercy Hospital

Kansas City, Missouri, 64108, United States

Location

Related Links

• Children's Mercy Hospital

MeSH Terms

Conditions

Neuroblastoma; Recurrence

Condition Hierarchy (Ancestors)

Neuroectodermal Tumors, Primitive, Peripheral; Neuroectodermal Tumors, Primitive; Neoplasms, Neuroepithelial; Neuroectodermal Tumors; Neoplasms, Germ Cell and Embryonal; Neoplasms by Histologic Type; Neoplasms; Neoplasms, Glandular and Epithelial; Neoplasms, Nerve Tissue; Disease Attributes; Pathologic Processes; Pathological Conditions, Signs and Symptoms

Study Officials

• Gary D Myers, MD

  Children's Mercy

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: phase 1
• Allocation: NA
• Masking: NONE
• Purpose: DEVICE FEASIBILITY
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: October 25, 2011
• First Posted: October 31, 2011
• Study Start: October 1, 2011
• Primary Completion: December 1, 2015
• Study Completion: December 1, 2015
• Last Updated: July 18, 2018

Record last verified: 2018-07

Locations

US (1)