The Effects of Concentration/Meditation on the Innate Immune Response During Human Endotoxemia

NCT01352871 | Completed DMC

• 1 other identifier: LPS-concentration
• Study Type: observational
• Target: 1
• Locations: 1 country
• Sites: 1

Brief Summary

The innate immune response is the first line of defense against invading pathogens. Ideally, the inflammatory response is tightly regulated leading to both adequate protection to invading pathogens as well as limitation of an exuberant or unwanted immune response such as seen in sepsis or auto-immune diseases. It has become increasingly clear that the autonomic nervous system (ANS) and the innate immune response are intimately linked. Activation of the sympathetic division of ANS dampens inflammation via β2-adrenoceptors. On the other hand, in some cases, sympathetic drive can also stimulate the inflammatory response via α2-adrenoceptors. The parasympathetic branch of the ANS modulates the inflammatory response as well, since it was discovered that electrical stimulation of the efferent vagus nerve in rats greatly inhibits the innate immune response. Generally, the ANS is regarded as pure autonomic which can not be influenced by behavior. However, trough special concentration/mediation techniques mastered by certain individuals, it might be possible to modulate ANS activity. In addition, recent unpublished findings indicate that these concentration/meditation techniques can also influence the inflammatory response ex vivo. In this study the investigators wish to investigate the effect of concentration/meditation on the innate immune response in vivo. In addition the investigators wish to elucidate the mechanism via which this effect is mediated. The investigators aim to use the so called human endotoxemia model. This model permits elucidation of key players in the immune response to a gram negative stimulus in vivo, therefore serving as a useful tool to investigate potential novel therapeutic strategies in a standardized setting. Objectives: Primary objective: The primary objective of the study is to determine the effect of concentration/meditation on the innate immune response induced by a lipopolysaccharide (LPS) challenge. Secondary Objective(s):

1. 1.To determine the effects of concentration/meditation on ANS activity. Electroencephalography (EEG), heart-rate variability (HRV), muscle sympathetic nerve activity and plasma concentrations of catecholamines will be measured for this purpose.
2. 2.To determine if concentration/meditation can attenuate (subclinical) renal damage known to occur during human endotoxemia, markers of proximal and distal tubular damage will be measured at various time points.

Conditions

Innate Immune Response

Keywords

Immunity, Innate; Attention; Concentration

Outcome Measures

Primary Outcomes (1)

• Change in Plasma TNF-alpha levels

  Concentration of circulating TNF-alfa at certain timepoints.

  0; 1; 1.5; 2; 3; 4; 6; 8; 12; 24 hrs after endotoxin administration

Secondary Outcomes (3)

• Change in plasma IL-6, IL-10 and IL-1ra levels and leukocyte counts

  0; 1; 1.5; 2; 3; 4; 6; 8; 12; 24 hrs after endotoxin administration

• Change in measures of autonomous nervous system activity

  at regulare intervals before and during endotoxemia

• Change in markers of subclinical renal tubular damage

  before and at 0-3, 3-6, 6-9, 9-12 and 12-24 hrs after endotoxemia

Study Arms (1)

Concentration / meditation

The subject will try to influence the innate immune response by concentration / meditation in advance of and during endotoxemia

Behavioral: Concentration / meditation; Drug: lipopolysaccharide

Interventions

Concentration / meditation BEHAVIORAL

from 30 minutes before endotoxin administration to 1,5 hrs after endotoxin administration the subject is concentrating / meditating with the goal to influence the innate immune response

Concentration / meditation

lipopolysaccharide DRUG

lipopolysaccharide 2ng/kg intravenously

Also known as: LPS, endotoxin

Concentration / meditation

Eligibility Criteria

• Age: 45 Years - 55 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

One healthy male volunteer that masters the concentration/meditation technique.

You may qualify if:

• years of age
• male
• Healthy

You may not qualify if:

• Use of any medication.
• Smoking.
• Bleeding disorder.
• Previous spontaneous vagal collapse.
• History, signs or symptoms of cardiovascular disease.
• Cardiac conduction abnormalities on the ECG consisting of a 2nd degree atrioventricular block or a complex bundle branch block.
• Hypertension (defined as RR systolic \> 160 or RR diastolic \> 90).
• Hypotension (defined as RR systolic \< 100 or RR diastolic \< 50).
• Renal impairment (defined as plasma creatinin \>120 μmol/l).
• Liver enzyme abnormalities or positive hepatitis serology.
• Positive HIV serology or any other obvious disease associated with immune deficiency.
• Febrile illness in the week before the LPS challenge.
• Participation in another drug trial or donation of blood 3 months prior to the planned LPS challenge.

Sponsors & Collaborators

• Radboud University Medical Center: lead

Study Sites (1)

Radboud University Nijmegen Medical Centre

Nijmegen, Gelderland, 6511HB, Netherlands

Location

MeSH Terms

Interventions

Meditation; Lipopolysaccharides; Endotoxins

Intervention Hierarchy (Ancestors)

Mind-Body Therapies; Complementary Therapies; Therapeutics; Spiritual Therapies; Relaxation Therapy; Behavior Therapy; Psychotherapy; Behavioral Disciplines and Activities; Glycoconjugates; Carbohydrates; Polysaccharides, Bacterial; Polysaccharides; Lipids; Antigens, Bacterial; Antigens; Biological Factors; Bacterial Toxins; Toxins, Biological

Study Officials

• Peter Pickkers, MD, PhD

  Radboud University Medical Center

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE ONLY
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER

Study Record Dates

• First Submitted: November 26, 2010
• First Posted: May 12, 2011
• Study Start: March 1, 2011
• Primary Completion: April 1, 2011
• Study Completion: May 1, 2011
• Last Updated: June 7, 2011

Record last verified: 2011-02

Locations

NL (1)