Expression Levels of RISC and Microprocessor Complex Components in Epithelial Skin Cancer

NCT01345760 | Completed DMC

• 1 other identifier: 1-Sand
• Study Type: observational
• Target: 44
• Locations: 1 country
• Sites: 1

Brief Summary

MicroRNAs (miRNAs) are very small endogenous RNA molecules about 22-25 nucleotides in length, capable of post-transcriptional gene regulation. miRNAs bind to their target messenger RNAs (mRNAs), leading to cleavage or suppression of target mRNA translation based on the degree of complementarity. miRNAs have recently been shown to play pivotal roles in diverse developmental and cellular processes and linked to a variety of skin diseases and cancers. In the present study, the investigators examines the expression profiles of the microprocessor complex subunit DGCR8 and the RISC components TARBP1, TARBP2, argonaute-1, argonaute-2 and PACT in epithelial skin cancer and its premalignant stage.

Conditions

Skin Cancer

Keywords

Basal Cell Carcinoma; Squamous Cell Carcinoma; Actinic Keratosis; microRNA; Dicer; Drosha; RISC; microprocessor complex; Argonaute; DGCR8; TARBP

Study Arms (4)

Basal Cell Carcinoma

Squamous Cell Carcinoma

Actinic Keratosis

healthy non-lesional skin

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Patients with epithelial skin cancer and pre-malignant stages

You may qualify if:

• epithelial skin cancer

You may not qualify if:

• other known previous malignancies

Sponsors & Collaborators

• Ruhr University of Bochum: lead

Study Sites (1)

Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum

Bochum, North Rhine-Westphalia, 44791, Germany

Location

Related Publications (1)

• Sand M, Skrygan M, Georgas D, Arenz C, Gambichler T, Sand D, Altmeyer P, Bechara FG. Expression levels of the microRNA maturing microprocessor complex component DGCR8 and the RNA-induced silencing complex (RISC) components argonaute-1, argonaute-2, PACT, TARBP1, and TARBP2 in epithelial skin cancer. Mol Carcinog. 2012 Nov;51(11):916-22. doi: 10.1002/mc.20861. Epub 2011 Oct 24.

  PMID: 22025453 RESULT

Biospecimen

Retention: SAMPLES WITH DNA

human skin

MeSH Terms

Conditions

Skin Neoplasms; Carcinoma, Basal Cell; Carcinoma, Squamous Cell; Keratosis, Actinic; Rhabdomyosarcoma, Embryonal, 2

Condition Hierarchy (Ancestors)

Neoplasms by Site; Neoplasms; Skin Diseases; Skin and Connective Tissue Diseases; Carcinoma; Neoplasms, Glandular and Epithelial; Neoplasms by Histologic Type; Neoplasms, Basal Cell; Neoplasms, Squamous Cell; Precancerous Conditions; Keratosis

Study Officials

• Falk G Bechara, PD Dr.

  Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum

  STUDY DIRECTOR

Study Design

• Study Type: observational
• Observational Model: CASE ONLY
• Time Perspective: RETROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Dr.

Study Record Dates

• First Submitted: April 29, 2011
• First Posted: May 2, 2011
• Study Start: July 1, 2008
• Primary Completion: February 1, 2011
• Study Completion: February 1, 2011
• Last Updated: October 16, 2012

Record last verified: 2012-10

Locations

DE (1)