NCT01317745

Brief Summary

Approximately 216, and up to 270, healthy males and non-pregnant females, 18 to 49 years old, inclusive, will be enrolled over a 5-month period into this multicenter, randomized, double-blinded, controlled Phase I study. Subjects who meet the entry criteria for the study and provide informed consent will be randomized 2:1 between adjuvanted and unadjuvanted vaccine and placed into one of 6 groups (see table) to receive two doses of an intramuscular subvirion inactivated monovalent influenza A/H5N1 virus vaccine at 3.75, 7.5, or 15 mcg given with the adjuvant MF59 or diluent (N=216, up to 270). All eligible subjects will receive 2 doses separated by approximately 21 days.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
270

participants targeted

Target at P75+ for phase_1

Timeline
Completed

Started May 2011

Geographic Reach
1 country

5 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

March 16, 2011

Completed
1 day until next milestone

First Posted

Study publicly available on registry

March 17, 2011

Completed
2 months until next milestone

Study Start

First participant enrolled

May 1, 2011

Completed
1.3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

September 1, 2012

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

September 1, 2012

Completed
Last Updated

January 25, 2013

Status Verified

August 1, 2012

Enrollment Period

1.3 years

First QC Date

March 16, 2011

Last Update Submit

January 24, 2013

Conditions

Keywords

H5N1, vaccine, influenza

Outcome Measures

Primary Outcomes (3)

  • Immunogenicity: Geometric mean titer (GMT) of HAI antibody, subjects with HAI antibody titer 1:40 or greater, and frequency of 4-fold or greater HAI antibody increase against A/H5N1 vaccine in each group 21 days following 2nd vaccine dose (approx Day 42)

    Day 42

  • Safety/Tolerability: Occurrence of vaccine-associated serious adverse events (SAEs) and clinical safety laboratory adverse events (AEs) from the time of first vaccination through 13 months after the first vaccination.

    From time of 1st vaccination through 13 months

  • Safety/Tolerability: Occurrence of solicited local and systemic reactogenicity in the 8 days (Day 0-7) after each vaccination.

    Day 0 through Day 7 after each vaccination

Secondary Outcomes (1)

  • GMT of neutralizing antibody (NA), proportion of subjects with a serum NA titer of 1:40 or greater, and frequency of 4-fold or greater increases of NA against A/H5N1 virus vaccine in each group 21 days following 2nd dose of vaccine (approximately Day 42)

    Day 42

Study Arms (6)

Group 5

PLACEBO COMPARATOR

Two doses of sanofi H5N1 antigen 7.5 mcg plus PBS diluent

Other: Phosphate Buffered Saline (PBS) diluentBiological: H5N1 Antigen

Group 4

PLACEBO COMPARATOR

Two doses of sanofi H5N1 antigen 3.75 mcg plus Phosphate Buffered Saline (PBS) diluent

Other: Phosphate Buffered Saline (PBS) diluentBiological: H5N1 Antigen

Group 3

EXPERIMENTAL

Two doses of sanofi H5N1 antigen 15 mcg plus Novartis MF59 adjuvant

Drug: MF59 AdjuvantBiological: H5N1 Antigen

Group 2

EXPERIMENTAL

Two doses of sanofi H5N1 antigen 7.5 mcg plus Novartis MF59 adjuvant

Drug: MF59 AdjuvantBiological: H5N1 Antigen

Group 1

EXPERIMENTAL

Two doses of sanofi H5N1 antigen 3.75 mcg plus Novartis MF59 adjuvant

Drug: MF59 AdjuvantBiological: H5N1 Antigen

Group 6

PLACEBO COMPARATOR

Two doses of sanofi H5N1 antigen 15 mcg plus PBS diluent

Other: Phosphate Buffered Saline (PBS) diluentBiological: H5N1 Antigen

Interventions

Placebo/diluent provided be sanofi pasteur

Group 4Group 5Group 6

Adjuvant/diluent manufactured by Novartis

Group 1Group 2Group 3
H5N1 AntigenBIOLOGICAL

Subvirion inactivated monovalent influenza A/H5N1 (Hemagglutinin (HA) of A/Indonesia/05/2005) virus vaccine manufactured by sanofi pasteur, at 2 doses levels 21 days apart, , administered at 3.75, 7.5, or 15mcg, with either MF59 adjuvant or PBS.

Group 1Group 2Group 3Group 4Group 5Group 6

Eligibility Criteria

Age18 Years - 49 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Are males or non-pregnant females between the ages of 18 and 49 years, inclusive.
  • Women of childbearing potential (not surgically sterile via tubal ligation, bilateral oophorectomy or hysterectomy or who are not postmenopausal for \>/=1 year) must agree to practice adequate contraception (that may include, but is not limited to, abstinence, monogamous relationship with vasectomized partner, barrier methods such as condoms or diaphragms with spermicide or foam, intrauterine devices, and licensed hormonal methods) during the study for at least 30 days following the last vaccination. Adherence to contraceptive method will be captured on the appropriate case report form (CRF).
  • Are in good health, as determined by vital signs (oral temp, pulse and blood pressure), medical history and complete physical examination (without genital and rectal exam) to ensure no existing chronic medical diagnoses or conditions are present.
  • ESR less than 30 mm per hour.
  • For women of childbearing potential, negative urine or serum pregnancy test within 24 hours prior to the first vaccination.
  • Are able to understand and comply with planned study procedures.
  • Provide written informed consent prior to initiation of any study procedures.
  • Have received the first dose of study vaccine.
  • For women of childbearing potential, negative urine or serum pregnancy test within 24 hours prior to the second vaccination. Adherence to contraceptive method will be captured on the appropriate case report form (CRF).

You may not qualify if:

  • Have a known allergy to eggs or other components of the vaccine (including gelatin, formaldehyde, octoxinol-9, thimerosal and chicken protein), or allergy to squalene-based adjuvants.
  • Women who are breastfeeding or plan to breastfeed at any given time from the first vaccination until 30 days after the last vaccination.
  • Have long term use (defined as taken for 2 weeks or more in total at any time during the past 2 months) of high dose oral or parenteral glucocorticoids (high dose defined as prednisone \>/= 20 mg total daily dose, or equivalent dose of other glucocorticoids); or high-dose inhaled steroids (high dose defined as \>800 mcg/day of beclomethasone dipropionate or equivalent); or systemic corticosteroids of any dose within the past 4 weeks.
  • Have immunosuppression as a result of an underlying illness or treatment, or use of anticancer chemotherapy or radiation therapy (cytotoxic) within the preceding 36 months.
  • Have an active neoplastic disease or a history of any hematologic malignancy.
  • Have a diagnosis of schizophrenia, bipolar disease, or other major psychiatric diagnosis.
  • Hospitalized for psychiatric illness, history of suicide attempt, or confinement for danger to self or others, within the past 10 years.
  • Receiving systemic, prescription medications for the treatment of chronic medical conditions, unless such use is on a PRN (as needed) basis only. Non-PRN use of systemic, over-the-counter medications and PRN systemic, prescription medication may be allowed if, in the opinion of the investigator, they pose no additional risk to subject safety or assessment of immunogenicity/reactogenicity. Note: Topical, nasal, and inhaled medications; vitamins; and contraceptives are also permitted.
  • Received pre-medication with analgesic or antipyretic agents in the 6 hours prior to first vaccination, or planned medication with analgesic or antipyretic in the week following first vaccination. This criterion should not preclude subjects receiving such medication if the need arises. However, pre-medication is to be discouraged.
  • Received immunoglobulin or other blood products (with exception of Rho D immune globulin) within the 3 months prior to the first vaccination..
  • Received any live licensed vaccines within 4 weeks or inactivated licensed vaccines within 2 weeks prior to the first vaccination or plan receipt of such vaccines within 42 days following the first vaccination. This is inclusive of licensed seasonal influenza vaccines.
  • Have an acute or chronic medical condition that, in the opinion of the site principal investigator or appropriate sub-investigator, would render vaccination unsafe, would interfere with the evaluation of responses or is not generally seen in "normal, healthy subjects".
  • Have a history of severe reactions following previous immunization with contemporary influenza virus vaccines.
  • Have an acute illness, including an oral temperature greater than or equal to 100.4 degrees F, within 3 days prior to the first vaccination.
  • Pulse is less than 40 bpm or greater than 100 bpm. An ECG documenting only sinus bradycardia is required for pulses less than 50 bpm.
  • +31 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (5)

Emory Children's Center - Division of Pediatric Infectious Diseases

Atlanta, Georgia, 30322, United States

Location

Emory Vaccine Center - The Hope Clinic

Decatur, Georgia, 30030-2517, United States

Location

University of Iowa - Infectious Disease Clinic

Iowa City, Iowa, 52242-1009, United States

Location

Saint Louis University - Center for Vaccine Development

St Louis, Missouri, 63104-1015, United States

Location

Cincinnati Children's Hospital Medical Center - Infectious Diseases

Cincinnati, Ohio, 45229-3026, United States

Location

MeSH Terms

Conditions

Influenza, HumanInfluenza in Birds

Condition Hierarchy (Ancestors)

Respiratory Tract InfectionsInfectionsOrthomyxoviridae InfectionsRNA Virus InfectionsVirus DiseasesRespiratory Tract DiseasesBird DiseasesAnimal Diseases

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, INVESTIGATOR
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
NIH
Responsible Party
SPONSOR

Study Record Dates

First Submitted

March 16, 2011

First Posted

March 17, 2011

Study Start

May 1, 2011

Primary Completion

September 1, 2012

Study Completion

September 1, 2012

Last Updated

January 25, 2013

Record last verified: 2012-08

Locations