NCT01278940

Brief Summary

PRIMARY OBJECTIVES: Determination of safety and toxicity of vaccination with patients' tumour mRNA transfected DCs . SECONDARY OBJECTIVES:Determine immunological response to the vaccine (induction of specific T-cell response) and assessment of tumour response

Trial Health

100
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
31

participants targeted

Target at P25-P50 for phase_1

Timeline
Completed

Started Mar 2002

Longer than P75 for phase_1

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

March 1, 2002

Completed
3.9 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

February 1, 2006

Completed
5 years until next milestone

First Submitted

Initial submission to the registry

January 17, 2011

Completed
2 days until next milestone

First Posted

Study publicly available on registry

January 19, 2011

Completed
4.9 years until next milestone

Study Completion

Last participant's last visit for all outcomes

December 1, 2015

Completed
Last Updated

August 15, 2016

Status Verified

August 1, 2016

Enrollment Period

3.9 years

First QC Date

January 17, 2011

Last Update Submit

August 12, 2016

Conditions

Outcome Measures

Primary Outcomes (1)

  • Determination of safety and toxicity of vaccination with patients' tumour mRNA transfected DCs

    Biochemistry and hematology results, vital signs and ECOG performance status are measured at those timepoints.

    Patients are coming every week during 6 weeks.

Secondary Outcomes (2)

  • Determine immunological response to the vaccine (induction of specific T-cell response)

    6 weeks and 3 months after study start

  • Assessment of tumour response.

    3 months after study start

Study Arms (2)

Dendritic Cells (DC) malignant melanoma

EXPERIMENTAL

22 patients were included in this arm.

Biological: Dendritic Cells (DC) malignant melanoma

DC vaccine plus IL-2

EXPERIMENTAL

To improve the efficacy of the DC vaccine, IL-2 was administrated at the vaccination site through direct lymph node injection. 9 patients were included in this arm.

Biological: Dendritic Cells (DC) malignant melanomaProcedure: IL-2

Interventions

The patients were assigned to intradermal or intranodal DC vaccination

DC vaccine plus IL-2Dendritic Cells (DC) malignant melanoma
IL-2PROCEDURE

IL-2 were administrated by intranodal injection

DC vaccine plus IL-2

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Accessible tumour tissue for vaccine production (extraction of tumour mRNA) i.e.subcutaneous or lymph node metastases.
  • Must be at least 18 years of age.
  • Must have histologically confirmed advanced, metastatic cutaneous melanoma no longer amenable for surgery.
  • Must have evidence of disease progression and measurable or evaluable metastases
  • Must be ambulatory with a ECOG performance score of \<2
  • Must have lab.values as following :
  • ANC \> 1.5 x 109/L; platelets \> 100 x 109/L, Hb \> 9g/dL (\> 5.6 mmol/L). Creatinine \< 140 µmol/L (1.6 mg/dL); if borderline, the creatinine clearance \> 40 mL/min, Bilirubin \< 20% above the upper limit of normal, ASAT and ALAT \< 2.5 the upper limit of normal. Albumin \> 2.5 g/L.
  • Prior radiotherapy: A minimum of 4 weeks (8 weeks in case of extensive radiotherapy) must have elapsed between the end of the prior radiotherapy and entry into the protocol.
  • Prior chemotherapy: A minimum 4 weeks must have elapsed between the end of the prior chemotherapy and entry into the protocol.
  • Signed informed consent of the patients for the treatment and follow up must be obtained and documented according to the ICH-GCP Guidelines.

You may not qualify if:

  • History of prior malignancy other than melanoma, with the exception of curatively treated basal cell or squamous cell carcinoma of the skin and ca. cervix stage 1B.
  • Active infection requiring antibiotic therapy.
  • Significant cardiac or other medical illness that would limit activity or survival, such as severe congestive heart failure, unstable angina, or serious cardiac arrhythmia.
  • Autoimmune disease currently treated with steroids.
  • Adverse reactions to vaccines such as anaphylaxis or other serious reactions.
  • History of immunodeficiency or autoimmune disease such as rheumatoid arthritis, systemic lupus erythematosus, scleroderma, polymyositis-dermatomyositis, juvenile onset insulin dependent diabetes, or a vasculitic syndrome.
  • Chemotherapy or other potentially immune-suppressive therapy that has been administered within 4 weeks prior to vaccination.
  • Pregnancy or lactation.
  • Any reason why, in the opinion of the investigator, the patient should not participate.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Related Publications (2)

  • Kyte JA, Mu L, Aamdal S, Kvalheim G, Dueland S, Hauser M, Gullestad HP, Ryder T, Lislerud K, Hammerstad H, Gaudernack G. Phase I/II trial of melanoma therapy with dendritic cells transfected with autologous tumor-mRNA. Cancer Gene Ther. 2006 Oct;13(10):905-18. doi: 10.1038/sj.cgt.7700961. Epub 2006 May 5.

  • Kyte JA, Kvalheim G, Lislerud K, thor Straten P, Dueland S, Aamdal S, Gaudernack G. T cell responses in melanoma patients after vaccination with tumor-mRNA transfected dendritic cells. Cancer Immunol Immunother. 2007 May;56(5):659-75. doi: 10.1007/s00262-006-0222-y. Epub 2006 Sep 1.

MeSH Terms

Conditions

Melanoma

Interventions

Interleukin-2

Condition Hierarchy (Ancestors)

Neuroendocrine TumorsNeuroectodermal TumorsNeoplasms, Germ Cell and EmbryonalNeoplasms by Histologic TypeNeoplasmsNeoplasms, Nerve TissueNevi and MelanomasSkin NeoplasmsNeoplasms by SiteSkin DiseasesSkin and Connective Tissue Diseases

Intervention Hierarchy (Ancestors)

InterleukinsCytokinesIntercellular Signaling Peptides and ProteinsPeptidesAmino Acids, Peptides, and ProteinsLymphokinesProteinsBiological Factors

Study Officials

  • Steinar Aamdal, M.D PhD Prof

    Oslo University Hospital - Norwegian Radium Hospital

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
phase 1
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
MD, PhD

Study Record Dates

First Submitted

January 17, 2011

First Posted

January 19, 2011

Study Start

March 1, 2002

Primary Completion

February 1, 2006

Study Completion

December 1, 2015

Last Updated

August 15, 2016

Record last verified: 2016-08