NCT01120743

Brief Summary

The primary goal of this project is to identify the source of cytokines that are released into circulation during graft reperfusion. Seventeen patients scheduled to have adult cadaveric liver transplantation at the Milton S. Hershey Medical Center were contacted as prospective participants. Blood samples were obtained from the radial artery, the portal vein, and from the graft irrigation. The level of pro-inflammatory cytokines was verified and compared with the amount of catecholamines used to maintain hemodynamic stability.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
17

participants targeted

Target at below P25 for all trials

Timeline
Completed

Started Aug 2008

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

August 1, 2008

Completed
1.7 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 1, 2010

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

April 1, 2010

Completed
1 month until next milestone

First Submitted

Initial submission to the registry

May 3, 2010

Completed
8 days until next milestone

First Posted

Study publicly available on registry

May 11, 2010

Completed
Last Updated

November 24, 2017

Status Verified

November 1, 2017

Enrollment Period

1.7 years

First QC Date

May 3, 2010

Last Update Submit

November 21, 2017

Conditions

Keywords

liver failurecytokineshemodynamic instability

Outcome Measures

Primary Outcomes (1)

  • Measurement of cytokine levels (TNF-alpha, IL-1, Il-2, IL-6, IL-8) in the portal vein, radial artery and "flush" (from irrigation of the liver used to remove preservation solution from the liver graft) blood.

    Cytokines released from the liver graft could be a cause for negative inotropy and systemic vasodilatation.

    A period of 20 minutes, beginning at the start of reperfusion and continuing until 20 minutes after reperfusion.

Secondary Outcomes (1)

  • Correlation between the level of cytokines and hemodynamic stability during reperfusion.

    A period of 20 minutes, beginning at the start of reperfusion and continuing until 20 minutes after reperfusion.

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Study will include adult patients with end-stage liver disease accepted for liver transplantation.

You may qualify if:

  • All adult patients scheduled for liver transplantation will be offered the opportunity to participate in this research.

You may not qualify if:

  • Patients unable or unwilling to provide adequate informed consent will be excluded.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Penn State Milton S. Hershey Medical Center, Penn State College of Medicine

Hershey, Pennsylvania, 17033-0850, United States

Location

Related Publications (1)

  • Bezinover D, Kadry Z, McCullough P, McQuillan PM, Uemura T, Welker K, Mastro AM, Janicki PK. Release of cytokines and hemodynamic instability during the reperfusion of a liver graft. Liver Transpl. 2011 Mar;17(3):324-30. doi: 10.1002/lt.22227.

Biospecimen

Retention: SAMPLES WITHOUT DNA

Blood samples were obtained. All blood samples were placed on ice and processed with centrifugation. The plasma supernatant was frozen at -80º Celsius.

MeSH Terms

Conditions

Liver Failure

Condition Hierarchy (Ancestors)

Hepatic InsufficiencyLiver DiseasesDigestive System Diseases

Study Officials

  • Dmitri Bezinover, MD, PhD

    Penn State Milton S. Hershey Medical Center, Penn State College of Medicine

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

May 3, 2010

First Posted

May 11, 2010

Study Start

August 1, 2008

Primary Completion

April 1, 2010

Study Completion

April 1, 2010

Last Updated

November 24, 2017

Record last verified: 2017-11

Locations