Expansion of Umbilical Cord Blood Using a Unique Bio-system

NCT01061879 | Completed

• 1 other identifier: 11973
• Study Type: observational
• Target: 8
• Locations: 1 country
• Sites: 1

Brief Summary

By doing this study, researchers hope to develop a special technique and laboratory conditions to help support umbilical stem cell growth and multiplication. In this project, researchers propose a three dimensional (3D) microenvironment that mimics the bone marrow stem cell microenvironment, with its supporting cells of osteoblasts and stromal cells. In theory, this will result in better expansion of cord blood stem cells ex vivo.

Conditions

Pregnancy

Keywords

umbilical cord; umbilical cord blood collection; pregnancy

Outcome Measures

Primary Outcomes (1)

• bio-system for ex-vivo expansion of umbilical cord blood stem cells

  To successfully expand the umbilical cord blood stem cells, a microenvironment that resembles the stem cell microenvironment, or stem cell 'niche' should be created. In designing this bio-system, we make use of several observations. Firstly, bone osteoblasts are important in promoting hematopoietic stem cell expansion , accordingly, this bio-system will ensure the physical proximity of the cord blood stem cells to a 3D bone tissue derived from mesenchymal stem cells (MSCs). These MSCs will be isolated from Wharton's jelly, and by using an osteogenic medium and special scaffolds, these MSCs will differentiate into osteogenic progenitors creating a 3D bone structure . The other observation is that, co-culture of cord blood stem cells with mesenchymal stem cells is superior to liquid cultures in terms of ex vivo expansion , accordingly, in this experiment, undifferentiated MSCs will be co-cultured with cord blood stem cells in the created 3D bone structure.

  one year

Eligibility Criteria

• Age: 18 Years+
• Sex: female
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

umbilical cord collection from full-term caesarian section/normal vaginal delivery patients

You may qualify if:

• females that consent to permit researchers collect the discarded umbilical cords from the placenta and use for research purposes.

Sponsors & Collaborators

• University of Kansas: lead

Study Sites (1)

University of Kansas Medical Center

Kansas City, Kansas, 66160, United States

Location

Biospecimen

Retention: SAMPLES WITH DNA

umbilical cord

Study Officials

• Omar Aljitawi, MD

  University of Kansas Medical Center

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: February 2, 2010
• First Posted: February 3, 2010
• Study Start: February 1, 2010
• Primary Completion: November 1, 2010
• Study Completion: November 1, 2010
• Last Updated: January 3, 2013

Record last verified: 2012-12

Locations

US (1)