Rijavithi Viral and Mycobacterium PCR Arrays in CNS Infection Study

NCT00807378 | Completed DMC

• 1 other identifier: RH-CMR-004
• Study Type: observational
• Target: 200
• Locations: 0 countries
• Sites: N/A

Brief Summary

This study of neurotropic viral infection and Tuberculous CNS infection is the comprehensive investigation of CSF PCR arrays (Hybrydiaztion technique,Sybergreen and Taqman real time PCR arrays of CSF ) in suspected cases of viral CNS infection, Tuberculous CNS infection in both AIDS and non AIDS patients. Patients with clinical viral encephalitis and all meningoencephalitis including all patients with HIV/AIDS CSF and non AIDS will be banked and test for RNA and DNA viral sequence by Real time PCR arrays and IS6110 gene of Mycobacterium tuberculosis. Clinical data and final diagnosis will be used to analyze the result of this PCR arrays technique compared to clinical gold standard criteria.

Conditions

CNS Viral Infection

Keywords

CNS viral infection; PCR; HYBRIDIZATION

Study Arms (2)

1

AIDS PATIENTS

Other: LUMBAR PUNCTURE

2

NON-AIDS PATIENTS

Other: LUMBAR PUNCTURE

Interventions

LUMBAR PUNCTURE OTHER

LUMBAR PUNCTURE FFOR CSF COLLECTION

1; 2

Eligibility Criteria

• Age: 15 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

CNS VIRAL INFECTION AIDS/NON-AIDS

You may qualify if:

• AGE MORE THAN 15 YRS
• ABLE TO INFORM CONSENT BY THEMSELF OR THEIR PARENT
• SUSPECTED CNS VIRAL INFECTION

You may not qualify if:

• UNABLE/CONTRAINDICATED FOR LUMBAR PUNCTURE OR CSF COLLECTION
• WITHDRAW CONSENT
• SUSPECTED SEVERE OR VILURENT VIRAL INFECTION SUCH AS H5N1/SARS INFECTION

Sponsors & Collaborators

• Department of Medical Services Ministry of Public Health of Thailand: lead
• Rajavithi Biomolecular Research Center: collaborator

Biospecimen

Retention: SAMPLES WITH DNA

CSF DNA/RNA virus

MeSH Terms

Conditions

Central Nervous System Viral Diseases

Interventions

Spinal Puncture

Condition Hierarchy (Ancestors)

Central Nervous System Infections; Infections; Virus Diseases; Central Nervous System Diseases; Nervous System Diseases

Intervention Hierarchy (Ancestors)

Biopsy; Specimen Handling; Clinical Laboratory Techniques; Diagnostic Techniques and Procedures; Diagnosis; Diagnostic Techniques, Neurological; Punctures; Therapeutics; Surgical Procedures, Operative; Investigative Techniques

Study Design

• Study Type: observational
• Observational Model: CASE ONLY
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER GOV

Study Record Dates

• First Submitted: December 9, 2008
• First Posted: December 11, 2008
• Study Start: July 1, 2008
• Primary Completion: December 1, 2009
• Study Completion: March 1, 2010
• Last Updated: May 20, 2010

Record last verified: 2010-05