Influence of Diesel Exhaust Particles (DEP) With and Without Organic Compounds on the Allergic Inflammation in Asthmatic Subjects

NCT00773487 | Completed

• 1 other identifier: 08/06 DEP-SFB
• Study Type: interventional
• Target: 15
• Locations: 1 country
• Sites: 1

Brief Summary

The primary objective of this study is to elucidate the effects of Diesel particles on the allergic inflammation in the airways of asthmatic patients.

Conditions

Asthma

Keywords

Asthma GINA 1; Mild intermittant asthma

Outcome Measures

Primary Outcomes (1)

• difference of the total number of eosinophils resulting in the comparison of allergic versus allergic inflammation in combination with Diesel particles with and without the organic fraction.

  24 post allergen challenge

Interventions

Bonchoscopy OTHER

Segmental instillation of allergen/Diesel particles; bronchoalveolar lavage

Eligibility Criteria

• Age: 18 Years - 55 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64)

You may qualify if:

• Able and willing to give written informed consent
• Physician diagnosis of mild intermittent or mild persistent asthma according to GINA guidelines {Bateman, 2008 34 /id}
• Age 18-55 years
• Nonsmokers with a history of less than 1 packyear having been nonsmokers for at least the last five years
• FEV1 \>= 80% of predicted, FEV1/FVC \>= 70 %
• Positive skin prick test for grass mix or D. pteronyssinus at or within 12 months prior to the screening visit
• Positive response to inhaled methacholine (\<= 8mg/ml)
• Positive response to an incremental inhaled allergen challenge with house dust mite allergen or grass pollen allergen
• Available to complete all study measurements
• Not pregnant, as confirmed by pregnancy test, and not nursing
• Of non-child bearing potential (i.e. physiologically incapable of becoming pregnant, including any female who is pre-menarchial or post-menopausal, with documented proof of hysterectomy or tubal ligation, or meet clinical criteria for menopause and has been amenorrhoeic for more than 1 year prior to the screening visit)
• Of childbearing potential and using a highly effective method of contraception during the entire study (vasectomised partner, sexual abstinence - the lifestyle of the female should be such that there is complete abstinence from intercourse from two weeks prior to the study until at least 72 hours after the end of the study -, implants, injectables, combined oral contraceptives, hormonal IUDs)

You may not qualify if:

• History of upper or lower respiratory tract infection four weeks prior to the informed consent visit
• Past or present disease, which as judged by the investigator, may affect the outcome of this study. These diseases include, but are not limited to, cardiovascular disease, malignancy, hepatic disease, renal disease, hematological disease, neurological disease, psychiatric disease, endocrine disease, infectious disease, inflammatory disease, or pulmonary disease (including but not confined to chronic bronchitis, emphysema, tuberculosis, bronchiectasis or cystic fibrosis).
• Clinically significant pathological findings in safety laboratory tests
• Regular intake of any medication other than short acting inhaled beta agonists, paracetamol for pain relief, birth control medication, hormonal replacement therapy, dietary and vitamin supplements
• Specific Immunotherapy (SIT) within two years prior to the study
• Use of anti-inflammatory medication including systemic or inhaled corticosteroid within the last four weeks
• Any history of life-threatening asthma, defined as an asthma episode that required intubation and/or was associated with hypercapnea, respiratory arrest or hypoxic seizures.
• Suspected hypersensitivity to any ingredients of the medication in line with bronchoscopy (bronchodilators, sedatives and local anesthetics)
• Conditions or factors, which would make the subject unlikely to be able to undergo bronchoscopy or incremental allergen challenge
• History of drug or alcohol abuse
• Suspected inability to understand the protocol requirements, instructions and study-related restrictions, the nature, scope, and possible consequences of the study.
• Participation in a clinical trial 30 days prior to enrolment
• Segmental allergen challenge 3 months prior to treatment
• Risk of non-compliance with study procedures

Sponsors & Collaborators

• Fraunhofer-Institute of Toxicology and Experimental Medicine: lead
• German Research Foundation: collaborator

Study Sites (1)

Fraunhofer ITEM

Hanover, Lower Saxony, 30625, Germany

Location

MeSH Terms

Conditions

Asthma

Condition Hierarchy (Ancestors)

Bronchial Diseases; Respiratory Tract Diseases; Lung Diseases, Obstructive; Lung Diseases; Respiratory Hypersensitivity; Hypersensitivity, Immediate; Hypersensitivity; Immune System Diseases

Study Officials

• Norber Krug, MD

  Fraunhofer ITEM

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER

Study Record Dates

• First Submitted: October 14, 2008
• First Posted: October 16, 2008
• Study Start: October 1, 2008
• Primary Completion: July 1, 2009
• Study Completion: July 1, 2009
• Last Updated: July 28, 2009

Record last verified: 2009-07

Locations

DE (1)