The Influence of Different Concentrations of Glucose in Culture Media on Fertilization and Embryo Quality
1 other identifier
interventional
100
0 countries
N/A
Brief Summary
The aim of this study is to examine whether incubation of human oocytes until pronuclear stage, in different culture media containing high and low concentrations of glucose affect fertilization differently, not only in standard IVF cycles, but also after intracytoplasmatic sperm injection.In addition, we wish to determine whether exposure of oocytes to different concentrations of glucose until pronuclear stage has further effect on embryo quality and development until the blastocyst stage.
Trial Health
Trial Health Score
Automated assessment based on enrollment pace, timeline, and geographic reach
participants targeted
Target at P50-P75 for not_applicable
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Trial Relationships
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Study Timeline
Key milestones and dates
First Submitted
Initial submission to the registry
January 21, 2008
CompletedFirst Posted
Study publicly available on registry
February 1, 2008
CompletedStudy Start
First participant enrolled
February 1, 2008
CompletedPrimary Completion
Last participant's last visit for primary outcome
August 1, 2009
CompletedFebruary 1, 2008
January 1, 2008
1.5 years
January 21, 2008
January 21, 2008
Conditions
Outcome Measures
Primary Outcomes (1)
Fertilization rate and embryo quality
6 days
Study Arms (4)
I
ACTIVE COMPARATORIVF Procedure-IVF Medium
II
ACTIVE COMPARATORIVF Procedure - ISM1 Medium
III
ACTIVE COMPARATORICSI Procedure - IVF Medium
IV
ACTIVE COMPARATORICSI Procedure - ISM1 Medium
Interventions
Sibling oocytes will be equally divided and incubated separately in different 4-well culture dishes containing either IVF medium or ISM1 Medium and while ICSI will be returned to the same culture dish following injection. Fertilization assessment will be performed approximately after 15-20 hours for standard IVF and 12-18 hours post ICSI performance. After assessment, fertilized oocytes from different culture media will be transferred separately into fresh culture dishes containing ISM1 medium only. The zygotes will be incubated until embryo transfer on day 2 or 3. Embryo quality will be assessed according to the morphology of blastomeres as well as it's developmental stage.
Eligibility Criteria
You may qualify if:
- Patients undergoing IVF treatment in our clinic during study period
You may not qualify if:
- Females older than 40 years
- Patients with less than 6 retrieved oocytes
- Male patients older than 50 years old
- Patients after testis biopsies
Contact the study team to confirm eligibility.
Sponsors & Collaborators
Study Design
- Study Type
- interventional
- Phase
- not applicable
- Masking
- NONE
- Purpose
- TREATMENT
- Intervention Model
- PARALLEL
- Sponsor Type
- OTHER GOV
Study Record Dates
First Submitted
January 21, 2008
First Posted
February 1, 2008
Study Start
February 1, 2008
Primary Completion
August 1, 2009
Last Updated
February 1, 2008
Record last verified: 2008-01