NCT00536120

Brief Summary

The primary objectives of this study were: to evaluate the effect of Tysabri® (natalizumab) on antibody responses after immunization with a neoantigen (keyhole limpet hemocyanin \[KLH\]) and a recall antigen (tetanus toxoid \[Td\]), and to evaluate the effect of Tysabri on circulating lymphocyte subsets (CD3+, CD4+, CD8+, CD19+, and CD56+) over time in participants with relapsing forms of multiple sclerosis (MS). The secondary objective was to assess alpha4-integrin saturation and alpha4-integrin expression levels over time.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
60

participants targeted

Target at P50-P75 for phase_4 multiple-sclerosis

Timeline
Completed

Started Jan 2008

Geographic Reach
1 country

10 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

September 25, 2007

Completed
2 days until next milestone

First Posted

Study publicly available on registry

September 27, 2007

Completed
3 months until next milestone

Study Start

First participant enrolled

January 1, 2008

Completed
1.8 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

November 1, 2009

Completed
1 month until next milestone

Study Completion

Last participant's last visit for all outcomes

December 1, 2009

Completed
1.7 years until next milestone

Results Posted

Study results publicly available

July 29, 2011

Completed
Last Updated

February 15, 2017

Status Verified

December 1, 2016

Enrollment Period

1.8 years

First QC Date

September 25, 2007

Results QC Date

March 17, 2011

Last Update Submit

December 29, 2016

Conditions

Keywords

MSMultiple sclerosisRelapsing forms of Multiple Sclerosis

Outcome Measures

Primary Outcomes (2)

  • Percentage of Keyhole Limpet Hemocyanin (KLH) Responders at Day 28 Post-Vaccination

    KLH responders were defined as those participants who had at least a 2-fold increase over pre-immunization level of anti-KLH antibodies in their blood at 28 days after vaccination with KLH.

    28 days after immunization (Day 28 for Vaccinations Only Group/Day 196 for Tysabri Plus Vaccinations Group)

  • Percentage of Tetanus Diphtheria Toxoid (Td) Responders at Day 28 Post-Vaccination

    Tetanus responders were defined as participants who had at least a 2-fold increase over pre-immunization levels of anti-tetanus antibodies in their blood at 28 days after they were immunized with tetanus.

    28 days after immunization (Day 28 for Vaccinations Only Group/Day 196 for Tysabri Plus Vaccinations Group)

Secondary Outcomes (4)

  • Mean Percentage Change From Baseline in Circulating Lymphocyte Subsets CD3+, CD4+, CD8+, CD19+, and CD56+ at Month 3 of Tysabri Therapy

    Month 0 (Baseline), Month 3

  • Mean Percentage Change From Baseline in Circulating Lymphocyte Subsets CD3+, CD4+, CD8+, CD19+, and CD56+ at Month 6 of Tysabri Therapy

    Month 0 (Baseline), Month 6

  • Mean Alpha4-Integrin Saturation at Baseline, Month 3, and Month 6

    Month 0 (Baseline), Month 3, and Month 6

  • Mean Alpha4-Integrin Expression at Baseline, Month 3, and Month 6

    Month 0 (Baseline), Month 3, and Month 6

Study Arms (2)

Tysabri Plus Vaccinations

EXPERIMENTAL

Participants receive 9 monthly doses of Tysabri 300 mg intravenous (IV), and receive vaccinations with neoantigen and recall antigen (keyhole limpet hemocyanin \[KLH\] and tetanus diphtheria toxoid \[Td\], according to manufacturer's prescribing information) at Month 6 (following the 7th dose of Tysabri) for both KLH and Td, and 14 and 28 days later for KLH.

Biological: BG00002 (natalizumab)Biological: keyhole limpet hemocyanin (KLH)Biological: tetanus diphtheria toxoid vaccine (Td)

Vaccinations Only

OTHER

Participants receive only vaccinations with neoantigen and recall antigen (KLH and Td, according to manufacturer's prescribing information) at Month 0 for both KLH and Td, and 14 and 28 days later for KLH. They do not receive any treatment for their MS and remain in the study through Month 2.

Biological: keyhole limpet hemocyanin (KLH)Biological: tetanus diphtheria toxoid vaccine (Td)

Interventions

Also known as: Tysabri
Tysabri Plus Vaccinations

KLH 1 mg administered subcutaneously (SC) in accordance with the Immucothel investigator's brochure.

Also known as: Immucothel
Tysabri Plus VaccinationsVaccinations Only

Td administered in accordance with the manufacturer's prescribing information.

Tysabri Plus VaccinationsVaccinations Only

Eligibility Criteria

Age18 Years - 60 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64)

You may qualify if:

  • able to give written informed consent
  • diagnosis of a relapsing form of MS and must fall within the therapeutic indication stated in the approved label for Tysabri
  • aged 18-60 years, inclusive at the time of consent
  • free of signs and symptoms suggestive of any serious opportunistic infection, based on medical history, physical examination, or laboratory testing
  • must have a known history of tetanus toxoid immunization

You may not qualify if:

  • tetanus toxoid vaccination less than 2 years prior to Screening
  • known hypersensitivity to tetanus-diphtheria vaccine or KLH or any other administered vaccinations or their components (such as thimerosal)
  • known allergy to shellfish
  • history of active tuberculosis or undergoing treatment for tuberculosis
  • previous exposure to KLH or vaccines containing KLH components (e.g., cancer vaccines)
  • known history of human immunodeficiency virus (HIV), hepatitis C, or hepatitis B infection
  • history of, or available abnormal laboratory results indicative of any significant disease
  • history of malignancy
  • history of organ transplantation (including anti-rejection therapy)
  • history of severe allergic or anaphylactic reactions or known drug hypersensitivity
  • a clinically significant infectious illness within 30 days prior to the Screening visit
  • prior exposure to Tysabri, rituximab, any murine protein, or any therapeutic monoclonal antibody at any time
  • receipt of intravenous (IV) or intramuscular (IM) immunoglobulin within 6 months of screening
  • live virus, bacterial vaccines, or any other vaccines within 3 months of screening
  • treatment with immunosuppressant medications within 6 months prior to screening
  • +6 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (10)

Research Site 1

Fullerton, California, 92835, United States

Location

Research Site

Centennial, Colorado, 80112, United States

Location

Research site

Farmington Hills, Michigan, 48334, United States

Location

Research Site

Patchogue, New York, 11772, United States

Location

Research Site 3

Charlotte, North Carolina, 28207, United States

Location

Research Site 5

Oklahoma City, Oklahoma, 73130, United States

Location

Research Site

Franklin, Tennessee, 37064, United States

Location

Research Site

Dallas, Texas, 75214, United States

Location

Research Site 2

Seattle, Washington, 98122, United States

Location

Research Site 4

Charleston, West Virginia, 25301, United States

Location

Related Publications (1)

  • Kaufman M, Pardo G, Rossman H, Sweetser MT, Forrestal F, Duda P. Natalizumab treatment shows no clinically meaningful effects on immunization responses in patients with relapsing-remitting multiple sclerosis. J Neurol Sci. 2014 Jun 15;341(1-2):22-7. doi: 10.1016/j.jns.2014.03.035. Epub 2014 Mar 26.

    PMID: 24731783BACKGROUND

MeSH Terms

Conditions

Multiple Sclerosis

Interventions

Natalizumabkeyhole-limpet hemocyanin

Condition Hierarchy (Ancestors)

Demyelinating Autoimmune Diseases, CNSAutoimmune Diseases of the Nervous SystemNervous System DiseasesDemyelinating DiseasesAutoimmune DiseasesImmune System Diseases

Intervention Hierarchy (Ancestors)

Antibodies, Monoclonal, HumanizedAntibodies, MonoclonalAntibodiesImmunoglobulinsImmunoproteinsBlood ProteinsProteinsAmino Acids, Peptides, and ProteinsSerum GlobulinsGlobulins

Results Point of Contact

Title
Biogen Idec Medical Director
Organization
Biogen Idec Inc.

Study Officials

  • Medical Director

    Biogen

    STUDY DIRECTOR

Publication Agreements

PI is Sponsor Employee
No
Restriction Type
OTHER
Restrictive Agreement
Yes

Study Design

Study Type
interventional
Phase
phase 4
Allocation
RANDOMIZED
Masking
NONE
Intervention Model
PARALLEL
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

September 25, 2007

First Posted

September 27, 2007

Study Start

January 1, 2008

Primary Completion

November 1, 2009

Study Completion

December 1, 2009

Last Updated

February 15, 2017

Results First Posted

July 29, 2011

Record last verified: 2016-12

Locations