Influence of Physical Training on Mitochondrial Function in Type 2 Diabetic Patients

NCT00365495 | Unknown

• 1 other identifier: 022
• Study Type: interventional
• Target: 30
• Locations: 1 country
• Sites: 1

Brief Summary

Physical activity as treatment for patients with type 2 diabetes (T2D). Treatment of T2D is often done by medication, changing the diet and increased physical activity. It is well established that physical training has a positive effect on the pathology of T2D such as increased insulin sensitivity and reduced fasting plasma glucose (7) and blood lipids (15). It is also well known that in healthy subjects endurance training increases mitochondrial density, lipid oxidation during submaximal exercise, and results in a number of qualitative changes in the control of OXPHOS (24). However, the effect of physical training on the mitochondrial function in skeletal muscle of patients with T2D has not been investigated. The purpose of this project is to investigate the effect of physical training on the mitochondrial respiratory function in muscles of patients with T2D. Skeletal muscle biopsies will be taken before and after 10 weeks of physical training in patients with T2D and matched control subjects. Mitochondria will be isolated from the muscle biopsies, and respiratory function, free radical production and UCP3 will be determined. Furthermore, measures of maximal oxygen consumption (VO2max), heart rate and capillary lactate concentrations and carbohydrate and lipid oxidation will be determined partly to verify an increased aerobic capacity and partly to investigate the changes in these factors in T2D patients.

Conditions

Type 2 Diabetes

Keywords

Type 2 diabetes; physical training; mitochondrial function

Outcome Measures

Primary Outcomes (1)

• Mitochondrial respiratory measurements. The investigations are carried out by two rounds - one before Christmas and one after.

Secondary Outcomes (6)

• VO2max

• Lipid oxidation

• muscle fiber type composition

• muscle oxidative enzymes

• mitochondrial-specific proteins (UCP3)

Interventions

Physical training (aerobic training) PROCEDURE

Eligibility Criteria

• Age: 40 Years - 65 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Male,
• BMI above 26,
• Age between 40 and 65,
• Living a sedentary lifestyle

You may not qualify if:

• Women
• BMI under 26
• Age under 40 and over 65
• Trained subjects Control subjects: relatives to type 2 diabetic patients, T2D trial subjects

Sponsors & Collaborators

• Odense University Hospital: lead

Study Sites (1)

University of Southern Denmark and Odense University Hospital

Odense, 5000, Denmark

Location

MeSH Terms

Conditions

Diabetes Mellitus, Type 2

Condition Hierarchy (Ancestors)

Diabetes Mellitus; Glucose Metabolism Disorders; Metabolic Diseases; Nutritional and Metabolic Diseases; Endocrine System Diseases

Study Officials

• Martin Mogensen, PhD student

  University of Southern Denmark

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Purpose: TREATMENT
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER

Study Record Dates

• First Submitted: August 15, 2006
• First Posted: August 17, 2006
• Study Start: August 1, 2006
• Study Completion: June 1, 2007
• Last Updated: April 4, 2007

Record last verified: 2007-04

Locations

DK (1)