NCT00280033

Brief Summary

This study is will gather critical information on the safety, tolerability, and the immunogenicity (capability of causing an immune response) of A/H5N1 (Bird flu) virus vaccine in healthy adults. Up to 400 healthy adults, aged 18 to 64, will participate. Each subject will participate for about 7 months and will be randomly placed in one of several different study groups receiving a different dose of vaccine or placebo. All subjects will receive two injections of their assigned study product, about 28 days apart, in the muscle. Subjects will keep a journal of their temperature and any adverse effects between study visits. A small amount of blood will also be drawn before the first injection, 7 days after each injection, 1 month after the first injection, and 1 and 6 months after the second injection.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
394

participants targeted

Target at P75+ for phase_1

Timeline
Completed

Started Feb 2006

Geographic Reach
1 country

4 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

January 19, 2006

Completed
1 day until next milestone

First Posted

Study publicly available on registry

January 20, 2006

Completed
12 days until next milestone

Study Start

First participant enrolled

February 1, 2006

Completed
9 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

November 1, 2006

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

November 1, 2006

Completed
Last Updated

September 13, 2013

Status Verified

January 1, 2009

Enrollment Period

9 months

First QC Date

January 19, 2006

Last Update Submit

September 12, 2013

Conditions

Keywords

H5N1, Influenza, vaccine, parent protocol

Outcome Measures

Primary Outcomes (5)

  • Geometric mean titer and frequency of 4-fold or greater increases in serum hemagglutination inhibition (HAI) antibody titers in each group 28 days after receipt of the second dose of vaccine.

    Approximately Day 56.

  • Adverse event or SAE information (solicited in-clinic and via memory aids, concomitant medications, and periodic targeted physical assessments).

    Adverse events will be collected through 28 days following the second dose of vaccine (approximately Day 56). Serious adverse events will be collected throughout the study through Day 208.

  • Proportion of subjects in each group achieving a serum neutralizing antibody titer ratio of 1:40 against the influenza A/H5N1 virus 28 days following second dose of vaccine.

    Approximately Day 56.

  • Proportion of subjects in each dose group achieving a serum hemagglutination (HAI) antibody titer of 1:40 against the influenza A/H5N1 virus 28 days after receipt of the second dose of vaccine.

    Approximately Day 56.

  • Geometric mean titer (GMT) and frequency of 4-fold or greater increases in neutralizing antibody titers in each group 28 days after receipt of the second dose of vaccine.

    Approximately Day 56.

Secondary Outcomes (3)

  • Geometric mean titer and the frequency of 4-fold or greater increases in neutralizing antibody titers in each group 1 month after receipt of each dose, and 7 months after receipt of the first dose of vaccine.

    Blood samples for serum assays will be collected at Day 0 and at Days 28, 56, and 208 after the first immunization.

  • Geometric mean titer and the frequency of 4-fold or greater increases in serum HAI antibody titers 1 month after receipt of each dose, and 7 months after receipt of the first dose of vaccine.

    Blood samples for serum assays will be collected at Day 0 and at Days 28, 56, and 208 after the first immunization.

  • Development of serum antibody responses against antigenically drifted variants of H5N1influenza virus.

    Blood samples for serum assays will be collected at Day 0 and at Days 28, 56, and 208 after the first immunization.

Study Arms (9)

9

PLACEBO COMPARATOR

Saline administered on days 0 and 28.

Drug: Placebo

8

EXPERIMENTAL

45 mcg alone administered on days 0 and 28.

Biological: Inactivated Influenza A/H5N1 Vaccine (Chiron)

7

EXPERIMENTAL

30 mcg plus aluminum hydroxide administered on days 0 and 28.

Biological: Aluminum hydroxideBiological: Inactivated Influenza A/H5N1 Vaccine (Chiron)

6

EXPERIMENTAL

30 mcg alone administered on days 0 and 28.

Biological: Inactivated Influenza A/H5N1 Vaccine (Chiron)

5

EXPERIMENTAL

15 mcg plus aluminum hydroxide administered on days 0 and 28.

Biological: Aluminum hydroxideBiological: Inactivated Influenza A/H5N1 Vaccine (Chiron)

4

EXPERIMENTAL

15 mcg plus MF59 administered on days 0 and 28.

Biological: Inactivated Influenza A/H5N1 Vaccine (Chiron)Biological: MF-59

3

EXPERIMENTAL

15 mcg alone administered on days 0 and 28.

Biological: Inactivated Influenza A/H5N1 Vaccine (Chiron)

2

EXPERIMENTAL

7.5 mcg plus aluminum hydroxide administered on days 0 and 28.

Biological: Aluminum hydroxideBiological: Inactivated Influenza A/H5N1 Vaccine (Chiron)

1

EXPERIMENTAL

7.5 mcg plus MF59 administered on days 0 and 28.

Biological: Inactivated Influenza A/H5N1 Vaccine (Chiron)Biological: MF-59

Interventions

Provided in vials that contain 0.8 mL volume per vial.

257

Monovalent subvirion H5N1 vaccine (HA of A/Vietnam/1203/04) provided in unit-dose vials containing 60 mcg/mL A/H5N1 HA as determined by single radial immunodiffusion. It may be formulated with MF59 or aluminum hydroxide. Dosages: 7.5 mcg, 15 mcg, 30 mcg, or 45 mcg.

12345678
MF-59BIOLOGICAL

Proprietary experimental adjuvant. Provided in vials that contain 0.7 mL volume per vial.

14

Saline placebo.

9

Eligibility Criteria

Age18 Years - 64 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Healthy male or nonpregnant female (as indicated by a negative urine pregnancy test immediately prior to vaccine administration) between the ages of 18 and 64 years, inclusive.
  • Women of childbearing potential (not surgically sterile or post menopausal for greater than or equal to one year) must agree to practice adequate contraception (i.e., barrier method, abstinence, and licensed hormonal methods) for the entire study period.
  • Is in good health, as determined by vital signs (heart rate, blood pressure, oral temperature), medical history and a targeted physical examination based on medical history.
  • Subjects should have normal safety laboratory values (Hgb, WBC, Plt, ALT, and creatinine) prior to the first immunization.
  • Able to understand and comply with planned study procedures.
  • Provides written informed consent prior to initiation of any study procedures.

You may not qualify if:

  • Has a known allergy to eggs or other components of the vaccine or latex.
  • Has a positive urine pregnancy test prior to vaccination (if female of childbearing potential) or women who are breastfeeding.
  • Is undergoing immunosuppression as a result of an underlying illness or treatment.
  • Has an active neoplastic disease or a history of any hematologic malignancy.
  • Is using oral or parenteral steroids, high-dose inhaled steroids (greater than 800 micrograms/day of beclomethasone dipropionate or equivalent) or other immunosuppressive or cytotoxic drugs (nasal and topical steroids are allowed).
  • Has a history of receiving immunoglobulin or other blood products within the 3 months prior to vaccination in this study.
  • Has received any other licensed vaccines within 2 weeks (for inactivated vaccines) or 4 weeks (for live vaccines) prior to vaccination in this study.
  • Has an acute or chronic medical condition that, in the opinion of the investigator, would render vaccination unsafe or would interfere with the evaluation of responses (this includes, but is not limited to: known chronic liver disease, significant renal disease, unstable or progressive neurological disorders, diabetes mellitus, and transplant recipients).
  • Has a history of severe reactions following immunization with contemporary influenza virus vaccines.
  • Has an acute illness, including an oral temperature greater than 100.4 degrees F, within 1 week of vaccination.
  • Received an experimental agent (vaccine, drug, biologic, device, blood product, or medication) within 1 month prior to vaccination in this study, or expects to receive an experimental agent during the 7-month study period.
  • Has any condition that would, in the opinion of the site investigator, place the subject at an unacceptable risk of injury or render the subject unable to meet the requirements of the protocol.
  • Participated in H5 vaccine study in the past.
  • Known current HIV, hepatitis B (HBsAg) or hepatitis C infection.
  • History of alcohol or drug abuse in the last 5 years.
  • +2 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (4)

Stanford University

Stanford, California, 94305-5208, United States

Location

Saint Louis University

St Louis, Missouri, 63110, United States

Location

Cincinnati Children's Hospital Medical Center

Cincinnati, Ohio, 45229-3039, United States

Location

Vanderbilt University

Nashville, Tennessee, 37232, United States

Location

Related Publications (1)

  • Bernstein DI, Edwards KM, Dekker CL, Belshe R, Talbot HK, Graham IL, Noah DL, He F, Hill H. Effects of adjuvants on the safety and immunogenicity of an avian influenza H5N1 vaccine in adults. J Infect Dis. 2008 Mar 1;197(5):667-75. doi: 10.1086/527489.

MeSH Terms

Conditions

Influenza, HumanInfluenza in Birds

Interventions

Aluminum Hydroxidechiron

Condition Hierarchy (Ancestors)

Respiratory Tract InfectionsInfectionsOrthomyxoviridae InfectionsRNA Virus InfectionsVirus DiseasesRespiratory Tract DiseasesBird DiseasesAnimal Diseases

Intervention Hierarchy (Ancestors)

HydroxidesAlkaliesInorganic ChemicalsAluminum CompoundsAnionsIonsElectrolytes

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, INVESTIGATOR
Purpose
PREVENTION
Intervention Model
PARALLEL
Sponsor Type
NIH
Responsible Party
SPONSOR

Study Record Dates

First Submitted

January 19, 2006

First Posted

January 20, 2006

Study Start

February 1, 2006

Primary Completion

November 1, 2006

Study Completion

November 1, 2006

Last Updated

September 13, 2013

Record last verified: 2009-01

Locations