NCT00140231

Brief Summary

The purpose of this study will be to determine whether giving leptin (r-metHuLeptin) to a person when he or she is fasting will reverse changes in metabolism, and hormone levels, and immune function associated with fasting, which decreases leptin levels.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
13

participants targeted

Target at below P25 for phase_1

Timeline
Completed

Started Oct 2002

Longer than P75 for phase_1

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

October 1, 2002

Completed
2.9 years until next milestone

First Submitted

Initial submission to the registry

August 30, 2005

Completed
2 days until next milestone

First Posted

Study publicly available on registry

September 1, 2005

Completed
5.5 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

March 1, 2011

Completed
5.8 years until next milestone

Study Completion

Last participant's last visit for all outcomes

December 1, 2016

Completed
6 months until next milestone

Results Posted

Study results publicly available

June 7, 2017

Completed
Last Updated

June 7, 2017

Status Verified

May 1, 2017

Enrollment Period

8.4 years

First QC Date

August 30, 2005

Results QC Date

December 29, 2015

Last Update Submit

May 5, 2017

Conditions

Keywords

leptinfastingreproductiveneuroendocrineimmune functionenergy deficiency associated with short-term fasting

Outcome Measures

Primary Outcomes (3)

  • Cortisol

    four days

  • ACTH Mean Level

    Response of ACTH to leptin administration in fed and fasting state from baseline was measured

    4 days

  • Immune Function CD3 Count

    4 days

Secondary Outcomes (3)

  • %Fat Mass

    four days

  • (RMR)

    four days

  • Autonomic Function

    four days

Study Arms (2)

Metreleptin

ACTIVE COMPARATOR

r-metHuLeptin self-administered subcutaneously

Drug: r-metHuLeptinOther: placebo

Placebo

PLACEBO COMPARATOR

Placebo, administered in same method as active arm.

Drug: r-metHuLeptinOther: placebo

Interventions

recombinant human leptin

Also known as: metreleptin
MetreleptinPlacebo
placeboOTHER

placebo (no active drug)

MetreleptinPlacebo

Eligibility Criteria

Age18 Years - 30 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Healthy lean women (with body mass indices \[BMI\] \< 25 kg/m2)
  • Overweight otherwise healthy men (with BMI \> 27 kg/m2)
  • Overweight otherwise healthy women (with BMI \> 27 kg/m2).

You may not qualify if:

  • A history of any illness that may affect the concentrations of the hormones to be studied, e.g. infectious diseases, renal or hepatic failure, type 1 or type 2 diabetes mellitus, cancer or lymphoma, hypogonadism, malabsorption or malnourishment, hypo- or hyperthyroidism, hypercortisolism, alcoholism or drug abuse, anemia, or eating disorder
  • On medications known to affect the hormones to be measured (glucocorticoids, anti-seizure medications, and thyroid hormones)
  • A known history of anaphylaxis or anaphylactoid-like reactions, or a known hypersensitivity to E. coli derived proteins

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Beth Israel Deaconess Medical Center

Boston, Massachusetts, 02215, United States

Location

Related Publications (6)

  • Chan JL, Heist K, DePaoli AM, Veldhuis JD, Mantzoros CS. The role of falling leptin levels in the neuroendocrine and metabolic adaptation to short-term starvation in healthy men. J Clin Invest. 2003 May;111(9):1409-21. doi: 10.1172/JCI17490.

    PMID: 12727933BACKGROUND
  • Bouzoni E, Perakakis N, Connelly MA, Angelidi AM, Pilitsi E, Farr O, Stefanakis K, Mantzoros CS. PCSK9 and ANGPTL3 levels correlate with hyperlipidemia in HIV-lipoatrophy, are regulated by fasting and are not affected by leptin administered in physiologic or pharmacologic doses. Metabolism. 2022 Sep;134:155265. doi: 10.1016/j.metabol.2022.155265. Epub 2022 Jul 9.

  • Chrysafi P, Perakakis N, Farr OM, Stefanakis K, Peradze N, Sala-Vila A, Mantzoros CS. Leptin alters energy intake and fat mass but not energy expenditure in lean subjects. Nat Commun. 2020 Oct 13;11(1):5145. doi: 10.1038/s41467-020-18885-9.

  • Foo JP, Aronis KN, Chamberland JP, Mantzoros CS. Lack of Day/Night variation in fibroblast growth factor 21 levels in young healthy men. Int J Obes (Lond). 2015 Jun;39(6):945-8. doi: 10.1038/ijo.2014.215. Epub 2014 Dec 26.

  • Foo JP, Aronis KN, Chamberland JP, Paruthi J, Moon HS, Mantzoros CS. Fibroblast growth factor 21 levels in young healthy females display day and night variations and are increased in response to short-term energy deprivation through a leptin-independent pathway. Diabetes Care. 2013 Apr;36(4):935-42. doi: 10.2337/dc12-0497. Epub 2012 Nov 27.

  • Moragianni VA, Aronis KN, Chamberland JP, Mantzoros CS. Short-term energy deprivation alters activin a and follistatin but not inhibin B levels of lean healthy women in a leptin-independent manner. J Clin Endocrinol Metab. 2011 Dec;96(12):3750-8. doi: 10.1210/jc.2011-1453. Epub 2011 Sep 14.

Related Links

MeSH Terms

Conditions

Fasting

Interventions

recombinant methionyl human leptinmetreleptin

Condition Hierarchy (Ancestors)

Feeding BehaviorBehavior

Limitations and Caveats

Our study is confined to lean, healthy female subjects; therefore, our results should not be generalized to male, obese,or diabetic subjects

Results Point of Contact

Title
Christos Mantzoros
Organization
BIDMC

Study Officials

  • Christos S Mantzoros, MD, DSc

    Beth Israel Deaconess Medical Center

    PRINCIPAL INVESTIGATOR

Publication Agreements

PI is Sponsor Employee
No
Restrictive Agreement
No

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
TRIPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR
Purpose
TREATMENT
Intervention Model
CROSSOVER
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Professor of Medicine

Study Record Dates

First Submitted

August 30, 2005

First Posted

September 1, 2005

Study Start

October 1, 2002

Primary Completion

March 1, 2011

Study Completion

December 1, 2016

Last Updated

June 7, 2017

Results First Posted

June 7, 2017

Record last verified: 2017-05

Locations