Study of Lung Proteins in Patients With Pneumonia

NCT00077909 | Recruiting

• 2 other identifiers: 04011904-CC-0119
• Study Type: observational
• Target: 750
• Locations: 1 country
• Sites: 1

Brief Summary

This study will examine the different types of proteins present in the lungs of patients with pneumonia to explore the causes of different types of the disease. Pneumonia is a condition that causes lung inflammation AND is often caused by an infection. It is usually diagnosed by lung x-rays and listening to the chest with a stethoscope. This method can diagnose pneumonia, but it does not provide information on the cause of the inflammation - information that might be helpful in guiding treatment. This study will measure proteins in the lungs of patients to see if certain proteins are associated with specific forms of pneumonia, and can thus serve as biomarkers for disease. Patients undergoing diagnostic bronchoscopy at the NIH Clinical Center may participate in this study. Patients will undergo bronchoscopy and bronchoalveolar lavage as scheduled for their medical care. For this procedure, the patient's mouth and throat are numbed with lidocaine; a sedative may be given for comfort. A thin flexible tube called a bronchoscope is advanced through the nose or mouth into the lung airways to examine the airways carefully. Saline (salt water) is then injected through the bronchoscope into the air passage, acting as a rinse. A sample of fluid is then withdrawn for microscopic examination. Researchers in the current study will use some of the fluid obtained from the lavage to examine for protein content. In addition to the bronchoscopy and bronchoalveolar lavage, participants will have about 2 tablespoons of blood drawn to compare blood test results with the results of the lung washings. Patients' medical records will be reviewed to obtain information on past medical history, current medical treatment, vital signs, and results of x-ray tests. ...

Conditions

Pneumonia; Pulmonary Disease; Lung Disease

Keywords

Proteomics; Infection; Pneumonia; Mass Spectrometry; BAL; Natural History; Lung; Pulmonary Disease; Lung Infiltrates

Outcome Measures

Primary Outcomes (2)

• Peptide biomarker

  Detection of peptide biomarker for infectious disease as a cause of pneumonia.

  Batch-driven analysis of samples.

• Characterization of lung cells associated with pneumonia

  Analyze lung cells associated with infectious or inflammatory pulmonary conditions.

  Batch-driven analysis of samples.

Study Arms (2)

Group 1

Patients with Infectious Pneumonia

Group 2

Patients with Non-Infectious Pneumonia

Eligibility Criteria

• Age: 3 Years - 99 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Any patient at the NIH Clinical Center who has a clinically indicated need for diagnostic bronchoscopy.@@@

You may qualify if:

• All eligible patients undergoing diagnostic bronchoscopy who provide consent for proteomic analysis of BAL fluid supernatant and chart review of patient characteristics will be included in this study.
• A parent/guardian may provide consent for a child age 17 or under and a Legally Authorized Representative (LAR) may provide consent for adults unable to consent.

You may not qualify if:

• Patients undergoing bronchoscopy but not wanting to participate with either the chart review or the proteomic analysis of BAL fluid supernatant will be excluded.

Sponsors & Collaborators

• National Institutes of Health Clinical Center (CC): lead

Study Sites (1)

National Institutes of Health Clinical Center

Bethesda, Maryland, 20892, United States

RECRUITING

Related Publications (3)

• Wang H, Drake SK, Yong C, Gucek M, Lyes MA, Rosenberg AZ, Soderblom E, Arthur Moseley M, Dekker JP, Suffredini AF. A Genoproteomic Approach to Detect Peptide Markers of Bacterial Respiratory Pathogens. Clin Chem. 2017 Aug;63(8):1398-1408. doi: 10.1373/clinchem.2016.269647. Epub 2017 Jun 6.

  PMID: 28588123 BACKGROUND

• Wang H, Drake SK, Yong C, Gucek M, Tropea M, Rosenberg AZ, Dekker JP, Suffredini AF. A Novel Peptidomic Approach to Strain Typing of Clinical Acinetobacter baumannii Isolates Using Mass Spectrometry. Clin Chem. 2016 Jun;62(6):866-75. doi: 10.1373/clinchem.2015.253468. Epub 2016 Apr 26.

  PMID: 27117471 BACKGROUND

• Wang H, Drake SK, Youn JH, Rosenberg AZ, Chen Y, Gucek M, Suffredini AF, Dekker JP. Peptide Markers for Rapid Detection of KPC Carbapenemase by LC-MS/MS. Sci Rep. 2017 May 31;7(1):2531. doi: 10.1038/s41598-017-02749-2.

  PMID: 28566732 BACKGROUND

Related Links

• NIH Clinical Center Detailed Web Page

MeSH Terms

Conditions

Pneumonia; Lung Diseases; Infections

Condition Hierarchy (Ancestors)

Respiratory Tract Infections; Respiratory Tract Diseases

Study Officials

• Anthony F Suffredini, M.D.

  National Institutes of Health Clinical Center (CC)

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Gloria Y Pastor, R.N.

CONTACT

(301) 402-3484; gloria.pastor@nih.gov

Anthony F Suffredini, M.D.

CONTACT

(301) 496-9320; asuffredini@mail.nih.gov

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: NIH
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: February 12, 2004
• First Posted: February 13, 2004
• Study Start: February 20, 2004
• Last Updated: June 8, 2026

Record last verified: 2026-04-09

Locations

US (1)