Effects of a Physical Exercise Training Program on Hormonal and Mineral Biomarkers and Aerobic Fitness in Male University Students: A Randomized Controlled Trial
Sponsor study identifier
interventional
29
1 country
1
Study Start
December 1, 2025
Actual (reported)Primary Completion
February 18, 2026
Actual (reported)Study Completion
February 18, 2026
Actual (reported)The purpose of this study is to investigate the effects of a high-intensity interval training (HIIT) program on hormonal and mineral biomarkers and aerobic fitness in male university students. The study aims to determine whether HIIT improves hormonal biomarkers, including testosterone, cortisol, the testosterone-to-cortisol ratio, and growth hormone (GH), as well as mineral metabolism biomarkers, including parathyroid hormone (PTH), 25-hydroxyvitamin D \[25(OH)D\], calcium, and phosphate. Participants will be assigned to either a HIIT group or a non-training control group. The HIIT group completed three sessions per week for 9 weeks, alternating 30-second runs at high-intensity and low-intensity. The control group did not participate in the training intervention and maintained its usual daily activities.
Registry-reported information. Elapsed time and enrollment totals alone do not measure study progress or recruitment pace.
February 18, 2026
Actual (reported)Click on a node to explore related trials.
Dates and qualifiers reported by the registry. Estimated dates do not confirm that a milestone has occurred.
December 1, 2025
Actual (reported)February 18, 2026
Actual (reported)February 18, 2026
Actual (reported)October 19, 2026
Actual (reported)October 26, 2026
Actual (reported)Testosterone
Testosterone concentration, expressed in µg/dL, was measured using enzyme-linked immunosorbent assay (ELISA) kits (Monobind, Inc., Lake Forest, CA).
One day before the start and two days after the completion of the 09 weeks of training program
Cortisol
Cortisol concentration, expressed in µg/dL, was measured using an automated chemiluminescence immunoassay (DiaSorin Inc., Stillwater, MN).
One day before the start and two days after the completion of the 09 weeks of training program.
Testosterone/cortisol ratio
Testosterone/cortisol ratio (T/C) was calculated by dividing the testosterone value by the cortisol value for each participant.
One day before the start and two days after the completion of the 09 weeks of training program.
Participants in the intervention group completed a high-intensity interval training (HIIT) program consisting of three sessions per week for 9 weeks. Each session comprised three sets of 6-8 repetitions of 30-second running bouts performed at 100-110% of maximal aerobic speed (MAS), interspersed with 30-second active recovery runs at 50% of MAS.
Participants in the control group maintained their usual daily activities and did not participate in the HIIT intervention throughout the 9-week study period.
The high-intensity interval training program consisted of three sessions per week for 9 weeks. Each session comprised three sets of 6-8 repetitions of 30-second running bouts performed at 100-110% of maximal aerobic speed (MAS), interspersed with 30-second active recovery runs at 50% of MAS between bouts.
High Institute of Sport and Physical Education of Kef, University of Jendouba Kef, Boulifa, Tunisia, 7100
El Kef, Boulifa, 7100, Tunisia
Nejmeddine Ouerghi, Ph.D
Research Unit "Sport Sciences, Health and Movement"(UR22JS01) High Institute of Sport and Physical Education of Kef, University of Jendouba, 7100 Kef, Tunisia.
29 participants (actual enrollment reported by the registry).
First Submitted
October 19, 2026
First Posted
October 26, 2026
Study Start
December 1, 2025
Actual (reported)Parathyroid Hormone
Parathyroid hormone concentration, expressed in pg/mL, was measured using an automated chemiluminescence immunoassay (DiaSorin Inc., Stillwater, MN).
One day before the start and two days after the completion of the 09 weeks of training program.
Growth hormone
Growth hormone concentration, expressed in mIU/L, was measured using an immunoradiometric assay (IRMA GH; Beckman Coulter).
One day before the start and two days after the completion of the 09 weeks of training program.
25-hydroxyvitamin D
25-hydroxyvitamin D \[25(OH)D\] concentration, expressed in ng/mL, was analyzed using an automated chemiluminescence immunoassay (DiaSorin Inc., Stillwater, MN).
One day before the start and two days after the completion of the 09 weeks of training program.
Aerobic capacity
Aerobic capacity was assessed using the Vameval incremental running test performed to exhaustion (Cazorla, 1999). Maximal aerobic speed (MAS), expressed in km/h, was determined from the highest running speed achieved during the test, and maximal oxygen uptake (VO₂max), expressed in mL·kg-¹·min-¹, was estimated using the Vameval protocol (Cazorla, 1999).
One day before the start and two days after the completion of the 09 weeks of training program.
Calcium
One day before the start and two days after the completion of the 09 weeks of training program.
Phosphorus
One day before the start and two days after the completion of the 09 weeks of training program.
Contact the study team to confirm eligibility.
Primary Completion
February 18, 2026
Actual (reported)Study Completion
February 18, 2026
Actual (reported)Last Updated
October 26, 2026
Record last verified: 2026-10
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