Evaluation of Postoperative Pain and the Antimicrobial Effect of Super Oxidized Water (Microcyn) and Chlorhexidine When Compared With Sodium Hypochlorite in Teeth With Asymptomatic Necrotic Pulps. A Randomized Clinical Trial

NCT07852260 | Not Yet Recruiting DMC

• 1 other identifier: Paunceh samy
• Study Type: interventional
• Target: 75
• Locations: 1 country
• Sites: 1

Brief Summary

The objective of this study is to evaluate the postoperative pain and antimicrobial efficacy when using super-oxidized water (Microcyn) and 2% Chlorhexidine as irrigating solutions in comparison to 2.5% Sodium hypochlorite in teeth with asymptomatic necrotic pulps.

Conditions

Evaluation of Postoperative Pain and Antimicrobial Efficacy

Keywords

Postoperative pain, NRS, Bacterial load reduction

Outcome Measures

Primary Outcomes (1)

• Postoperative pain intensity at 6 hours post-instrumentation

  A pain chart using numerical rating scale (NRS) will then be used to record the patients' pain level. The NRS (0-10 scale) consists of a line anchored by two extremes "No pain" and "the worst pain". Patients will be asked to choose the mark that represented their level of pain from 0 to 10. Pain level will be assigned as follow: 0: no pain, 1-3: mild pain, 4-6: moderate pain, 7-10: severe pain.

  6 hours post-instrumentation

Study Arms (3)

Group A

ACTIVE COMPARATOR

Each canal will receive 2ml of super-oxidized water (Microcyn) irrigating solution between each instrument size and the subsequent one.

Other: super-oxidized water (Microcyn) irrigating solution

Group B

ACTIVE COMPARATOR

Each canal will receive 2ml of 2% chlorhexidine irrigating solution between each instrument size and the subsequent one.

Other: 2% chlorhexidine irrigating solution

Group C

ACTIVE COMPARATOR

Each canal will receive 2ml of 2.5 % sodium hypochlorite irrigating solution between each instrument size and the subsequent one.

Other: 2.5% sodium hypochlorite

Interventions

super-oxidized water (Microcyn) irrigating solution OTHER

The difference in concentration of chlorine between NaOCl and super oxidized water such that the concentration of chlorine ions in NaOCl is much higher than that in super-oxidized water. However, considering the 90% reduction in biofilm thickness and 98% reduction in colony count of microorganisms by super-oxidized water as demonstrated

Group A

2% chlorhexidine irrigating solution OTHER

Chlorhexidine (CHX) has been indicated as an alternative irrigation agent in the preparation of the root canal system, due to its broad-spectrum antimicrobial capacity, dentin tubule disinfection, dentin adsorption, high substantivity, and low toxicity compared to NaOCl. The inability to dissolve organic tissues of the CHX provides controversial in the use of this irrigant, and due to this limitation, some authors do not indicate it as the main irrigation solution in endodontic treatment despite its utility as a final irrigation solution.

Group B

2.5% sodium hypochlorite OTHER

Sodium hypochlorite (NaOCl) is the most common and has been widely used in endodontics for more than 70 years because of its ability to act as a solvent for organic matter and its efficient antimicrobial action, however, at high concentrations, it is likely to irritate periradicular tissues.

Group C

Eligibility Criteria

• Age: 18 Years - 50 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64)

You may qualify if:

• Patients in good physical health; American Society of Anesthesiologists (ASA) class I or II.
• Patients whose age ranges between 18 and 50 years.
• Male and female patients.
• Patients having a single canal-tooth with asymptomatic necrotic pulp.
• Patients who can understand the number rating scale.
• Patients who accept to participate in the trial and can sign the informed consent

You may not qualify if:

• Pregnant females.
• Patients with intraoral or extraoral swelling.
• Patients whose tooth is non-restorable.
• Teeth with open apices.
• Teeth with radiographic evidence of root resorption.

Sponsors & Collaborators

• Cairo University: lead

Study Sites (1)

faculty of Dentistry Cairo university

Cairo, Cairo Governorate, 11553, Egypt

Location

MeSH Terms

Conditions

Pain, Postoperative

Interventions

Sodium Hypochlorite

Condition Hierarchy (Ancestors)

Postoperative Complications; Pathologic Processes; Pathological Conditions, Signs and Symptoms; Pain; Neurologic Manifestations; Signs and Symptoms

Intervention Hierarchy (Ancestors)

Hypochlorous Acid; Chlorine Compounds; Inorganic Chemicals; Oxides; Oxygen Compounds; Sodium Compounds

Central Study Contacts

Paunceh S Soliman, PHD candidate

CONTACT

01026999750; paunceh.soliman@dentistry.cu.edu.eg

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: QUADRUPLE
• Who Masked: PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
• Masking Details: -In this proposed study, both the participant and assessor will be blinded. This is achieved where the outcome assessor will not be informed of the group in which the participant is enrolled for subjective outcomes. The participants will be blinded to the study hypothesis as to which intervention is expected to be better. * The patients who already do not know their treatment group will assess the level of their postoperative pain. * The laboratory technician at the microbiological department will not know the treatment group of the patients. * The treatment groups will remain anonymous at the end of the study during assessment by the statistician.
• Purpose: TREATMENT
• Intervention Model: FACTORIAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: PHD candidate

Model Details: Group A: Each canal will receive 2ml of super-oxidized water (Microcyn) irrigating solution between each instrument size and the subsequent one. Group B: Each canal will receive 2ml of 2% chlorhexidine irrigating solution between each instrument size and the subsequent one. Group C: Each canal will receive 2ml of 2.5 % sodium hypochlorite irrigating solution between each instrument size and the subsequent one.

Study Record Dates

• First Submitted: September 25, 2026
• First Posted: October 1, 2026
• Study Start (Estimated): November 2, 2026
• Primary Completion (Estimated): November 2, 2027
• Study Completion (Estimated): November 2, 2027
• Last Updated: October 1, 2026

Record last verified: 2026-09

Data Sharing

• IPD Sharing: Will not share

Locations

EG (1)