A-PRF AND HA AFTER IMPACTED THIRD MOLAR SURGERY

NCT07835373 | Completed

• 1 other identifier: 2025/8124
• Study Type: interventional
• Target: 47
• Locations: 1 country
• Sites: 1

Brief Summary

This randomized controlled clinical trial evaluated the effects of advanced platelet-rich fibrin (A-PRF) and hyaluronic acid (HA) on healing after surgical removal of impacted mandibular third molars. Participants were assigned to one of three groups: A-PRF applied to the extraction socket, HA applied to the extraction socket, or a control group receiving no additional biomaterial. Postoperative pain, facial swelling, and maximum mouth opening were assessed during the first postoperative week. Radiographic bone healing was evaluated using fractal dimension and mean gray value measurements on panoramic radiographs obtained on postoperative days 7 and 90. The study aimed to determine whether A-PRF or HA could improve postoperative clinical recovery and radiographic bone healing compared with standard treatment alone.

Conditions

Third Molar Surgery

Keywords

Hyaluronic acid; impacted third molar; fractal analysis; Radiographic gray value; advanced platelet-rich fibrin

Outcome Measures

Primary Outcomes (1)

• Fractal Dimension of the Extraction Socket

  Fractal dimension (FD) was used to evaluate the trabecular bone pattern within the extraction socket on digital panoramic radiographs. A standardized 32 × 32-pixel region of interest was selected at the center of the extraction socket, and FD was calculated using the box-counting method in ImageJ.

  Postoperative days 7 and 90

Secondary Outcomes (4)

• Mean Radiographic Gray Value

  Postoperative days 7 and 90

• Postoperative Pain

  Postoperative days 1, 3, and 7

• Postoperative Facial Swelling

  Baseline and postoperative days 1, 3, and 7

• Maximum Mouth Opening

  Baseline and postoperative days 1, 3, and 7

Study Arms (3)

A-PRF Group

EXPERIMENTAL

Advanced platelet-rich fibrin (A-PRF) was applied to the extraction socket following surgical removal of the impacted mandibular third molar.

Biological: Advanced Platelet-Rich Fibrin

Hyaluronic Acid Group

EXPERIMENTAL

One milliliter of 0.8% sodium hyaluronate gel was applied to the extraction socket following surgical removal of the impacted mandibular third molar.

Drug: Sodium hyaluronate

Control Group

NO INTERVENTION

No additional biomaterial was applied to the extraction socket following surgical removal of the impacted mandibular third molar. Participants received the same surgical procedure and standard postoperative care as the other groups.

Interventions

Sodium hyaluronate DRUG

One milliliter of 0.8% sodium hyaluronate gel was applied directly into the extraction socket immediately after surgical removal of the impacted mandibular third molar.

Hyaluronic Acid Group

Advanced Platelet-Rich Fibrin BIOLOGICAL

Advanced platelet-rich fibrin (A-PRF) was prepared from autologous venous blood by centrifugation at 1500 rpm for 14 minutes. The resulting A-PRF membrane was placed into the extraction socket immediately after surgical removal of the impacted mandibular third molar.

A-PRF Group

Eligibility Criteria

• Age: 19 Years - 29 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Patients aged 19-29 years. American Society of Anesthesiologists (ASA) physical status I. Presence of an impacted mandibular third molar indicated for surgical removal. Ability and willingness to comply with the study procedures and follow-up visits.
• Provision of written informed consent.

You may not qualify if:

• Smoking. Poor oral hygiene. Presence of acute infection in the surgical area. Presence of a cystic or tumoral lesion associated with the impacted tooth. Uncontrolled periodontal disease. Refusal to participate or withdrawal of consent. Use of analgesics or antibiotics within 12 hours before surgery. Surgical procedure lasting longer than 40 minutes.

Sponsors & Collaborators

• Inonu University: lead

Study Sites (1)

Inonu University Faculty of Dentistry

Malatya, Battalgazi, 44200, Turkey (Türkiye)

Location

MeSH Terms

Interventions

Hyaluronic Acid

Intervention Hierarchy (Ancestors)

Glycosaminoglycans; Polysaccharides; Carbohydrates

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: OUTCOMES ASSESSOR
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Research Assistant

Study Record Dates

• First Submitted: September 17, 2026
• First Posted: September 22, 2026
• Study Start: August 11, 2025
• Primary Completion: June 30, 2026
• Study Completion: June 30, 2026
• Last Updated: September 29, 2026

Record last verified: 2026-09

Data Sharing

• IPD Sharing: Will share
• Shared Documents: STUDY PROTOCOL, SAP, CSR
• Time Frame: The study will be prepared for publication as a manuscript within six months.
• Access Criteria: All individual participant data (IPD) and supporting information will be available after publication of the article.

Locations

TU (1)