Effects of Magnesium and Spinning Training on Echocardiographic, Inflammatory, and Aerobic Outcomes

NCT07662954 | Enrolling By Invitation

• 1 other identifier: 02-2026/29
• Study Type: interventional
• Target: 48
• Locations: 1 country
• Sites: 1

Brief Summary

Regular aerobic exercise may improve cardiorespiratory fitness while modulating systemic inflammation and oxidative stress. Spinning, as a structured indoor cycling modality, provides a practical aerobic exercise model with adjustable intensity and duration, but its effects on redox balance, inflammatory status, aerobic capacity, and cardiac functional parameters may vary according to individual recovery and nutritional status. Magnesium is an essential micronutrient involved in energy metabolism, muscle contraction-relaxation, ion regulation, and inflammatory and oxidative pathways; therefore, magnesium use may be relevant to exercise adaptation and recovery. This study aims to evaluate the associations of magnesium use and spinning training with oxidative stress, inflammation, aerobic capacity, and cardiac parameters. In this context, biochemical markers related to oxidative/antioxidant status and inflammation, aerobic performance indicators, and echocardiographic cardiac function parameters will be assessed together. The study is expected to provide real-world evidence on whether magnesium use in individuals participating in spinning training is associated with more favorable redox, inflammatory, aerobic, and cardiac profiles.

Conditions

Oxidative Stress; Inflammation; Physical Fitness; Echocardiography; Exercise; Healthy Volunteers

Outcome Measures

Primary Outcomes (2)

• Change in Maximal Oxygen Uptake (VO₂max)

  Maximal oxygen uptake (VO₂max, mL/kg/min) will be assessed using a graded exercise test. The change in VO₂max from baseline to 6 weeks will be evaluated.

  Baseline and 6 weeks

• Change in Serum Magnesium Concentration

  Serum magnesium concentration (mg/dL) will be measured using standard laboratory methods. The change in serum magnesium levels from baseline to 6 weeks will be evaluated.

  Baseline and 6 weeks

Secondary Outcomes (11)

• Change in Total Antioxidant Status (TAS)

  Baseline and 6 weeks

• Change in Total Oxidant Status (TOS)

  Baseline and 6 weeks

• Change in Paraoxonase-1 Activity (PON1)

  Baseline and 6 weeks

• Change in Arylesterase Activity (ARE)

  Baseline and 6 weeks

• Change in High-Sensitivity C-Reactive Protein (hs-CRP)

  Baseline and 6 weeks

Study Arms (3)

Control group

NO INTERVENTION

Participants in this arm will not receive any exercise training, magnesium supplementation, or placebo intervention during the study period. They will continue their usual daily routines and will undergo the same baseline and follow-up assessments as the intervention groups.

Spinning Training Group

ACTIVE COMPARATOR

Participants in this arm will participate in a structured spinning training program during the study period. No magnesium supplementation or placebo will be administered. Baseline and follow-up assessments will include oxidative stress, inflammatory, aerobic capacity, and cardiac function parameters.

Behavioral: Spinning Training

Spinning + Magnesium Group

EXPERIMENTAL

Participants in this arm will participate in a structured spinning training program and receive magnesium supplementation during the study period. Baseline and follow-up assessments will include oxidative stress, inflammatory, aerobic capacity, and cardiac function parameters.

Behavioral: Spinning Training; Dietary Supplement: Magnesium Supplementation

Interventions

Spinning Training BEHAVIORAL

Participants will participate in a structured spinning training program during the study period. The program will consist of supervised indoor cycling sessions performed at a planned frequency, duration, and intensity.

Spinning + Magnesium Group; Spinning Training Group

Magnesium Supplementation DIETARY_SUPPLEMENT

Participants will receive oral magnesium supplementation during the study period in addition to the structured spinning training program.

Spinning + Magnesium Group

Eligibility Criteria

• Age: 18 Years - 30 Years
• Gender Eligibility Details: Self-identified male participants are eligible
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Self-identified male participants
• Aged 18 to 30 years
• Physically active individuals
• Participants who are eligible to participate in a structured spinning training program
• Participants with available baseline and follow-up assessments for biochemical, aerobic capacity, and cardiac function parameters

You may not qualify if:

• Presence of any chronic systemic disease
• Acute infection during the study period
• Regular use of anti-inflammatory or antioxidant medications
• Use of antioxidant or ergogenic supplements other than magnesium
• Any cardiovascular, musculoskeletal, or neurological condition that may limit participation in spinning training
• Missing baseline or follow-up outcome data

Sponsors & Collaborators

• Karamanoğlu Mehmetbey University: lead

Study Sites (1)

Karamanoğlu Mehmetbey University, Faculty of Sport Sciences

Karaman, 70100, Turkey (Türkiye)

Location

Related Publications (7)

• Tarsitano MG, Quinzi F, Folino K, Greco F, Oranges FP, Cerulli C, Emerenziani GP. Effects of magnesium supplementation on muscle soreness in different type of physical activities: a systematic review. J Transl Med. 2024 Jul 5;22(1):629. doi: 10.1186/s12967-024-05434-x.

  PMID: 38970118 BACKGROUND

• Sireno L, Dimauro I, Caporossi D. Reactive oxygen species in exercise biology: from adaptive stress response to cell signaling and beyond. Free Radic Biol Med. 2026 Mar 1;245:447-462. doi: 10.1016/j.freeradbiomed.2025.12.044. Epub 2026 Jan 2. No abstract available.

  PMID: 41485544 BACKGROUND

• Reno AM, Green M, Killen LG, O'Neal EK, Pritchett K, Hanson Z. Effects of Magnesium Supplementation on Muscle Soreness and Performance. J Strength Cond Res. 2022 Aug 1;36(8):2198-2203. doi: 10.1519/JSC.0000000000003827. Epub 2020 Oct 1.

  PMID: 33009349 BACKGROUND

• Feng W, Wang Y, Gu X, Yu D, Liu Z. Exercise as a modulator of systemic inflammation and oxidative stress biomarkers across clinical and healthy populations: an umbrella meta-analysis. BMC Sports Sci Med Rehabil. 2025 Nov 28;17(1):360. doi: 10.1186/s13102-025-01327-8.

  PMID: 41316311 BACKGROUND

• Dominguez LJ, Veronese N, Ragusa FS, Baio SM, Sgro F, Russo A, Battaglia G, Bianco A, Barbagallo M. The Importance of Vitamin D and Magnesium in Athletes. Nutrients. 2025 May 13;17(10):1655. doi: 10.3390/nu17101655.

  PMID: 40431395 BACKGROUND

• Chavarrias M, Carlos-Vivas J, Collado-Mateo D, Perez-Gomez J. Health Benefits of Indoor Cycling: A Systematic Review. Medicina (Kaunas). 2019 Aug 8;55(8):452. doi: 10.3390/medicina55080452.

  PMID: 31443139 BACKGROUND

• Battista RA, Foster C, Andrew J, Wright G, Lucia A, Porcari JP. Physiologic responses during indoor cycling. J Strength Cond Res. 2008 Jul;22(4):1236-41. doi: 10.1519/JSC.0b013e318173dbc4.

  PMID: 18545183 BACKGROUND

MeSH Terms

Conditions

Inflammation; Motor Activity

Condition Hierarchy (Ancestors)

Pathologic Processes; Pathological Conditions, Signs and Symptoms; Behavior

Study Officials

• Şemsi Gül Yılmaz Kocaman, PhD

  Karamanoğlu Mehmetbey University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: OUTCOMES ASSESSOR
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: June 5, 2026
• First Posted: June 23, 2026
• Study Start: June 1, 2026
• Primary Completion (Estimated): August 15, 2026
• Study Completion (Estimated): August 20, 2026
• Last Updated: June 23, 2026

Record last verified: 2026-06

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)