NCT07653997

Brief Summary

This project is a randomized controlled clinical study investigating the effect of Mineral Trioxide Aggregate (MTA) used as an apical plug in root canal obturation on periodontal healing and inflammatory biomarkers in teeth with combined endodontic-periodontal (endo-perio) lesions. The study compares MTA with resin-based sealers and evaluates clinical, radiographic, and biochemical outcomes, including levels of IL-10, TNF-α, and MMP-8 in gingival crevicular fluid. The ultimate goal is to determine which treatment approach improves healing and increases tooth survival in such complex lesions.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
120

participants targeted

Target at P50-P75 for not_applicable

Timeline
Completed

Started Mar 2024

Typical duration for not_applicable

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

March 20, 2024

Completed
1.1 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

April 10, 2025

Completed
1 year until next milestone

Study Completion

Last participant's last visit for all outcomes

April 20, 2026

Completed
16 days until next milestone

First Submitted

Initial submission to the registry

May 6, 2026

Completed
1 month until next milestone

First Posted

Study publicly available on registry

June 17, 2026

Completed
Last Updated

June 17, 2026

Status Verified

May 1, 2026

Enrollment Period

1.1 years

First QC Date

May 6, 2026

Last Update Submit

June 13, 2026

Conditions

Keywords

Endo-perio lesionRoot canal treatmentMTAPeriodontal healingGingival crevicular fluid

Outcome Measures

Primary Outcomes (3)

  • Change in Periapical Index (PAI) Score

    Periapical healing will be evaluated using the Periapical Index (PAI) score on periapical radiographs obtained at baseline and 1 year after treatment.

    Baseline and 1 year

  • Change in Probing Depth (PD)

    Periodontal healing will be assessed by measuring probing depth (PD) in millimeters at baseline and 1 year after treatment.

    Baseline and 1 year

  • Change in Clinical Attachment Level (CAL)

    Periodontal healing will be assessed by measuring clinical attachment level (CAL) in millimeters at baseline and 1 year after treatment.

    Baseline and 1 year

Secondary Outcomes (3)

  • Change in TNF-α Concentration in Gingival Crevicular Fluid

    Baseline and 1 year

  • Change in IL-10 Concentration in Gingival Crevicular Fluid

    Baseline and 1 year

  • Change in MMP-8 Concentration in Gingival Crevicular Fluid

    Baseline and 1 year

Study Arms (4)

Endo-Perio + MTA

EXPERIMENTAL

Teeth with combined endodontic-periodontal lesions treated with MTA apical plug and root canal therapy.

Procedure: Root Canal Treatment with MTA Apical PlugProcedure: Periodontal Therapy (Scaling and Root Planing)Other: Gingival Crevicular Fluid Sampling

Endo-Perio + Resin Sealer

ACTIVE COMPARATOR

Teeth with combined endo-perio lesions treated with resin-based root canal sealer (AH Plus).

Procedure: Root Canal Treatment with Resin-Based Sealer (AH Plus)Procedure: Periodontal Therapy (Scaling and Root Planing)Other: Gingival Crevicular Fluid Sampling

AP + MTA

EXPERIMENTAL

Teeth with apical periodontitis treated with MTA apical plug.

Procedure: Root Canal Treatment with MTA Apical PlugOther: Gingival Crevicular Fluid Sampling

AP + Resin Sealer

ACTIVE COMPARATOR

Teeth with apical periodontitis treated with resin-based sealer (AH Plus).

Procedure: Root Canal Treatment with Resin-Based Sealer (AH Plus)Other: Gingival Crevicular Fluid Sampling

Interventions

Standardized root canal treatment performed under aseptic conditions. Following chemomechanical preparation, an apical plug is created using Mineral Trioxide Aggregate (MTA), and the remaining canal is obturated with resin-based sealer and gutta-percha.

AP + MTAEndo-Perio + MTA

Standardized root canal treatment performed under aseptic conditions. After chemomechanical preparation, the canal is obturated using gutta-percha in combination with an epoxy resin-based sealer (AH Plus).

AP + Resin SealerEndo-Perio + Resin Sealer

Non-surgical periodontal treatment including scaling and root planing performed following endodontic treatment in patients diagnosed with combined endodontic-periodontal lesions.

Endo-Perio + MTAEndo-Perio + Resin Sealer

Collection of gingival crevicular fluid samples at baseline and follow-up visits for the analysis of inflammatory biomarkers (IL-10, TNF-α, MMP-8) using ELISA.

AP + MTAAP + Resin SealerEndo-Perio + MTAEndo-Perio + Resin Sealer

Eligibility Criteria

Age18 Years - 60 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Aged between 18 and 60 years Systemically healthy individuals Presence of radiographically confirmed periapical lesion Diagnosis of apical periodontitis and/or combined endodontic-periodontal lesion Ability to understand and sign informed consent Willingness to attend follow-up visits

You may not qualify if:

  • Patients with severe systemic diseases (ASA III-VI) Teeth with advanced mobility (Miller Class III or higher) Patients unable to tolerate dental treatment procedures Patients unwilling to participate or attend follow-up visits

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Istanbul Medipol University, Faculty of Dentistry, Department of Endodontics

Istanbul, Turkey (Türkiye)

Location

MeSH Terms

Conditions

Periapical PeriodontitisPeriapical Diseases

Interventions

Tooth ExfoliationRoot Planing

Condition Hierarchy (Ancestors)

Jaw DiseasesStomatognathic DiseasesPeriodontal DiseasesMouth DiseasesPeriodontitis

Intervention Hierarchy (Ancestors)

Dental Physiological PhenomenaDigestive System and Oral Physiological PhenomenaDental ScalingDental ProphylaxisPeriodonticsDentistrySubgingival CurettagePreventive Dentistry

Study Officials

  • yelda erdem hepsenoglu, Assoc.Prof.

    Istanbul Medipol University, Faculty of Dentistry, Department of Endodontics

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
SINGLE
Who Masked
OUTCOMES ASSESSOR
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Assoc. Prof. Yelda Erdem Hepşenoğlu

Study Record Dates

First Submitted

May 6, 2026

First Posted

June 17, 2026

Study Start

March 20, 2024

Primary Completion

April 10, 2025

Study Completion

April 20, 2026

Last Updated

June 17, 2026

Record last verified: 2026-05

Data Sharing

IPD Sharing
Will share

De-identified individual participant data underlying the published results will be made available upon reasonable request following publication of the primary study results.

Shared Documents
STUDY PROTOCOL
Time Frame
Beginning after publication of the primary study results.
Access Criteria
Access will be provided to qualified researchers upon reasonable request and approval by the study investigators.

Locations