NCT07652216

Brief Summary

This prospective clinical study evaluates bone healing following dentigerous cyst enucleation associated with impacted mandibular third molar extraction. The study compares spontaneous blood clot healing, advanced platelet-rich fibrin (A-PRF), and A-PRF combined with allogenic dentin-derived matrix (ADDM). Bone healing is assessed as the primary outcome, while postoperative pain intensity assessed using the Numerical Rating Scale (NRS) with graphical representation represents a secondary outcome. The study aims to determine whether the use of A-PRF or A-PRF combined with ADDM improves postoperative healing outcomes compared with spontaneous blood clot healing.

Trial Health

75
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
35

participants targeted

Target at P25-P50 for not_applicable

Timeline
9mo left

Started Apr 2024

Typical duration for not_applicable

Geographic Reach
1 country

1 active site

Status
active not recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Progress74%
Apr 2024Apr 2027

Study Start

First participant enrolled

April 4, 2024

Completed
2.2 years until next milestone

First Submitted

Initial submission to the registry

June 11, 2026

Completed
6 days until next milestone

First Posted

Study publicly available on registry

June 17, 2026

Completed
6 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 1, 2026

Expected
4 months until next milestone

Study Completion

Last participant's last visit for all outcomes

April 1, 2027

Last Updated

June 17, 2026

Status Verified

June 1, 2026

Enrollment Period

2.7 years

First QC Date

June 11, 2026

Last Update Submit

June 11, 2026

Conditions

Keywords

Autogenous demineralized dentin matrixdentin matrixdentigerous cystmandible cystImpacted molarprfa-prfplatelet rich fibrinimpacted lower third molarbone regenerationdemineralized dentin matrix

Outcome Measures

Primary Outcomes (1)

  • Bone regeneration

    Cone- beam computed tomography (CBCT) assessment of bone healing following enucleation of mandibular dentigerous cysts associated with impacted mandibular third molars treated with different regenerative approaches.

    6 and 12 months postoperatively

Secondary Outcomes (1)

  • Postoperative Pain Intensity

    Day of surgery, posteoperative day 2 and 6.

Study Arms (3)

Blood Clot Healing

ACTIVE COMPARATOR

Healing of the postoperative defect following enucleation of mandibular dentigerous cyst associated with extraction of impacted mandibular third molar without additional regenerative biomaterials.

Procedure: Impacted third molar extraction with associated dentigerous cyst enucleation- blood clot healing

A-PRF

EXPERIMENTAL

Application of advanced platelet rich fibrin (A-PRF) into the postoperative defect following enucleation of mandibular dentigerous cyst associated with extraction of impacted mandibular third molar.

Procedure: Impacted third molar extraction with associated dentigerous cyst enucleation- regeneration with Advanced Platelet Rich Fibrin

A-PRF + Autogenous Demineralized Dentin Matrix

EXPERIMENTAL

Application of advanced patelet rich fibrin (A-PRF) combined with autogenous demineralized dentin matrix (ADDM) into the postoperative defect following enucleation of mandibular dentigerous cyst associated with enucleation of impacted mandibular third molars.

Procedure: Impacted third molar extraction with associated dentigerous cyst enucleation- regeneration with Advanced Platelet Rich Fibrin and Autogenous Demineralized Dentin Matrix

Interventions

Surgical extraction of impacted mandibular third molars combines with enucleation of associated dentigerous cysts performed under standarized clinical conditions.

Blood Clot Healing

Application of autologous advanced platelet rich fibrin (A-PRF) into the postoperative defect following enucleation of mandibular dentigerous cyst and extraction of associated impacted third molar to support regenerative healing.

A-PRF

Application of autologous advanced platelet rich fibrin (A-PRF) and antogenous demineralized dentin matrix (ADDM) into the postoperative defect following enucleation of mandibular dentigerous cyst and extraction of associated impacted third molar to support regenerative healing.

A-PRF + Autogenous Demineralized Dentin Matrix

Eligibility Criteria

Sexall
Healthy VolunteersNo
Age GroupsChild (0-17), Adult (18-64), Older Adult (65+)

You may qualify if:

  • Patients diagnosed with dentigerous cysts associated with impacted mandibular third molars Indication for surgical enucleation and tooth extraction Ability to provide informed consent Availability for postoperative follow-up examinations and CBCT assessment

You may not qualify if:

  • Acute local infection at the surgical site Systemic diseases affecting bone healing or tissue regeneration Immunosuppressive therapy Pregnancy or breastfeeding Hematological disorders affecting platelet function, coagulation, or fibrin formation (including thrombocytopenia, coagulopathies, or anticoagulant therapy).
  • History of radiotherapy in the head and neck region Use of medications affecting bone metabolism Heavy smoking Inability to comply with follow-up visits or postoperative recommendations

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Department of Oral Surgery, Institute of Dentistry, Jagiellonian University Medical College

Krakow, Malopolska, 31-155, Poland

Location

MeSH Terms

Conditions

Dentigerous Cyst

Condition Hierarchy (Ancestors)

Odontogenic CystsJaw CystsBone CystsCystsNeoplasmsJaw DiseasesMusculoskeletal DiseasesStomatognathic Diseases

Study Officials

  • Mariusz Szuta, MD, PhD

    Department of Oral Surgery, Institute of Dentistry, Jagiellonian University Medical College

    PRINCIPAL INVESTIGATOR
  • Tomasz Kaczmarzyk

    Department of Oral Surgery, Institute of Dentistry, Jagiellonian University Medical College

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
NONE
Purpose
TREATMENT
Intervention Model
PARALLEL
Model Details: Participants were randomly assigned in parallel to one of three study groups following enucleation of mandibular dentigerous cysts associated with impacted mandibular third molars. Group A underwent healing on a natural blood clot, Group B received advanced platelet-rich fibrin (A-PRF), and Group C received A-PRF combined with autogenous demineralized dentin matrix. Postoperative pain intensity was assessed at predefined postoperative time points using a Numerical Rating Scale (NRS) with graphical visualisation.
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
MD, PhD

Study Record Dates

First Submitted

June 11, 2026

First Posted

June 17, 2026

Study Start

April 4, 2024

Primary Completion (Estimated)

December 1, 2026

Study Completion (Estimated)

April 1, 2027

Last Updated

June 17, 2026

Record last verified: 2026-06

Locations