NCT07648381

Brief Summary

In infertility treatment, embryos are created in vitro after eggs have been fertilised by sperm using in vitro fertilisation or intracytoplasmic sperm injection techniques. These embryos, resulting from hormonal ovarian stimulation, can be transferred shortly after egg retrieval (fresh transfer) or frozen for later use. Both natural menstrual cycles and artificial cycles (using hormones to mimic a normal cycle) can prepare the uterus for embryo transfer. Patients with regular menstrual cycles often prefer the non-medicated approach. However, these cycles offer less flexibility in timing because they rely on ovulation. As the number of FET cycles increases worldwide, scientists are exploring the possibility of inducing ovulation in smaller follicles to increase the flexibility of natural cycles. However, the safety for future pregnancies is unknown. This study aims to better understand the function of the corpus luteum, the follicle remnant in the ovary after ovulation, by measuring several factors that change throughout the cycle due to substances produced by the corpus luteum. The results of the study will have a direct impact on clinical practice by increasing the flexibility of frozen transfer cycles.

Trial Health

63
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Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
118

participants targeted

Target at P50-P75 for all trials

Timeline
26mo left

Started Jul 2026

Typical duration for all trials

Geographic Reach
1 country

1 active site

Status
not yet recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

June 9, 2026

Completed
6 days until next milestone

First Posted

Study publicly available on registry

June 15, 2026

Completed
16 days until next milestone

Study Start

First participant enrolled

July 1, 2026

Expected
1.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

February 1, 2028

7 months until next milestone

Study Completion

Last participant's last visit for all outcomes

September 1, 2028

Last Updated

June 15, 2026

Status Verified

June 1, 2026

Enrollment Period

1.6 years

First QC Date

June 9, 2026

Last Update Submit

June 9, 2026

Conditions

Outcome Measures

Primary Outcomes (1)

  • The relationship between progesterone levels and different follicular sizes on day 7 post ovulation induction.

    Progesterone levels will be measured 7 days after ovulation induction to relate them to different follicular sizes.

    From the start of the menstrual cycle until 7 days after ovulation induction.

Secondary Outcomes (2)

  • Estradiol evolution throughout the cycle and its relation to luteal progesterone levels.

    From the start of a menstrual cycle until 7 days post-ovulation induction.

  • Indirect factors reflecting cardiovascular and renal adaptation in the luteal phase

    From the start of the menstrual cycle until 7 days after ovulation induction.

Study Arms (8)

Follicle size 11mm

Patients with a follicle of 11mm when measured through transvaginal ultrasound in 2D, at time of ovulation induction.

Diagnostic Test: Blood samples for the analysis of biomarkers.Diagnostic Test: Transvaginal ultrasound for follicle and endometrium measurements.Diagnostic Test: Mean Arterial Pressure measurement.Drug: Ovitrelle injection for ovulation induction

Follicle size 12 mm

Patients with a follicle of 12mm when measured through transvaginal ultrasound in 2D, at time of ovulation induction.

Diagnostic Test: Blood samples for the analysis of biomarkers.Diagnostic Test: Transvaginal ultrasound for follicle and endometrium measurements.Diagnostic Test: Mean Arterial Pressure measurement.Drug: Ovitrelle injection for ovulation induction

Follicle size 13 mm

Patients with a follicle of 13mm when measured through transvaginal ultrasound in 2D, at time of ovulation induction.

Diagnostic Test: Blood samples for the analysis of biomarkers.Diagnostic Test: Transvaginal ultrasound for follicle and endometrium measurements.Diagnostic Test: Mean Arterial Pressure measurement.Drug: Ovitrelle injection for ovulation induction

Follicle size 14 mm

Patients with a follicle of 14mm when measured through transvaginal ultrasound in 2D, at time of ovulation induction.

Diagnostic Test: Blood samples for the analysis of biomarkers.Diagnostic Test: Transvaginal ultrasound for follicle and endometrium measurements.Diagnostic Test: Mean Arterial Pressure measurement.Drug: Ovitrelle injection for ovulation induction

Follicle size 15 mm

Patients with a follicle of 15mm when measured through transvaginal ultrasound in 2D, at time of ovulation induction.

Diagnostic Test: Blood samples for the analysis of biomarkers.Diagnostic Test: Transvaginal ultrasound for follicle and endometrium measurements.Diagnostic Test: Mean Arterial Pressure measurement.Drug: Ovitrelle injection for ovulation induction

Follicle size 16 mm

Patients with a follicle of 16mm when measured through transvaginal ultrasound in 2D, at time of ovulation induction.

Diagnostic Test: Blood samples for the analysis of biomarkers.Diagnostic Test: Transvaginal ultrasound for follicle and endometrium measurements.Diagnostic Test: Mean Arterial Pressure measurement.Drug: Ovitrelle injection for ovulation induction

Follicle size 17 mm

Patients with a follicle of 17mm when measured through transvaginal ultrasound in 2D, at time of ovulation induction.

Diagnostic Test: Blood samples for the analysis of biomarkers.Diagnostic Test: Transvaginal ultrasound for follicle and endometrium measurements.Diagnostic Test: Mean Arterial Pressure measurement.Drug: Ovitrelle injection for ovulation induction

Follicle size 18 mm

Patients with a follicle of 18mm when measured through transvaginal ultrasound in 2D, at time of ovulation induction.

Diagnostic Test: Blood samples for the analysis of biomarkers.Diagnostic Test: Transvaginal ultrasound for follicle and endometrium measurements.Diagnostic Test: Mean Arterial Pressure measurement.Drug: Ovitrelle injection for ovulation induction

Interventions

We will collect several blood samples: * One at day 8 of the cycle: measurements of P, E2, Na, K, CL, CO2, Osmolality, PRL, direct renin, PAC, Nt-proBNP, Relaxin-2 and VEGF. * One for cycle follow up between day 8 and ovulation: measurement of P, E2, LH * One at day 7 after ovulation induction: measurements of P, E2, Na, K, CL, CO2, Osmolality, PRL, direct renin, PAC, Nt-proBNP, Relaxin-2 and VEGF.

Follicle size 11mmFollicle size 12 mmFollicle size 13 mmFollicle size 14 mmFollicle size 15 mmFollicle size 16 mmFollicle size 17 mmFollicle size 18 mm

A transvaginal ultrasound will be performed after day 8 of the cycle to evaluate a 2D measurement of the growing follicle, as well as a measurement of the endometrial thickness to evaluate readiness for ovulation induction.

Follicle size 11mmFollicle size 12 mmFollicle size 13 mmFollicle size 14 mmFollicle size 15 mmFollicle size 16 mmFollicle size 17 mmFollicle size 18 mm

The MAP will be measured at cycle day 7 and 7 days post-ovulation induction to follow cardiovascular adaptations.

Follicle size 11mmFollicle size 12 mmFollicle size 13 mmFollicle size 14 mmFollicle size 15 mmFollicle size 16 mmFollicle size 17 mmFollicle size 18 mm

When patient is deemed ready for ovulation based on follicle size, blood results and endometrium, she will be asked to inject Ovitrelle 250 micrograms to induce ovulation in a controlled and timed manner.

Follicle size 11mmFollicle size 12 mmFollicle size 13 mmFollicle size 14 mmFollicle size 15 mmFollicle size 16 mmFollicle size 17 mmFollicle size 18 mm

Eligibility Criteria

Age18 Years - 40 Years
Sexfemale(Gender-based eligibility)
Gender Eligibility DetailsOur study can only include people who have a menstrual cycle, as this is needed for our measurements and is our specific study population.
Healthy VolunteersYes
Age GroupsAdult (18-64)
Sampling MethodProbability Sample
Study Population

Our study population pertains to female patients and volunteers, of the age between 18 years old and 40 years old, with a regular menstrual cycle (25-35 days) and a normal BMI (18.5-35). You are eligible if you have not used any form of hormonal contraception or copper IUD throughout the last 3 months, have not had an ovarian stimulation during this time period, and are not known with renal or cardiac disease, diabetes or hypertension.

You may qualify if:

  • Age below 40 years
  • Regular menstrual cycles (between 25-35 days)
  • Normal BMI between 18.5 and 35

You may not qualify if:

  • Underlying renal or cardiac disease
  • Hypertension
  • Diabetes mellitus
  • Contraceptive use less than 3 months before MOCK cycle, including: IUD (hormonal and copper), hormonal patch, contraceptive pill (combination and mini), hormonal intravaginal ring, contraceptive implant, contraceptive injection.
  • Ovarian stimulation during the previous three months
  • History of recurrent pregnancy loss (defined as 2 or more previous pregnancy losses before 24 weeks gestation).

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

UZ Brussel

Jette, 1090, Belgium

Location

Biospecimen

Retention: SAMPLES WITHOUT DNA

Blood samples.

MeSH Terms

Interventions

Blood Specimen CollectionChorionic GonadotropinOvulation Induction

Intervention Hierarchy (Ancestors)

Specimen HandlingClinical Laboratory TechniquesDiagnostic Techniques and ProceduresDiagnosisPuncturesSurgical Procedures, OperativeInvestigative TechniquesGonadotropinsPeptide HormonesHormonesHormones, Hormone Substitutes, and Hormone AntagonistsPlacental HormonesPeptidesAmino Acids, Peptides, and ProteinsPregnancy ProteinsProteinsReproductive Techniques, AssistedReproductive TechniquesTherapeutics

Central Study Contacts

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Doctor

Study Record Dates

First Submitted

June 9, 2026

First Posted

June 15, 2026

Study Start (Estimated)

July 1, 2026

Primary Completion (Estimated)

February 1, 2028

Study Completion (Estimated)

September 1, 2028

Last Updated

June 15, 2026

Record last verified: 2026-06

Locations