NCT07597213

Brief Summary

The goal of this clinical trial is to evaluate whether hyaluronic acid (HA) improves periodontal healing in people with periodontal disease who smoke. Moreover, the effects of HA on biomolecules and bacteria levels will be assesed during the follow up period. The main questions it will answer are: Will periodontal sites treated with HA gel after non-surgical periodontal treatment (NSPT) lead to better outcomes in clinical parameters compared to the sites treated with NSPT only in smokers? Will adjunctive use of HA gel reduce oxidative stress markers and bacteria levels during follow-up? Researchers will compare periodontal sites receiving NSPT with adjunctive HA gel application to sites receiving NSPT alone to determine whether HA provides additional clinical, biochemical and microbiological benefits. Participants will:

  • first receive full-mouth NSPT then, randomization will be performed in selected jaw to determine the test sites. These two interproximal test sites will additionally receive HA gel application.
  • attend follow-up visits at 1, 3 and 6 months for clinical periodontal measurements and to provide gingival crevicular fluid samples (GCF) and subgingival samples. The GCF samples will be evaluted for Metallothionein (MT) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels and subgingival samples for periodontal pathogens.

Trial Health

63
Monitor

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
23

participants targeted

Target at below P25 for not_applicable

Timeline
25mo left

Started Jun 2026

Typical duration for not_applicable

Geographic Reach
1 country

1 active site

Status
not yet recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

May 13, 2026

Completed
6 days until next milestone

First Posted

Study publicly available on registry

May 19, 2026

Completed
13 days until next milestone

Study Start

First participant enrolled

June 1, 2026

Expected
2.1 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

July 1, 2028

Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

July 1, 2028

Last Updated

May 19, 2026

Status Verified

May 1, 2026

Enrollment Period

2.1 years

First QC Date

May 13, 2026

Last Update Submit

May 13, 2026

Conditions

Keywords

hyaluronic acidperiodontitisperiodontal pathogensmetallothionein8-OHdGNon-surgical periodontal treatmentsplit-mouth study

Outcome Measures

Primary Outcomes (2)

  • Change in Probing Depth (PD)

    PD will be measured around each tooth by recording the distance in milimeters from the gingival margin to the bottom of the pocket at 6 locations (mesiobuccal, mid-buccal, distobuccal, mesiopalatal, mid-palatal, distopalatal)

    Baseline to 1, 3 and 6 months after NSPT.

  • Change in Clinical Attachment Level

    Clinical attachment level will be measured in millimeters using a periodontal probe. The change in CAL will be calculated as the difference between baseline and follow-up measurements. A decrease in CAL indicates an improvement in periodontal attachment.

    Baseline to 1, 3 and 6 months after NSPT

Secondary Outcomes (10)

  • Change in Bleeding on Probing (%)

    Baseline to 1, 3 and 6 months after NSPT.

  • Change in Plaque Index

    Baseline to 1, 3 and 6 months after NSPT.

  • Change in Gingival Index

    Baseline to 1, 3 and 6 months after NSPT.

  • Change in GCF Metallothionein Levels

    Baseline to 1, 3 and 6 months after NSPT

  • Change in GCF 8-OHdG levels

    Baseline to 1, 3 and 6 months after NSPT

  • +5 more secondary outcomes

Study Arms (2)

Test

ACTIVE COMPARATOR

Non-surgical periodontal treatment and Hyaluronic Acid gel

Procedure: Scaling and Root PlaningDevice: Hyaluronic Acid (HYADENT BG)

Control

OTHER

Non-surgical periodontal treatment alone

Procedure: Scaling and Root Planing

Interventions

Scaling and root planing is a conventional gold-standard treatment for periodontitis. As part of this procedure, subgingival plaque and tartar are removed and root surfaces are planed.

ControlTest

Hyaluronic acid will be locally applied to periodontal pockets as an adjunct to non-surgical periodontal treatment, aiming to support periodontal healing. After completion of scaling and root planing, the material will be delivered subgingivally to designated test sites.

Test

Eligibility Criteria

Age18 Years - 65 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Individuals who sign the informed consent form
  • Participants who are systemically healthy
  • Participants who smoke (for \>5 years and ≥10 cigarettes per day)
  • Participants diagnosed with Stage III or Stage IV periodontitis
  • Participants with at least 20 natural teeth present in the mouth (excluding third molars)
  • Participants in either of the jaws, in both quadrants, having two non-adjacent interproximal sites with probing depth (PD) and clinical attachment level (CAL) ≥6 mm with no caries, restorations or furcation involvement

You may not qualify if:

  • Individuals who do not sign the informed consent form
  • Individuals having any systemic disease
  • Participants who have used antibiotics within the last 3 months
  • Participants have received any periodontal treatment within 6 months prior to the study
  • Participants who are pregnant or lactating

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Department of Periodontology, Faculty of Dentistry, Marmara University

Istanbul, Turkey (Türkiye)

Location

MeSH Terms

Conditions

Periodontitis

Interventions

Tooth ExfoliationRoot Planing

Condition Hierarchy (Ancestors)

Periodontal DiseasesMouth DiseasesStomatognathic Diseases

Intervention Hierarchy (Ancestors)

Dental Physiological PhenomenaDigestive System and Oral Physiological PhenomenaDental ScalingDental ProphylaxisPeriodonticsDentistrySubgingival CurettagePreventive Dentistry

Central Study Contacts

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, OUTCOMES ASSESSOR
Purpose
TREATMENT
Intervention Model
PARALLEL
Model Details: Split-mouth design
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

May 13, 2026

First Posted

May 19, 2026

Study Start (Estimated)

June 1, 2026

Primary Completion (Estimated)

July 1, 2028

Study Completion (Estimated)

July 1, 2028

Last Updated

May 19, 2026

Record last verified: 2026-05

Locations