NCT07579507

Brief Summary

The goal of this project is to characterise the heterogeneity of all cell populations (tumour cells, stromal and immune microenvironment) present in the tumor and their normal (and OPMD) counterparts by scRNAseq in OSCC patients. Additionally, the study will evaluate the effectiveness of non-invasive cytobrushes as a diagnostic tool compared to traditional biopsies.

Trial Health

63
Monitor

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
150

participants targeted

Target at P75+ for not_applicable

Timeline
107mo left

Started Jun 2026

Longer than P75 for not_applicable

Geographic Reach
1 country

1 active site

Status
not yet recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

May 5, 2026

Completed
7 days until next milestone

First Posted

Study publicly available on registry

May 12, 2026

Completed
20 days until next milestone

Study Start

First participant enrolled

June 1, 2026

Completed
8.8 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

March 1, 2035

Expected
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

March 1, 2035

Last Updated

May 12, 2026

Status Verified

May 1, 2026

Enrollment Period

8.8 years

First QC Date

May 5, 2026

Last Update Submit

May 5, 2026

Conditions

Keywords

scRNAseqHeterogeneity of all cell populationOPMDOSCCcytobrushRNAseq bulk

Outcome Measures

Primary Outcomes (1)

  • Characterization of the heterogeneity of all cell populations (tumor cells, stromal and immune microenvironment) in OSCC and OPMD using scRNA-seq.

    Evaluation of transcriptomic data from all cell populations to define gene expression profiles and specific signatures.

    4 years

Secondary Outcomes (4)

  • Description of the functional interactions among tumor, stromal, and immune subpopulations.

    4 years

  • Correlation between refined patient stratification (based on tumor, stromal and immune sub-population) and the impact on the response to ex-vivo treatments.

    4 years

  • Identification of prognostic and predictive biomarkers for oral squamous cell carcinoma evolution.

    4 years

  • Evaluation of cytobrushing as a non-invasive sampling method for diagnostic yield equivalence to tissue biopsy in OPMD patients.

    4 years

Study Arms (3)

OSCC clinical-biological cohort

OTHER

A clinical-biological cohort of 50 patients with OSCC. Blood sample and biospecimen at the time of a standard surgery.

Procedure: BiospecimenProcedure: Blood sampling

OPMD clinical-biological cohort

OTHER

A clinical-biological cohort of 50 patients with OPMD. Biospecimen at the time of a standard care.

Procedure: Biospecimen

Cyto-OPMD clinical-biological cohort

OTHER

A clinical-biological cohort of 50 patients with OPMD (Cyto-OPMD). Biospecimen via cytobrush and biopsy at the time of a standard care.

Procedure: Biospecimen

Interventions

Blood sampling (6 mL), taken from a routine biological exam.

OSCC clinical-biological cohort
BiospecimenPROCEDURE

* 1 or 2 tumoral specimen (depending on the size of the tumor). * 1 specimen of the healthy oral mucosa. * 1 OPMD specimen if applicable. The biospecimens will be collected at the time of the surgery organised for the standard routine medical care.

OSCC clinical-biological cohort

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • I1: Male or female at least 18 years old.
  • I2: For Cohort A: patients with OSCC who undergo surgery. For cohorts B and C: patients with OPMD.
  • I3: Patient who has agreed to participate in this research and sign consent.
  • I4: Patient affiliated to a medical insurance.
  • I5: Patient who have not previously received any anticancer treatment (radiotherapy, chemotherapy, or immunotherapy)

You may not qualify if:

  • NI1: For cohorts B and C: Patient at high risk of bleeding, such as those receiving anticoagulant or antiplatelet therapy, those with coagulation disorders, or those with a history of severe bleeding within the two weeks prior to enrollment.
  • NI2: Pregnant or nursing woman.
  • NI3: Contraindication to general anesthesia.
  • NI4: Suspicion of rare tumor of particular histology other than squamous cell carcinoma (Sarcoma...).
  • NI5: Patient under curatorial or guardianship or placed under the protection of justice.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Centre Léon Bérard

Lyon, 69008, France

Location

MeSH Terms

Conditions

Squamous Cell Carcinoma of Head and Neck

Interventions

Blood Specimen Collection

Condition Hierarchy (Ancestors)

Carcinoma, Squamous CellCarcinomaNeoplasms, Glandular and EpithelialNeoplasms by Histologic TypeNeoplasmsHead and Neck NeoplasmsNeoplasms by Site

Intervention Hierarchy (Ancestors)

Specimen HandlingClinical Laboratory TechniquesDiagnostic Techniques and ProceduresDiagnosisPuncturesSurgical Procedures, OperativeInvestigative Techniques

Study Officials

  • Philippe Zrounba, M.D.

    philippe.zrounba@lyon.unicancer.fr

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
BASIC SCIENCE
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

May 5, 2026

First Posted

May 12, 2026

Study Start

June 1, 2026

Primary Completion (Estimated)

March 1, 2035

Study Completion (Estimated)

March 1, 2035

Last Updated

May 12, 2026

Record last verified: 2026-05

Locations