Antibacterial Effect of Moringa Mouthwash in Children With Mixed Dentition Wearing Orthodontic Appliances

NCT07536438 | Completed

• 1 other identifier: ID P-PE-25-04
• Study Type: interventional
• Target: 60
• Locations: 1 country
• Sites: 1

Brief Summary

This randomized controlled study aims to evaluate the microbiological effectiveness of Moringa mouthwash in children with mixed dentition wearing removable orthodontic appliances. The study compares Moringa mouthwash with a control mouthwash in reducing oral microbial load and improving oral hygiene. Children will be followed for a specified period, and microbiological samples will be collected and analyzed to assess changes in bacterial levels

Conditions

Oral Microbial Load

Keywords

salivary microbiology

Outcome Measures

Primary Outcomes (2)

• Salivary Streptococcus mutans and Lactobacillus counts

  Salivary samples will be collected from children wearing removable orthodontic appliances at baseline and after the intervention period. The samples will be microbiologically analyzed to determine the colony-forming units (CFU/mL) of Streptococcus mutans and Lactobacillus spp. using standard culture methods. Changes in bacterial counts before and after using moringa mouthwash will be used to evaluate its antimicrobial effect.

  Baseline, 1 week, 1 month after intervention

• Change in Oral Microbial Count

  Reduction in the bacterial load of Streptococcus and Lactobacillus in the oral cavity.

  4 weeks

Study Arms (2)

Moringa Mouthwash Group

EXPERIMENTAL

Participants in this group used Moringa mouthwash daily in addition to standard oral hygiene practices to evaluate its effect on oral microflora.

Other: Moringa mouthwash

Control Group

ACTIVE COMPARATOR

Participants followed standard oral hygiene routine without the use of Moringa mouthwash

Behavioral: Oral Hygiene Routine

Interventions

Moringa mouthwash OTHER

Herbal mouthwash prepared from Moringa extract used daily to evaluate its effect on oral microflora in children.

Moringa Mouthwash Group

Oral Hygiene Routine BEHAVIORAL

Standard oral hygiene practices including tooth brushing instructions followed by participants.

Control Group

Eligibility Criteria

• Age: 8 Years - 12 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17)

You may qualify if:

• Age from 8 to 12 years old.
• Both sexes.

You may not qualify if:

• Children suffering from systemic illness.
• Children who are previously using mouthwashes.
• Oral prophylaxis history within the previous six months.
• Chronic periodontitis.
• Presence of other prosthetic rehabilitations.

Sponsors & Collaborators

• Al-Azhar University: lead

Study Sites (1)

Al-Azhar University - Faculty of Dentistry for Girls

Cairo, Cairo Governorate, 11528, Egypt

Location

Study Officials

• Mohamed Hassan Mostafa, Associate Professor

  Al-Azhar University

  STUDY DIRECTOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Masking Details: This is an open-label study. Participants and investigators are aware of the assigned interventions due to the nature of the study (mouthwash versus standard oral hygiene routine).
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Postgraduate Student in Pediatric Dentistry

Model Details: Participants will be randomly assigned into two parallel groups. One group will use Moringa mouthwash, while the control group will follow a standard oral hygiene routine. The effect on oral microflora will be assessed over the study period.

Study Record Dates

• First Submitted: April 11, 2026
• First Posted: April 17, 2026
• Study Start: July 28, 2025
• Primary Completion: March 1, 2026
• Study Completion: March 30, 2026
• Last Updated: April 22, 2026

Record last verified: 2026-04

Locations

EG (1)