Comparison of Heart Rate Variability Analysis Durations and Their Relationship With Blood Pressure

NCT07531992 | Not Yet Recruiting

• 1 other identifier: TAVNS7
• Study Type: interventional
• Target: 40
• Locations: 1 country
• Sites: 1

Brief Summary

This observational study aims to compare heart rate variability (HRV) parameters obtained from different analysis durations in healthy adults and to examine how these parameters relate to resting blood pressure values. HRV is a noninvasive measure commonly used to assess autonomic nervous system activity. Standard short-term HRV analysis is typically based on 5-minute recordings, but shorter and longer analysis durations are also used in research and practice. This study will evaluate whether HRV values derived from 1-minute, 5-minute, 10-minute, and 20-minute analysis windows differ from one another and whether their relationships with systolic blood pressure, diastolic blood pressure, and mean arterial pressure change according to analysis duration. Participants will attend a single study visit. After a rest period, resting blood pressure and pulse will be measured, and a continuous HRV recording will be obtained using a chest strap device. The study is designed to provide methodological information on the comparability of different HRV analysis durations under the same physiological conditions.

Conditions

Heart Rate Variability

Keywords

Heart Rate Variability; HRV; Autonomic Nervous System

Outcome Measures

Primary Outcomes (5)

• RMSSD Across Different Analysis Durations

  Root mean square of successive differences (RMSSD), in milliseconds (ms), calculated from predefined 1-minute, 5-minute, 10-minute, and 20-minute analysis windows extracted from the same continuous resting RR interval recording obtained under standardized resting conditions.

  Baseline, during a single study visit

• SDNN Across Different Analysis Durations

  Standard deviation of normal-to-normal intervals (SDNN), in milliseconds (ms), calculated from predefined 1-minute, 5-minute, 10-minute, and 20-minute analysis windows extracted from the same continuous resting RR interval recording obtained under standardized resting conditions.

  Baseline, during a single study visit

• Low-Frequency Power Across Different Analysis Durations

  Low-frequency (LF) power, in ms\^2, calculated from predefined 1-minute, 5-minute, 10-minute, and 20-minute analysis windows extracted from the same continuous resting RR interval recording obtained under standardized resting conditions.

  Baseline, during a single study visit

• High-Frequency Power Across Different Analysis Durations

  High-frequency (HF) power, in ms\^2, calculated from predefined 1-minute, 5-minute, 10-minute, and 20-minute analysis windows extracted from the same continuous resting RR interval recording obtained under standardized resting conditions.

  Baseline, during a single study visit.

• LF/HF Ratio Across Different Analysis Durations

  Low-frequency/high-frequency (LF/HF) ratio calculated from predefined 1-minute, 5-minute, 10-minute, and 20-minute analysis windows extracted from the same continuous resting RR interval recording obtained under standardized resting conditions.

  Baseline, during a single study visit.

Secondary Outcomes (12)

• Agreement of RMSSD Across Different Analysis Durations

  Baseline, during a single study visit

• Agreement of SDNN Across Different Analysis Durations

  Baseline, during a single study visit

• Agreement of Low-Frequency Power Across Different Analysis Durations

  Baseline, during a single study visit

• Agreement of High-Frequency Power Across Different Analysis Durations

  Baseline, during a single study visit

• Agreement of LF/HF Ratio Across Different Analysis Durations

  Baseline, during a single study visit

Study Arms (1)

Healthy Adult Assessment Group

EXPERIMENTAL

Healthy adult participants will attend a single study visit and undergo resting hemodynamic and heart rate variability (HRV) assessment under standardized conditions. After an adaptation period, systolic blood pressure, diastolic blood pressure, and pulse will be measured using an automated oscillometric blood pressure monitor. A continuous RR interval recording will then be obtained using the Polar H10 chest strap. HRV parameters will be derived from predefined 1-minute, 5-minute, 10-minute, and 20-minute analysis windows extracted from the same recording for methodological comparison.

Other: Heart Rate Variability and Blood Pressure Assessment

Interventions

Heart Rate Variability and Blood Pressure Assessment OTHER

Participants will complete a single-visit resting physiological assessment under standardized conditions. After an adaptation period, systolic blood pressure, diastolic blood pressure, and pulse will be measured using an automated oscillometric upper-arm blood pressure monitor. Continuous RR interval data will then be recorded using the Polar H10 chest strap and analyzed with Kubios HRV software. Heart rate variability parameters will be calculated from predefined 1-minute, 5-minute, 10-minute, and 20-minute analysis windows extracted from the same continuous recording for methodological comparison.

Healthy Adult Assessment Group

Eligibility Criteria

• Age: 18 Years - 40 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy adults aged 18 to 40 years
• Willing and able to provide informed consent
• Able to comply with pre-assessment instructions, including restrictions related to caffeine intake and strenuous physical activity
• No condition preventing heart rate variability and blood pressure measurements

You may not qualify if:

• History of diagnosed cardiovascular, neurological, or autonomic nervous system disease
• Known arrhythmia or pacemaker use
• Regular use of medications that may affect heart rate variability, such as beta blockers or antiarrhythmic drugs
• Acute infection, fever, or any condition affecting general health status on the day of assessment
• Severe motion artifact during measurement or technically unusable recordings

Sponsors & Collaborators

• Sinop University: lead

Study Sites (1)

Artvin Coruh University, Artvin Vocational School, Disabled Care and Rehabilitation Laboratory, Artvin,

Artvin, Turkey (Türkiye)

Location

Related Publications (4)

• Munoz ML, van Roon A, Riese H, Thio C, Oostenbroek E, Westrik I, de Geus EJ, Gansevoort R, Lefrandt J, Nolte IM, Snieder H. Validity of (Ultra-)Short Recordings for Heart Rate Variability Measurements. PLoS One. 2015 Sep 28;10(9):e0138921. doi: 10.1371/journal.pone.0138921. eCollection 2015.

  PMID: 26414314 BACKGROUND

• Thayer JF, Yamamoto SS, Brosschot JF. The relationship of autonomic imbalance, heart rate variability and cardiovascular disease risk factors. Int J Cardiol. 2010 May 28;141(2):122-31. doi: 10.1016/j.ijcard.2009.09.543. Epub 2009 Nov 11.

  PMID: 19910061 BACKGROUND

• Heart rate variability: standards of measurement, physiological interpretation and clinical use. Task Force of the European Society of Cardiology and the North American Society of Pacing and Electrophysiology. Circulation. 1996 Mar 1;93(5):1043-65. No abstract available.

  PMID: 8598068 BACKGROUND

• Shaffer F, Ginsberg JP. An Overview of Heart Rate Variability Metrics and Norms. Front Public Health. 2017 Sep 28;5:258. doi: 10.3389/fpubh.2017.00258. eCollection 2017.

  PMID: 29034226 BACKGROUND

Central Study Contacts

Sefa Haktan Hatık, PhD

CONTACT

+90 368 271 55 28; haktanhtk@gmail.com

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: OTHER
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant professor

Study Record Dates

• First Submitted: April 6, 2026
• First Posted: April 15, 2026
• Study Start: May 1, 2026
• Primary Completion (Estimated): August 31, 2026
• Study Completion (Estimated): September 15, 2026
• Last Updated: April 15, 2026

Record last verified: 2026-04

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)