Nano Silver Effect in Rapid Wound Healing

NCT07521176 | Completed

• 1 other identifier: UDDS-2026-Perio-01
• Study Type: interventional
• Target: 12
• Locations: 1 country
• Sites: 1

Brief Summary

Silver compounds have demonstrated a positive effect in most medical fields, including periodontal therapy, particularly in their nano-formulations. These nanoparticles have exhibited a significant capacity to accelerate healing and possess antibacterial and anti-inflammatory properties, in addition to their ability to mitigate pain and postoperative complications.

Conditions

Gingival Pigmentation

Keywords

silver Crystals; Nano; Periodontal dressing; Coe-Pak dressing; Acticoat; Depigmentation; Wound Healing

Outcome Measures

Primary Outcomes (2)

• Wound Healing: Clinical Tissue Healing Index

  The Tissue Healing Index was evaluated weekly for a total of four weeks postoperatively, according to the following five grades: * Grade 1 (Very Poor): Redness (\>50%), granulation, bleeding, and pus present. * Grade 2 (Poor): Redness (\>50%), granulation, and bleeding present; no pus. * Grade 3 (Good): Redness (\<50%), no granulation, no bleeding, and no exposed connective tissue. * Grade 4 (Very Good): Redness (\<25%), no granulation, no bleeding, and no exposed connective tissue. * Grade 5 (Excellent): Healthy pink tissue, no bleeding, and complete marginal closure.

  Measured weekly, one week postoperatively until week 4 (measured 4 times).

• Epithelization: Clinical epithelialization Index

  Re-epithelialization was assessed weekly for a total of four weeks postoperatively using 1% Toluidine Blue. Deep blue staining identifies denuded connective tissue, while pale blue indicates complete healing. The clinical scale is as follows: * Grade 1: No epithelial formation. * Grade 2: Partial coverage (less than 50%). * Grade 3: Substantial coverage (more than 50%). * Grade 4: Complete re-epithelialization.

  Measured weekly, one week postoperatively until week 4 (measured 4 times).

Secondary Outcomes (2)

• Pain Assessment and Analgesic Consumption

  Measured daily from day 1 till day 7 postoperatively.

• Patient Satisfaction Index (PSI)

  recorded for one time one week after the procedure was done

Study Arms (2)

Standard Coe-PAK dressing

ACTIVE COMPARATOR

De-epithelialization of the melanin-pigmented gingiva was executed with a surgical blade, and the site was covered with the Coe-Pak dressing.

Procedure: Non eugenol zinc oxide Dressing

Nano Silver dressing

EXPERIMENTAL

De-epithelialization of the melanin-pigmented gingiva was executed with a surgical blade, and the site was covered with the Nano Silver dressing.

Procedure: Nano Silver Dressing

Interventions

Non eugenol zinc oxide Dressing PROCEDURE

A standard Non eugenol zinc oxide Dressing "Coe-PAK®" was applied after the surgical removal of gingival melanin pigmentation

Standard Coe-PAK dressing

Nano Silver Dressing PROCEDURE

A Nano Silver Dressing was Applied After the surgical removal of gingival melanin pigmentation

Nano Silver dressing

Eligibility Criteria

• Age: 20 Years - 40 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Gingival Hyperpigmentation: Presence of physiological melanin pigmentation in the maxillary anterior region, categorized as Grade 3 or 4 according to the Heden Gingival Pigmentation Index.
• Age Demographic: Patients aged 20 years or older.
• Periodontal Health: Absence of active periodontal disease (e.g., periodontitis or gingivitis).
• Gingival Biotype: Presence of a medium-to-thick gingival biotype.
• Attached Gingiva: An adequate width of attached gingiva to ensure surgical stability.
• Oral Hygiene: Demonstration of good oral hygiene with a proven ability to maintain effective plaque control throughout the study period.

You may not qualify if:

• Systemic Pigmentation: Presence of gingival discoloration secondary to systemic conditions or metabolic disorders.
• Drug-Induced Pigmentation: Hyperpigmentation resulting from the administration of specific pharmacological agents.
• Poor Oral Hygiene: Inadequate plaque control or a lack of compliance with oral self-care instructions.
• Smoking Status: Current smokers or individuals with a recent history of tobacco use, due to its confounding effect on gingival pigmentation and wound healing.
• Physiological Status: Pregnant or lactating women.
• Systemic Health \& Medication: Patients with underlying systemic diseases or those receiving medications known to influence periodontal tissue integrity or inflammatory response (e.g., immunosuppressants or calcium channel blockers).
• Periodontal Disease: Presence of active periodontitis or attachment loss.

Sponsors & Collaborators

• Damascus University: lead

Study Sites (1)

Damascus University, Faculty of Dentistry, Department of periodontology.

Damascus, Syria

Location

Related Publications (7)

• Almeida G, Marques E, De Martin AS, da Silveira Bueno CE, Nowakowski A, Cunha RS. Influence of irrigating solution on postoperative pain following single-visit endodontic treatment: randomized clinical trial. J Can Dent Assoc. 2012;78:c84.

  PMID: 22985896 BACKGROUND

• Shrivastava S, Bera T, Roy A, Singh G, Ramachandrarao P, Dash D. Retracted: Characterization of enhanced antibacterial effects of novel silver nanoparticles. Nanotechnology. 2007 May 4;18(22). doi: 10.1088/0957-4484/18/22/225103.

  PMID: 37016550 BACKGROUND

• Thirumurugan G, Veni VS, Ramachandran S, Rao JV, Dhanaraju MD. Superior wound healing effect of topically delivered silver nanoparticle formulation using eco-friendly potato plant pathogenic fungus: synthesis and characterization. J Biomed Nanotechnol. 2011 Oct;7(5):659-66. doi: 10.1166/jbn.2011.1336.

  PMID: 22195483 BACKGROUND

• Gunasekaran T, Nigusse T, Dhanaraju MD. Silver nanoparticles as real topical bullets for wound healing. J Am Coll Clin Wound Spec. 2012 Jun 4;3(4):82-96. doi: 10.1016/j.jcws.2012.05.001. eCollection 2011 Dec.

  PMID: 24527370 BACKGROUND

• Nadworny PL, Wang J, Tredget EE, Burrell RE. Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis. J Inflamm (Lond). 2010 Feb 19;7:13. doi: 10.1186/1476-9255-7-13.

  PMID: 20170497 BACKGROUND

• Hurd T, Woodmansey EJ, Watkins HMA. A retrospective review of the use of a nanocrystalline silver dressing in the management of open chronic wounds in the community. Int Wound J. 2021 Dec;18(6):753-762. doi: 10.1111/iwj.13576. Epub 2021 Mar 3.

  PMID: 33660375 BACKGROUND

• Aggarwal K, Gupta S, Sood S, Bhardwaj SB, Prashar S, Jain A. Effect of nano-crystalline silver membrane on early wound healing after periodontal surgery: A comparative randomized study. J Indian Soc Periodontol. 2021 Nov-Dec;25(6):504-509. doi: 10.4103/jisp.jisp_17_21. Epub 2021 Nov 1.

  PMID: 34898916 BACKGROUND

MeSH Terms

Conditions

Vitiligo

Condition Hierarchy (Ancestors)

Hypopigmentation; Pigmentation Disorders; Skin Diseases; Skin and Connective Tissue Diseases

Study Officials

• Tarek A Qasim, DDS MSc PhD

  Damascus University, Faculty of Dentistry, Department of Periodontology

  STUDY CHAIR

• Ara V Seferian, DDS

  Damascus University, Faculty of Dentistry, Department of Periodontology

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, CARE PROVIDER
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Model Details: A split-mouth design

Study Record Dates

• First Submitted: March 31, 2026
• First Posted: April 9, 2026
• Study Start: June 2, 2024
• Primary Completion: August 4, 2025
• Study Completion: December 22, 2025
• Last Updated: April 9, 2026

Record last verified: 2026-04

Data Sharing

• IPD Sharing: Will not share

Locations

SY (1)