Indirect Biomarkers and Exercise Induced Muscle Damage

NCT07503860 | Active Not Recruiting

• 1 other identifier: 1858/03-10-2025
• Study Type: interventional
• Target: 48
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this study is to investigate the magnitude of changes in biomarkers of exercise-induced muscle damage following an acute bout of eccentric exercise of the knee extensors performed on an isokinetic dynamometer. Moreover, these biomarkers will be assessed at different time points after the initial intervention, checking the repeated bout effect phenomenon.

Conditions

Exercise Induced Muscle Damage

Keywords

Isokinetics; eccentric exercise; muscle damage; range of motion; delayed onset muscle soreness

Outcome Measures

Primary Outcomes (6)

• Delayed onset muscle soreness

  Pain will be assessed at baseline (before the eccentric exercise bout) and again at 48 hours (2 days) after the eccentric exercise bout.

  Baseline and 48 hours after the eccentric exercise bout.

• Peak torque output

  Peak torque will be assessed at baseline (before the eccentric exercise bout) and again at 48 hours (2 days) after the eccentric exercise bout.

  Baseline and 48 hours after the eccentric exercise bout.

• Range of Motion

  The free pain range of motion will be assessed at baseline (before the eccentric exercise bout) and again at 48 hours (2 days) after the eccentric exercise bout.

  Baseline and 48 hours after the eccentric exercise bout.

• Muscle oxygenation

  Using near infrared spectroscopy, the oxygenation of the knee extensors will be assessed at baseline (before the eccentric exercise bout) and again at 48 hours (2 days) after the eccentric exercise bout.

  Baseline and 48 hours after the eccentric exercise bout.

• Creatine kinase

  Creatine kinase will be assessed at baseline (before the eccentric exercise bout) and again at 48 hours (2 days) after the eccentric exercise bout.

  Baseline and 48 hours after the eccentric exercise bout.

• Muscle thickness using ultrasound

  Participants's knee extensors muscle thickness will be assessed at baseline (before the eccentric exercise bout) and again at 48 hours (2 days) after the eccentric exercise bout.

  Baseline and 48 hours after the eccentric exercise bout.

Study Arms (2)

Initial bout of isokinetic eccentric exercise

EXPERIMENTAL

Isokinetic eccentric exercise will be performed, while before and 48h after a number of indirect biomarkers will be assessed

Other: Eccentric Exercise

Subsequent bout of isokinetic eccentric exercise

EXPERIMENTAL

Isokinetic eccentric exercise will be performed, while before and 48h after a number of indirect biomarkers will be assessed

Other: Eccentric Exercise

Interventions

Eccentric Exercise OTHER

Eccentric exercise will be performed using an isokinetic dynamometer. It will be consisted of 5 set of 15 eccentric contraction of the knee extensors at an angular velocity of 60o/s. The intensity will be maximal voluntary.

Initial bout of isokinetic eccentric exercise; Subsequent bout of isokinetic eccentric exercise

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Healthy, physically active, males

You may not qualify if:

• Musculoskeletal injuries, chronic diseases, smoking, consumption of any sports/antioxidant supplements or medication, and potential inflammatory conditions

Sponsors & Collaborators

• Vassilis Paschalis: lead

Study Sites (1)

Department of Physical Education and Sport Science, National and Kapodistrian University of Athens

Athens, Attica, 17237, Greece

Location

Related Publications (5)

• Bobotas V, Chatzinikolaou PN, Methenitis S, Doika E, Spiliopoulou P, Malliou VJ, Mpampoulis T, Terzis G, Geladas N, Paschalis V. The Effect of Exercise-Induced Muscle Damage on Muscle and Cerebral Oxygenation and Performance. Int J Sports Med. 2025 Dec;46(14):1049-1060. doi: 10.1055/a-2644-4923. Epub 2025 Jun 26.

  PMID: 40570898 BACKGROUND

• Methenitis S, Theodorou AA, Chatzinikolaou PN, Margaritelis NV, Nikolaidis MG, Paschalis V. The effects of chronic concentric and eccentric training on position sense and joint reaction angle of the knee extensors. Eur J Sport Sci. 2023 Jul;23(7):1164-1174. doi: 10.1080/17461391.2023.2184726. Epub 2023 Mar 18.

  PMID: 36815692 BACKGROUND

• Panayiotou G, Paschalis V, Nikolaidis MG, Theodorou AA, Deli CK, Fotopoulou N, Fatouros IG, Koutedakis Y, Sampanis M, Jamurtas AZ. No adverse effects of statins on muscle function and health-related parameters in the elderly: an exercise study. Scand J Med Sci Sports. 2013 Oct;23(5):556-67. doi: 10.1111/j.1600-0838.2011.01437.x. Epub 2012 Jan 31.

  PMID: 22288788 BACKGROUND

• Paschalis V, Nikolaidis MG, Giakas G, Jamurtas AZ, Owolabi EO, Koutedakis Y. Position sense and reaction angle after eccentric exercise: the repeated bout effect. Eur J Appl Physiol. 2008 May;103(1):9-18. doi: 10.1007/s00421-007-0663-9. Epub 2008 Jan 3.

  PMID: 18172668 BACKGROUND

• Margaritelis NV, Theodorou AA, Chatzinikolaou PN, Kyparos A, Nikolaidis MG, Paschalis V. Eccentric exercise per se does not affect muscle damage biomarkers: early and late phase adaptations. Eur J Appl Physiol. 2021 Feb;121(2):549-559. doi: 10.1007/s00421-020-04528-w. Epub 2020 Nov 6.

  PMID: 33156414 BACKGROUND

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Dr.

Study Record Dates

• First Submitted: February 11, 2026
• First Posted: March 31, 2026
• Study Start: August 22, 2025
• Primary Completion: November 27, 2025
• Study Completion: July 15, 2026
• Last Updated: March 31, 2026

Record last verified: 2026-02

Data Sharing

• IPD Sharing: Will not share

Locations

GR (1)