B Lymphocyte Populations in the Pulmonary Microenvironment of Patients Under Mechanical Ventilation With or Without VAP (Ventilator-Associated Pneumonia)

NCT07436780 | Not Yet Recruiting

• 1 other identifier: 87RI25_0020 (OLYMPE)
• Study Type: observational
• Target: 75
• Locations: 0 countries
• Sites: N/A

Brief Summary

Diagnosis of VAP relies on a set of non-specific clinical, biological, and imaging criteria. Understanding host-pathogen interactions and the mechanisms of deregulations leading to infection of pulmonary tissue appears essential. The aim is to qualitatively describe the B lymphocyte populations present in the pulmonary microenvironment of patients admitted to intensive care and requiring invasive mechanical ventilation

Conditions

Ventilator-associated Pneumonia

Keywords

ventilator-associated pneumonia; Intensive care medicine; host-pathogen interaction; humoral immunity

Outcome Measures

Primary Outcomes (1)

• Qualitative description of B lymphocyte populations by flow cytometry analysis with specific antibodies

  To qualitatively describe the B lymphocyte populations in the endotracheal aspirates of patients admitted to intensive care and requiring invasive mechanical ventilation. B lymphocyte subpopulations will be described using a flow cytometry method using antibodies targeting the following markers: CD93, CD62L, CD14, CXCR4, CD32, CD27, CD38, CD138, CD3, CD10, CD19, CD20, kappa light chains, lambda light chains.

  At the inclusion (T1), at diagnosis (T2 on the day of VAP diagnosis for patient who will develop it, on average 72 hours after the inclusion), at the end of the study (T3 on the day before extubation, on average 5 days after the inclusion)

Secondary Outcomes (1)

• Quantitative description of B lymphocyte populations by flow cytometry analysis with specific antibodies

  At the inclusion (T1), at diagnosis (T2 on the day of VAP diagnosis for patient who will develop it, on average 72 hours after the inclusion), at the end of the study (T3 on the day before extubation, on average 5 days after the inclusion)

Study Arms (2)

Ventilator Associated Pneumonia (VAP)

Patient who developped Ventilator Associated Pneumonia (VAP) under mechanical ventilation

Without Ventilator Associated Pneumonia

Patient who did not develop Ventilator Associated Pneumonia (VAP) under mechanical ventilation

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Patients of Limoges University Hospital admitted to emergency room

You may qualify if:

• Adult patients (≥18 years old), admitted to intensive care under mechanical ventilation for an estimated duration of at least 5 days.

You may not qualify if:

• Patients admitted for an infectious pneumonia or presenting with acute respiratory distress syndrome (ARDS). Patients in aplasia (leukocytes \< 0.5 gigal/L).

Sponsors & Collaborators

• University Hospital, Limoges: lead

Biospecimen

Retention: SAMPLES WITHOUT DNA

Respiratory samples (endotracheal aspirates)

MeSH Terms

Conditions

Pneumonia, Ventilator-Associated

Condition Hierarchy (Ancestors)

Healthcare-Associated Pneumonia; Cross Infection; Infections; Pneumonia; Respiratory Tract Infections; Lung Diseases; Respiratory Tract Diseases; Iatrogenic Disease; Disease Attributes; Pathologic Processes; Pathological Conditions, Signs and Symptoms

Study Officials

• Julien VAIDIE, Dr

  University Hospital, Limoges

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Safirah Akowanou

CONTACT

+33555058755; safirah.akowanou@chu-limoges.fr

Abdeslam BEN TALEB

CONTACT

+33555058616; abdeslam.bentaleb@chu-limoges.fr

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: December 12, 2025
• First Posted: February 27, 2026
• Study Start: January 31, 2026
• Primary Completion (Estimated): July 30, 2027
• Study Completion (Estimated): July 30, 2027
• Last Updated: February 27, 2026

Record last verified: 2026-02