A Multi-dimensional Evaluation of the Effects of Linear Accelerator X-ray Irradiation on Red Blood Cells

NCT07416045 | Recruiting

• 1 other identifier: 2024-0164
• Study Type: observational
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

This study aims to investigate the effects of linear accelerator X-ray irradiation on the structure, mechanics and functional characteristics of red blood cells recovered from patients with malignant tumors during surgery. A red blood cell damage evaluation system based on multiple-dimensional indicators is established. Through atomic force microscopy, microfluidic chips and biochemical detection methods, the changes in red blood cell membrane structure, deformability and oxygen-carrying capacity under different irradiation doses are systematically evaluated. The dose-effect relationship between irradiation dose and red blood cell damage degree is clarified, providing experimental evidence for the safe dose of irradiation processing of autologous blood during surgery.

Conditions

Erythrocyte Destruction

Keywords

Red Blood Cell; linear accelerator; Intraoperative Blood Salvage; Oncologic Surgery

Outcome Measures

Primary Outcomes (3)

• Red blood cell biochemical indicators

  The biochemical changes of the treated red blood cells were detected through methods such as blood gas analyzer and related quantitative ELISA kits.

  1day

• Mechanical characteristics of red blood cells

  The mechanical property changes of red blood cells after treatment were detected by techniques such as microtubule suction and microfluidics

  3 day

• Morphological characteristics of red blood cells

  Using scanning electron microscope (SEM) and transmission electron microscope (TEM), observe the changes in the surface structure and ultrastructure of the red blood cell membrane. Also, study the morphological alterations of red blood cells.

  1day

Secondary Outcomes (1)

• Circulating tumor cell clearance indicator

  1 day

Eligibility Criteria

• Age: 18 Years - 65 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Patients aged 18 to 65 years old who are scheduled to undergo elective surgery for malignant tumors

You may qualify if:

• Patients scheduled for elective surgery for malignant tumors
• Age: 18 - 65 years old
• It is estimated that the surgical bleeding will be greater than or equal to 500 milliliters.

You may not qualify if:

• Disorders of red blood cell production (such as aplastic anemia, megaloblastic anemia, iron deficiency anemia, leukemia, myelofibrosis, etc.)
• Diseases caused by increased destruction of red blood cells (abnormalities in red blood cell membranes such as hereditary spherocytosis and paroxysmal nocturnal hemoglobinuria; abnormalities in hemoglobin such as thalassemia; abnormalities in red blood cell-related enzymes such as glucose-6-phosphate dehydrogenase deficiency, etc.)

Sponsors & Collaborators

• Second Affiliated Hospital, School of Medicine, Zhejiang University: lead

Study Sites (1)

The Second Affiliated Hospital, School of Medicine, Zhejiang University,anesthesiology department,

Hangzhou, Zhejiang, 310009, China

RECRUITING

Central Study Contacts

Fengjiang Zhang

CONTACT

+8613858007629; zrzfj@zju.edu.cn

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: February 10, 2026
• First Posted: February 17, 2026
• Study Start: January 28, 2026
• Primary Completion (Estimated): February 26, 2027
• Study Completion (Estimated): February 26, 2027
• Last Updated: February 17, 2026

Record last verified: 2026-01

Locations

CN (1)