NCT07385508

Brief Summary

Disorders of consciousness (DOC), such as the vegetative state/unresponsive wakefulness syndrome (VS/UWS) and the minimally conscious state (MCS), represent severe clinical conditions arising from acquired brain injury (ABI), exerting a profound impact on patients, caregivers, and healthcare systems. The clinical heterogeneity, diagnostic challenges, and paucity of robust evidence on the comparative efficacy of rehabilitation interventions underscore the need for a deeper understanding of the underlying neurophysiological, cognitive, and biological mechanisms involved in consciousness recovery. This monocentric ambispective observational study aims to investigate the effects of advanced neurorehabilitation treatments-including multimodal sensory stimulation, non-invasive neuromodulation via transcranial magnetic stimulation (TMS) or transcranial direct current stimulation (tDCS), and the use of augmentative and alternative communication (AAC) devices with eye-tracking-on various facets of residual consciousness in patients diagnosed with DOC admitted to the Neurorehabilitation Unit of the IRCCS Centro Neurolesi "Bonino Pulejo" in Messina. The study design encompasses the collection of clinical and rehabilitation data from hospitalized patients undergoing treatment, both retrospectively and prospectively. Specifically, data from patients admitted and treated in the 36 months preceding study initiation, as well as those admitted and treated in the subsequent 36 months, will be included. Upon data collection, participants will be classified a posteriori as "responders" or "non-responders" based on whether they meet at least one of the following criteria: (I) an increase of ≥3 points on the Coma Recovery Scale-Revised (CRS-R) from baseline or a clinically relevant state transition (e.g., VS/UWS → MCS; MCS → emergence from MCS \[EMCS\]); (II) an increase of ≥10 points on the Functional Independence Measure (FIM) or ≥0.5 standard deviations (SD) from baseline; (III) an increase of ≥10 points on the Barthel Index or ≥0.5 SD from baseline. The primary objective is to estimate the proportion of responders and non-responders at the end of training or hospitalization. Secondary objectives include identifying variables predictive of treatment response at the end of intervention (training type/modality, aetiology, demographic factors, comorbidities), quantifying the dose-response gradient (number of sessions/session duration versus response probability), and assessing changes in neurophysiological, physiological, and biological parameters collected during hospitalization (neuroimaging examinations, neurofilament light chain \[NfL\] assays, EEG/event-related potentials \[ERP\], skin conductance response, heart rate frequency and variability, and oculomotor activity). Data will be analysed using descriptive statistics appropriate to variable distributions. The primary endpoint, defined as the proportion of responders at the end of training/hospitalization, will be evaluated using logistic regression models in an as-treated approach, reporting odds ratios and marginal estimates (risk ratios and absolute risk differences) with 95% confidence intervals (CI). Confounding will be addressed through multivariable adjustment and propensity score-based methods (inverse probability of treatment weighting \[IPTW\]/overlap weighting). Continuous secondary outcomes will be analysed with linear models or linear mixed-effects models for repeated measures. Dose-response relationships will be explored, alongside sensitivity and subgroup analyses. All analyses will be performed using R software (version 4.4.2), employing two-sided tests with a significance level of α=0.05. The study will be conducted in accordance with the Declaration of Helsinki and Good Clinical Practice (GCP) guidelines.

Trial Health

65
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Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
140

participants targeted

Target at P50-P75 for all trials

Timeline
33mo left

Started Jan 2026

Typical duration for all trials

Status
not yet recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Progress10%
Jan 2026Jan 2029

First Submitted

Initial submission to the registry

January 7, 2026

Completed
9 days until next milestone

Study Start

First participant enrolled

January 16, 2026

Completed
19 days until next milestone

First Posted

Study publicly available on registry

February 4, 2026

Completed
3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

January 16, 2029

Expected
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

January 16, 2029

Last Updated

February 4, 2026

Status Verified

December 1, 2025

Enrollment Period

3 years

First QC Date

January 7, 2026

Last Update Submit

January 29, 2026

Conditions

Keywords

Vegetative stateMinimally conscious stateNeurorehabilitationEye-trackingNeurofilamentstDCSmultimodal sensory stimulationSkin conductance response

Outcome Measures

Primary Outcomes (1)

  • Level of consciousness

    CRS-R

    From admission to 36 months or eventual discharge

Secondary Outcomes (1)

  • Neurorehabilitative training modalities

    From baseline to 36 months/discharge

Other Outcomes (1)

  • Physiological response

    From baseline to 36 months / discharge

Study Arms (1)

Responders

A patient is classified as a "responder" if they meet at least one of the following criteria relative to baseline: I. An increase of ≥3 points on the Coma Recovery Scale-Revised (CRS-R) or a clinically relevant state transition (e.g., VS/UWS → MCS; MCS → emergence from MCS \[EMCS\]). II. An increase of ≥10 points on the Functional Independence Measure (FIM) or ≥0.5 standard deviations (SD) from baseline. III. An increase of ≥10 points on the Barthel Index or ≥0.5 SD from baseline.

Device: Multidisciplinary neurorehabilitation

Interventions

Patients will undergo one or more of the specified rehabilitative treatments, depending on the medical prescriptions issued. All treatments are non-invasive in nature and are administered within the hospital facility. The methods of treatment delivery vary depending on the patient's condition.

Also known as: Transcranial direct current stimulation, Transcranial Magnetic Stimulation, Multimodal Sensory Stimulation, Augmentative and alternative communication device with Eye-Tracking
Responders

Eligibility Criteria

Age18 Years - 90 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Adult patients with a diagnosis of VS/UWS or MCS, admitted to the Neurorehabilitation/GCA Unit of the Piemonte/Casazza Hospital (IRCCS Centro Neurolesi Bonino-Pulejo) and treated according to clinical practice with one or more of the following non-invasive approaches, will be included: multimodal sensory stimulation (SS), tDCS, TMS, AAC devices with eye-tracking, standard physiotherapy/speech therapy.

You may qualify if:

  • Diagnosis of VS/MCS following ABI, according to clinical and neuroradiological findings.
  • Age between 18 and 90 years.
  • Adequate pulmonary gas exchange function (PaO₂ / FiO₂ ratio ≥ 250).
  • Stable haemodynamic profile (absence of significant arterial variations in mean arterial pressure and/or heart rate).

You may not qualify if:

  • Diagnosis of DOC due to non-structural aetiopathogenesis.
  • Having previously undergone multiple rehabilitation cycles
  • Being affected by deafness and/or blindness
  • Having unstable intracranial pressure values
  • Having cerebral perfusion values below 60 mmHg
  • Having other concomitant medical conditions that could interfere with the rehabilitation

Contact the study team to confirm eligibility.

Sponsors & Collaborators

MeSH Terms

Conditions

Consciousness DisordersPersistent Vegetative State

Interventions

Transcranial Direct Current StimulationTranscranial Magnetic StimulationEye-Tracking Technology

Condition Hierarchy (Ancestors)

Neurobehavioral ManifestationsNeurologic ManifestationsNervous System DiseasesSigns and SymptomsPathological Conditions, Signs and SymptomsNeurocognitive DisordersMental DisordersBrain Damage, ChronicBrain DiseasesCentral Nervous System DiseasesUnconsciousness

Intervention Hierarchy (Ancestors)

Electric Stimulation TherapyTherapeuticsConvulsive TherapyPsychiatric Somatic TherapiesBehavioral Disciplines and ActivitiesElectroshockPsychological TechniquesMagnetic Field TherapyEye Movement MeasurementsDiagnostic Techniques, OphthalmologicalDiagnostic Techniques and ProceduresDiagnosisElectrodiagnosis

Study Officials

  • Davide Cardile, Psychologist

    IRCCS Centro Neurolesi Bonino Pulejo

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Davide Cardile, Psychologist

CONTACT

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
OTHER
Target Duration
36 Months
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Psychologist

Study Record Dates

First Submitted

January 7, 2026

First Posted

February 4, 2026

Study Start

January 16, 2026

Primary Completion (Estimated)

January 16, 2029

Study Completion (Estimated)

January 16, 2029

Last Updated

February 4, 2026

Record last verified: 2025-12

Data Sharing

IPD Sharing
Will not share